Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04125

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04115 and BWI76_RS04120 overlap by 4 nucleotidesBWI76_RS04120 and BWI76_RS04125 are separated by 145 nucleotidesBWI76_RS04125 and BWI76_RS04130 are separated by 414 nucleotides BWI76_RS04115: BWI76_RS04115 - patatin family protein, at 845,881 to 846,954 _RS04115 BWI76_RS04120: BWI76_RS04120 - metal-dependent hydrolase, at 846,951 to 847,730 _RS04120 BWI76_RS04125: BWI76_RS04125 - NupC/NupG family nucleoside CNT transporter, at 847,876 to 849,153 _RS04125 BWI76_RS04130: BWI76_RS04130 - 2-deoxyribose-5-phosphate aldolase, at 849,568 to 850,347 _RS04130 Position (kb) 847 848 849 850Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 847.035 kb on + strand, within BWI76_RS04120at 847.188 kb on - strand, within BWI76_RS04120at 847.211 kb on + strand, within BWI76_RS04120at 847.559 kb on + strand, within BWI76_RS04120at 847.575 kb on - strand, within BWI76_RS04120at 847.615 kb on + strand, within BWI76_RS04120at 847.615 kb on + strandat 847.840 kb on + strandat 847.840 kb on + strandat 847.840 kb on + strandat 847.848 kb on - strandat 848.038 kb on - strand, within BWI76_RS04125at 848.161 kb on + strand, within BWI76_RS04125at 848.199 kb on - strand, within BWI76_RS04125at 848.307 kb on - strand, within BWI76_RS04125at 848.307 kb on - strand, within BWI76_RS04125at 848.379 kb on + strand, within BWI76_RS04125at 848.411 kb on - strand, within BWI76_RS04125at 848.413 kb on + strand, within BWI76_RS04125at 848.413 kb on + strand, within BWI76_RS04125at 848.442 kb on + strand, within BWI76_RS04125at 848.525 kb on - strand, within BWI76_RS04125at 848.553 kb on + strand, within BWI76_RS04125at 848.874 kb on + strand, within BWI76_RS04125at 848.956 kb on - strand, within BWI76_RS04125at 848.958 kb on + strand, within BWI76_RS04125at 848.975 kb on + strand, within BWI76_RS04125at 849.148 kb on + strandat 849.483 kb on - strandat 849.607 kb on - strandat 849.727 kb on - strand, within BWI76_RS04130at 849.761 kb on + strand, within BWI76_RS04130at 849.776 kb on + strand, within BWI76_RS04130at 849.828 kb on + strand, within BWI76_RS04130at 849.870 kb on - strand, within BWI76_RS04130at 849.928 kb on - strand, within BWI76_RS04130at 849.933 kb on - strand, within BWI76_RS04130

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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847,035 + BWI76_RS04120 0.11 +0.3
847,188 - BWI76_RS04120 0.30 +0.3
847,211 + BWI76_RS04120 0.33 +1.4
847,559 + BWI76_RS04120 0.78 -0.9
847,575 - BWI76_RS04120 0.80 +0.0
847,615 + BWI76_RS04120 0.85 -0.1
847,615 + +0.1
847,840 + -0.9
847,840 + -0.3
847,840 + +0.9
847,848 - -0.2
848,038 - BWI76_RS04125 0.13 +0.8
848,161 + BWI76_RS04125 0.22 +0.7
848,199 - BWI76_RS04125 0.25 -0.4
848,307 - BWI76_RS04125 0.34 -0.9
848,307 - BWI76_RS04125 0.34 -0.4
848,379 + BWI76_RS04125 0.39 -0.2
848,411 - BWI76_RS04125 0.42 -1.5
848,413 + BWI76_RS04125 0.42 -0.6
848,413 + BWI76_RS04125 0.42 +0.8
848,442 + BWI76_RS04125 0.44 +1.6
848,525 - BWI76_RS04125 0.51 +0.2
848,553 + BWI76_RS04125 0.53 +0.1
848,874 + BWI76_RS04125 0.78 -0.6
848,956 - BWI76_RS04125 0.85 -0.3
848,958 + BWI76_RS04125 0.85 +0.2
848,975 + BWI76_RS04125 0.86 -0.1
849,148 + +2.6
849,483 - -3.0
849,607 - -5.1
849,727 - BWI76_RS04130 0.20 -3.5
849,761 + BWI76_RS04130 0.25 -0.5
849,776 + BWI76_RS04130 0.27 -1.6
849,828 + BWI76_RS04130 0.33 -2.2
849,870 - BWI76_RS04130 0.39 -3.5
849,928 - BWI76_RS04130 0.46 -3.1
849,933 - BWI76_RS04130 0.47 -4.6

Or see this region's nucleotide sequence