Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04180

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04175 and BWI76_RS04180 overlap by 60 nucleotidesBWI76_RS04180 and BWI76_RS04185 are separated by 125 nucleotides BWI76_RS04175: BWI76_RS04175 - energy-dependent translational throttle protein EttA, at 859,388 to 861,055 _RS04175 BWI76_RS04180: BWI76_RS04180 - hypothetical protein, at 860,996 to 861,203 _RS04180 BWI76_RS04185: BWI76_RS04185 - murein transglycosylase, at 861,329 to 863,266 _RS04185 Position (kb) 860 861 862Strain fitness (log2 ratio) -3 -2 -1 0 1at 860.196 kb on + strand, within BWI76_RS04175at 861.021 kb on - strandat 861.062 kb on - strand, within BWI76_RS04180at 861.082 kb on + strand, within BWI76_RS04180at 861.118 kb on + strand, within BWI76_RS04180at 861.126 kb on - strand, within BWI76_RS04180at 861.131 kb on - strand, within BWI76_RS04180at 861.133 kb on + strand, within BWI76_RS04180at 861.176 kb on + strand, within BWI76_RS04180at 861.293 kb on + strandat 861.530 kb on - strand, within BWI76_RS04185at 861.687 kb on + strand, within BWI76_RS04185at 861.768 kb on - strand, within BWI76_RS04185at 861.778 kb on - strand, within BWI76_RS04185at 861.835 kb on + strand, within BWI76_RS04185at 861.897 kb on - strand, within BWI76_RS04185at 862.016 kb on + strand, within BWI76_RS04185at 862.085 kb on + strand, within BWI76_RS04185at 862.128 kb on - strand, within BWI76_RS04185at 862.161 kb on + strand, within BWI76_RS04185at 862.182 kb on - strand, within BWI76_RS04185

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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860,196 + BWI76_RS04175 0.48 +0.0
861,021 - -1.6
861,062 - BWI76_RS04180 0.32 -0.3
861,082 + BWI76_RS04180 0.41 -0.7
861,118 + BWI76_RS04180 0.59 +0.5
861,126 - BWI76_RS04180 0.62 -1.4
861,131 - BWI76_RS04180 0.65 -0.5
861,133 + BWI76_RS04180 0.66 +1.0
861,176 + BWI76_RS04180 0.87 +0.5
861,293 + +1.7
861,530 - BWI76_RS04185 0.10 -2.1
861,687 + BWI76_RS04185 0.18 -1.2
861,768 - BWI76_RS04185 0.23 +0.6
861,778 - BWI76_RS04185 0.23 +0.0
861,835 + BWI76_RS04185 0.26 +0.9
861,897 - BWI76_RS04185 0.29 -1.7
862,016 + BWI76_RS04185 0.35 -2.8
862,085 + BWI76_RS04185 0.39 -0.5
862,128 - BWI76_RS04185 0.41 +1.4
862,161 + BWI76_RS04185 0.43 -0.1
862,182 - BWI76_RS04185 0.44 -0.2

Or see this region's nucleotide sequence