Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04255

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04245 and BWI76_RS04250 are separated by 1 nucleotidesBWI76_RS04250 and BWI76_RS04255 are separated by 3 nucleotidesBWI76_RS04255 and BWI76_RS04260 are separated by 51 nucleotidesBWI76_RS04260 and BWI76_RS04265 are separated by 77 nucleotides BWI76_RS04245: BWI76_RS04245 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 872,525 to 874,987 _RS04245 BWI76_RS04250: BWI76_RS04250 - homoserine kinase, at 874,989 to 875,918 _RS04250 BWI76_RS04255: BWI76_RS04255 - threonine synthase, at 875,922 to 877,202 _RS04255 BWI76_RS04260: BWI76_RS04260 - peroxide stress protein YaaA, at 877,254 to 878,027 _RS04260 BWI76_RS04265: BWI76_RS04265 - sodium:alanine symporter family protein, at 878,105 to 879,535 _RS04265 Position (kb) 875 876 877 878Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 874.949 kb on + strandat 875.101 kb on - strand, within BWI76_RS04250at 875.266 kb on + strand, within BWI76_RS04250at 875.274 kb on - strand, within BWI76_RS04250at 875.422 kb on - strand, within BWI76_RS04250at 875.516 kb on - strand, within BWI76_RS04250at 875.539 kb on - strandat 875.587 kb on + strand, within BWI76_RS04250at 875.595 kb on - strand, within BWI76_RS04250at 875.599 kb on + strand, within BWI76_RS04250at 875.615 kb on - strand, within BWI76_RS04250at 875.846 kb on - strandat 875.962 kb on + strandat 875.965 kb on - strandat 875.970 kb on - strandat 875.987 kb on - strandat 876.169 kb on - strand, within BWI76_RS04255at 876.213 kb on - strand, within BWI76_RS04255at 876.223 kb on + strand, within BWI76_RS04255at 876.398 kb on + strand, within BWI76_RS04255at 876.428 kb on + strand, within BWI76_RS04255at 876.620 kb on + strand, within BWI76_RS04255at 876.620 kb on + strand, within BWI76_RS04255at 876.644 kb on + strand, within BWI76_RS04255at 876.644 kb on + strand, within BWI76_RS04255at 876.812 kb on + strand, within BWI76_RS04255at 876.812 kb on + strand, within BWI76_RS04255at 876.820 kb on - strand, within BWI76_RS04255at 876.820 kb on - strand, within BWI76_RS04255at 877.744 kb on + strand, within BWI76_RS04260at 877.854 kb on - strand, within BWI76_RS04260at 877.928 kb on + strand, within BWI76_RS04260at 878.022 kb on + strandat 878.031 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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874,949 + -3.0
875,101 - BWI76_RS04250 0.12 -5.2
875,266 + BWI76_RS04250 0.30 -2.6
875,274 - BWI76_RS04250 0.31 -5.4
875,422 - BWI76_RS04250 0.47 -2.5
875,516 - BWI76_RS04250 0.57 -1.4
875,539 - -1.2
875,587 + BWI76_RS04250 0.64 -2.8
875,595 - BWI76_RS04250 0.65 -2.0
875,599 + BWI76_RS04250 0.66 -2.8
875,615 - BWI76_RS04250 0.67 -2.6
875,846 - -4.9
875,962 + -4.0
875,965 - -2.6
875,970 - -2.2
875,987 - -4.6
876,169 - BWI76_RS04255 0.19 -3.4
876,213 - BWI76_RS04255 0.23 -3.2
876,223 + BWI76_RS04255 0.23 -4.1
876,398 + BWI76_RS04255 0.37 -2.0
876,428 + BWI76_RS04255 0.40 -1.7
876,620 + BWI76_RS04255 0.54 -5.4
876,620 + BWI76_RS04255 0.54 -3.0
876,644 + BWI76_RS04255 0.56 -3.2
876,644 + BWI76_RS04255 0.56 -0.9
876,812 + BWI76_RS04255 0.69 -3.0
876,812 + BWI76_RS04255 0.69 -4.9
876,820 - BWI76_RS04255 0.70 -1.4
876,820 - BWI76_RS04255 0.70 -3.7
877,744 + BWI76_RS04260 0.63 +0.3
877,854 - BWI76_RS04260 0.78 +0.4
877,928 + BWI76_RS04260 0.87 +1.1
878,022 + -1.1
878,031 - -0.4

Or see this region's nucleotide sequence