Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03520 and BWI76_RS03525 are separated by 277 nucleotides BWI76_RS03525 and BWI76_RS03530 are separated by 206 nucleotides
BWI76_RS03520: BWI76_RS03520 - diguanylate cyclase, at 729,781 to 731,178
_RS03520
BWI76_RS03525: BWI76_RS03525 - tryptophanyl-tRNA synthetase, at 731,456 to 732,463
_RS03525
BWI76_RS03530: BWI76_RS03530 - tryptophan permease, at 732,670 to 733,926
_RS03530
Position (kb) 731
732
733 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 730.531 kb on + strand, within BWI76_RS03520 at 730.535 kb on - strand, within BWI76_RS03520 at 730.536 kb on + strand at 730.542 kb on - strand, within BWI76_RS03520 at 730.544 kb on - strand, within BWI76_RS03520 at 730.544 kb on - strand, within BWI76_RS03520 at 730.545 kb on - strand, within BWI76_RS03520 at 730.555 kb on - strand, within BWI76_RS03520 at 730.594 kb on + strand, within BWI76_RS03520 at 730.602 kb on - strand, within BWI76_RS03520 at 730.614 kb on - strand, within BWI76_RS03520 at 730.624 kb on - strand, within BWI76_RS03520 at 730.638 kb on + strand, within BWI76_RS03520 at 730.638 kb on + strand, within BWI76_RS03520 at 730.642 kb on + strand, within BWI76_RS03520 at 730.642 kb on + strand, within BWI76_RS03520 at 730.642 kb on + strand, within BWI76_RS03520 at 730.646 kb on - strand, within BWI76_RS03520 at 730.648 kb on + strand, within BWI76_RS03520 at 730.648 kb on + strand, within BWI76_RS03520 at 730.650 kb on + strand, within BWI76_RS03520 at 730.650 kb on - strand, within BWI76_RS03520 at 730.656 kb on - strand, within BWI76_RS03520 at 730.666 kb on - strand, within BWI76_RS03520 at 730.699 kb on + strand, within BWI76_RS03520 at 730.723 kb on - strand, within BWI76_RS03520 at 730.757 kb on + strand at 731.147 kb on + strand at 731.223 kb on - strand at 731.247 kb on - strand at 731.326 kb on - strand at 731.381 kb on - strand at 731.447 kb on - strand at 731.454 kb on + strand at 731.462 kb on - strand at 731.548 kb on - strand at 731.614 kb on + strand, within BWI76_RS03525 at 731.709 kb on + strand, within BWI76_RS03525 at 731.745 kb on + strand, within BWI76_RS03525 at 731.920 kb on - strand, within BWI76_RS03525 at 731.929 kb on + strand, within BWI76_RS03525 at 732.106 kb on - strand, within BWI76_RS03525 at 732.437 kb on + strand at 732.532 kb on + strand at 732.532 kb on + strand at 732.579 kb on + strand at 732.690 kb on + strand at 732.690 kb on + strand at 732.698 kb on - strand at 732.798 kb on - strand, within BWI76_RS03530 at 732.822 kb on - strand, within BWI76_RS03530 at 732.839 kb on - strand, within BWI76_RS03530 at 732.901 kb on - strand, within BWI76_RS03530 at 732.949 kb on - strand, within BWI76_RS03530 at 733.002 kb on + strand, within BWI76_RS03530 at 733.067 kb on - strand, within BWI76_RS03530 at 733.087 kb on - strand, within BWI76_RS03530 at 733.099 kb on + strand, within BWI76_RS03530 at 733.208 kb on + strand, within BWI76_RS03530 at 733.339 kb on + strand, within BWI76_RS03530 at 733.339 kb on + strand, within BWI76_RS03530 at 733.359 kb on + strand, within BWI76_RS03530 at 733.404 kb on - strand, within BWI76_RS03530 at 733.444 kb on + strand, within BWI76_RS03530
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 730,531 + BWI76_RS03520 0.54 -0.1 730,535 - BWI76_RS03520 0.54 -3.0 730,536 + +0.1 730,542 - BWI76_RS03520 0.54 +0.4 730,544 - BWI76_RS03520 0.55 +0.3 730,544 - BWI76_RS03520 0.55 +1.7 730,545 - BWI76_RS03520 0.55 +1.3 730,555 - BWI76_RS03520 0.55 -3.1 730,594 + BWI76_RS03520 0.58 +0.6 730,602 - BWI76_RS03520 0.59 -2.7 730,614 - BWI76_RS03520 0.60 +0.4 730,624 - BWI76_RS03520 0.60 +0.6 730,638 + BWI76_RS03520 0.61 -0.9 730,638 + BWI76_RS03520 0.61 +0.1 730,642 + BWI76_RS03520 0.62 -0.2 730,642 + BWI76_RS03520 0.62 -0.9 730,642 + BWI76_RS03520 0.62 +4.6 730,646 - BWI76_RS03520 0.62 -4.2 730,648 + BWI76_RS03520 0.62 -2.5 730,648 + BWI76_RS03520 0.62 +0.1 730,650 + BWI76_RS03520 0.62 -0.4 730,650 - BWI76_RS03520 0.62 +0.6 730,656 - BWI76_RS03520 0.63 -0.1 730,666 - BWI76_RS03520 0.63 -0.6 730,699 + BWI76_RS03520 0.66 +2.0 730,723 - BWI76_RS03520 0.67 +1.9 730,757 + -1.7 731,147 + +0.9 731,223 - +0.2 731,247 - +2.1 731,326 - +0.6 731,381 - -0.3 731,447 - +2.5 731,454 + +0.1 731,462 - +0.3 731,548 - -2.2 731,614 + BWI76_RS03525 0.16 +0.7 731,709 + BWI76_RS03525 0.25 +1.6 731,745 + BWI76_RS03525 0.29 +0.4 731,920 - BWI76_RS03525 0.46 +0.6 731,929 + BWI76_RS03525 0.47 +1.3 732,106 - BWI76_RS03525 0.64 -0.2 732,437 + -0.3 732,532 + -1.9 732,532 + -2.0 732,579 + +0.7 732,690 + -0.0 732,690 + +0.5 732,698 - -1.1 732,798 - BWI76_RS03530 0.10 -1.0 732,822 - BWI76_RS03530 0.12 -0.4 732,839 - BWI76_RS03530 0.13 -0.0 732,901 - BWI76_RS03530 0.18 +0.0 732,949 - BWI76_RS03530 0.22 -0.4 733,002 + BWI76_RS03530 0.26 +0.9 733,067 - BWI76_RS03530 0.32 +0.1 733,087 - BWI76_RS03530 0.33 -0.4 733,099 + BWI76_RS03530 0.34 +0.4 733,208 + BWI76_RS03530 0.43 -0.6 733,339 + BWI76_RS03530 0.53 -2.3 733,339 + BWI76_RS03530 0.53 +0.1 733,359 + BWI76_RS03530 0.55 +0.1 733,404 - BWI76_RS03530 0.58 +0.3 733,444 + BWI76_RS03530 0.62 +1.6
Or see this region's nucleotide sequence