Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04510

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04495 and BWI76_RS04500 are separated by 84 nucleotidesBWI76_RS04500 and BWI76_RS04505 overlap by 1 nucleotidesBWI76_RS04505 and BWI76_RS04510 are separated by 40 nucleotidesBWI76_RS04510 and BWI76_RS04515 are separated by 4 nucleotidesBWI76_RS04515 and BWI76_RS04520 are separated by 2 nucleotides BWI76_RS04495: BWI76_RS04495 - glutathione-regulated potassium-efflux system protein KefC, at 930,808 to 932,673 _RS04495 BWI76_RS04500: BWI76_RS04500 - dihydrofolate reductase, at 932,758 to 932,871 _RS04500 BWI76_RS04505: BWI76_RS04505 - dihydrofolate reductase, at 932,871 to 933,350 _RS04505 BWI76_RS04510: BWI76_RS04510 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 933,391 to 934,239 _RS04510 BWI76_RS04515: BWI76_RS04515 - Co2+/Mg2+ efflux protein ApaG, at 934,244 to 934,621 _RS04515 BWI76_RS04520: BWI76_RS04520 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase, at 934,624 to 935,445 _RS04520 Position (kb) 933 934 935Strain fitness (log2 ratio) -3 -2 -1 0 1at 932.486 kb on - strand, within BWI76_RS04495at 932.600 kb on + strandat 932.655 kb on + strandat 932.674 kb on + strandat 932.750 kb on + strandat 933.478 kb on + strand, within BWI76_RS04510at 933.624 kb on + strand, within BWI76_RS04510at 934.061 kb on - strand, within BWI76_RS04510at 934.344 kb on - strand, within BWI76_RS04515at 934.605 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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932,486 - BWI76_RS04495 0.90 +0.3
932,600 + -0.5
932,655 + -0.9
932,674 + +0.7
932,750 + +1.4
933,478 + BWI76_RS04510 0.10 -3.7
933,624 + BWI76_RS04510 0.27 -1.5
934,061 - BWI76_RS04510 0.79 -2.6
934,344 - BWI76_RS04515 0.26 -0.6
934,605 - -1.5

Or see this region's nucleotide sequence