Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04470

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04465 and BWI76_RS04470 are separated by 18 nucleotidesBWI76_RS04470 and BWI76_RS04475 are separated by 138 nucleotides BWI76_RS04465: BWI76_RS04465 - carbamoyl-phosphate synthase small subunit, at 922,546 to 923,694 _RS04465 BWI76_RS04470: BWI76_RS04470 - carbamoyl phosphate synthase large subunit, at 923,713 to 926,937 _RS04470 BWI76_RS04475: BWI76_RS04475 - ROK family protein, at 927,076 to 927,960 _RS04475 Position (kb) 923 924 925 926 927Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 922.828 kb on + strand, within BWI76_RS04465at 922.836 kb on + strand, within BWI76_RS04465at 922.836 kb on - strand, within BWI76_RS04465at 922.897 kb on - strand, within BWI76_RS04465at 923.052 kb on - strand, within BWI76_RS04465at 923.206 kb on + strand, within BWI76_RS04465at 923.623 kb on - strandat 923.628 kb on - strandat 923.794 kb on - strandat 923.808 kb on + strandat 923.816 kb on - strandat 923.855 kb on + strandat 923.862 kb on + strandat 923.879 kb on - strandat 923.995 kb on + strandat 923.995 kb on + strandat 924.003 kb on - strandat 924.256 kb on + strand, within BWI76_RS04470at 924.264 kb on - strandat 924.575 kb on - strand, within BWI76_RS04470at 924.717 kb on + strand, within BWI76_RS04470at 924.748 kb on - strand, within BWI76_RS04470at 924.751 kb on - strand, within BWI76_RS04470at 924.760 kb on - strand, within BWI76_RS04470at 924.914 kb on + strand, within BWI76_RS04470at 925.097 kb on + strand, within BWI76_RS04470at 925.338 kb on + strand, within BWI76_RS04470at 925.346 kb on - strand, within BWI76_RS04470at 925.547 kb on + strand, within BWI76_RS04470at 925.706 kb on + strand, within BWI76_RS04470at 925.931 kb on - strand, within BWI76_RS04470at 926.164 kb on - strand, within BWI76_RS04470at 926.332 kb on - strand, within BWI76_RS04470at 926.473 kb on + strand, within BWI76_RS04470at 926.481 kb on - strand, within BWI76_RS04470at 926.610 kb on + strand, within BWI76_RS04470at 926.817 kb on + strandat 926.817 kb on + strandat 926.903 kb on - strandat 927.074 kb on - strandat 927.433 kb on + strand, within BWI76_RS04475at 927.433 kb on + strand, within BWI76_RS04475at 927.441 kb on - strand, within BWI76_RS04475at 927.613 kb on - strand, within BWI76_RS04475at 927.747 kb on + strand, within BWI76_RS04475at 927.752 kb on + strand, within BWI76_RS04475at 927.815 kb on + strand, within BWI76_RS04475at 927.823 kb on - strand, within BWI76_RS04475at 927.907 kb on + strandat 927.910 kb on - strandat 927.915 kb on - strandat 927.922 kb on + strandat 927.930 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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922,828 + BWI76_RS04465 0.25 -4.4
922,836 + BWI76_RS04465 0.25 -4.8
922,836 - BWI76_RS04465 0.25 -3.5
922,897 - BWI76_RS04465 0.31 +0.1
923,052 - BWI76_RS04465 0.44 -3.8
923,206 + BWI76_RS04465 0.57 -1.7
923,623 - -2.3
923,628 - -1.9
923,794 - -5.3
923,808 + -3.0
923,816 - +1.1
923,855 + -2.2
923,862 + -3.3
923,879 - -1.9
923,995 + -2.1
923,995 + +1.1
924,003 - -4.1
924,256 + BWI76_RS04470 0.17 -2.7
924,264 - -1.2
924,575 - BWI76_RS04470 0.27 -2.5
924,717 + BWI76_RS04470 0.31 -1.5
924,748 - BWI76_RS04470 0.32 -3.1
924,751 - BWI76_RS04470 0.32 -3.0
924,760 - BWI76_RS04470 0.32 -4.3
924,914 + BWI76_RS04470 0.37 -4.0
925,097 + BWI76_RS04470 0.43 -4.2
925,338 + BWI76_RS04470 0.50 +0.1
925,346 - BWI76_RS04470 0.51 -4.5
925,547 + BWI76_RS04470 0.57 -3.0
925,706 + BWI76_RS04470 0.62 -4.8
925,931 - BWI76_RS04470 0.69 -4.0
926,164 - BWI76_RS04470 0.76 -4.7
926,332 - BWI76_RS04470 0.81 -5.6
926,473 + BWI76_RS04470 0.86 -0.9
926,481 - BWI76_RS04470 0.86 -3.8
926,610 + BWI76_RS04470 0.90 -4.3
926,817 + -3.4
926,817 + -3.8
926,903 - -2.0
927,074 - +0.3
927,433 + BWI76_RS04475 0.40 -0.0
927,433 + BWI76_RS04475 0.40 -1.5
927,441 - BWI76_RS04475 0.41 +0.0
927,613 - BWI76_RS04475 0.61 -2.3
927,747 + BWI76_RS04475 0.76 -0.0
927,752 + BWI76_RS04475 0.76 -0.1
927,815 + BWI76_RS04475 0.84 +0.2
927,823 - BWI76_RS04475 0.84 +0.6
927,907 + +0.4
927,910 - +1.1
927,915 - -1.8
927,922 + +0.0
927,930 - -2.2

Or see this region's nucleotide sequence