Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03395 and BWI76_RS03400 are separated by 146 nucleotides BWI76_RS03400 and BWI76_RS03405 are separated by 289 nucleotides BWI76_RS03405 and BWI76_RS03410 are separated by 473 nucleotides
BWI76_RS03395: BWI76_RS03395 - glyoxalase family protein, at 699,453 to 699,887
_RS03395
BWI76_RS03400: BWI76_RS03400 - hypothetical protein, at 700,034 to 700,357
_RS03400
BWI76_RS03405: BWI76_RS03405 - DNA/RNA non-specific endonuclease, at 700,647 to 701,351
_RS03405
BWI76_RS03410: BWI76_RS03410 - hypothetical protein, at 701,825 to 702,856
_RS03410
Position (kb) 700
701
702 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 699.675 kb on + strand, within BWI76_RS03395 at 699.682 kb on + strand, within BWI76_RS03395 at 699.687 kb on + strand, within BWI76_RS03395 at 699.690 kb on - strand, within BWI76_RS03395 at 699.695 kb on + strand, within BWI76_RS03395 at 699.717 kb on + strand, within BWI76_RS03395 at 699.747 kb on - strand, within BWI76_RS03395 at 699.747 kb on - strand, within BWI76_RS03395 at 699.770 kb on - strand, within BWI76_RS03395 at 699.770 kb on - strand, within BWI76_RS03395 at 699.774 kb on + strand, within BWI76_RS03395 at 699.838 kb on + strand, within BWI76_RS03395 at 699.846 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.880 kb on + strand at 699.884 kb on + strand at 699.892 kb on - strand at 699.926 kb on - strand at 699.935 kb on + strand at 699.952 kb on - strand at 699.961 kb on + strand at 699.966 kb on + strand at 699.969 kb on - strand at 699.969 kb on - strand at 699.971 kb on + strand at 699.971 kb on + strand at 699.971 kb on + strand at 699.974 kb on - strand at 699.974 kb on - strand at 699.982 kb on + strand at 699.982 kb on + strand at 699.998 kb on - strand at 700.026 kb on - strand at 700.031 kb on - strand at 700.032 kb on + strand at 700.032 kb on + strand at 700.040 kb on - strand at 700.040 kb on - strand at 700.040 kb on - strand at 700.058 kb on + strand at 700.073 kb on - strand, within BWI76_RS03400 at 700.107 kb on - strand, within BWI76_RS03400 at 700.107 kb on - strand, within BWI76_RS03400 at 700.112 kb on - strand, within BWI76_RS03400 at 700.132 kb on + strand, within BWI76_RS03400 at 700.153 kb on + strand, within BWI76_RS03400 at 700.158 kb on + strand, within BWI76_RS03400 at 700.166 kb on - strand, within BWI76_RS03400 at 700.168 kb on + strand at 700.171 kb on - strand, within BWI76_RS03400 at 700.186 kb on + strand, within BWI76_RS03400 at 700.186 kb on + strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.205 kb on - strand, within BWI76_RS03400 at 700.213 kb on - strand, within BWI76_RS03400 at 700.227 kb on - strand, within BWI76_RS03400 at 700.227 kb on - strand, within BWI76_RS03400 at 700.235 kb on + strand, within BWI76_RS03400 at 700.259 kb on - strand, within BWI76_RS03400 at 700.263 kb on + strand, within BWI76_RS03400 at 700.269 kb on + strand, within BWI76_RS03400 at 700.277 kb on - strand, within BWI76_RS03400 at 700.283 kb on - strand, within BWI76_RS03400 at 700.283 kb on - strand, within BWI76_RS03400 at 700.292 kb on - strand, within BWI76_RS03400 at 700.315 kb on + strand, within BWI76_RS03400 at 700.323 kb on - strand, within BWI76_RS03400 at 700.323 kb on - strand, within BWI76_RS03400 at 700.333 kb on - strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.352 kb on + strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.360 kb on - strand at 700.377 kb on + strand at 700.390 kb on - strand at 700.395 kb on + strand at 700.395 kb on + strand at 700.397 kb on + strand at 700.414 kb on + strand at 700.414 kb on + strand at 700.414 kb on + strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.424 kb on - strand at 700.432 kb on - strand at 700.437 kb on + strand at 700.438 kb on + strand at 700.445 kb on - strand at 700.446 kb on - strand at 700.446 kb on - strand at 700.472 kb on + strand at 700.503 kb on - strand at 700.535 kb on + strand at 700.593 kb on - strand at 700.595 kb on + strand at 700.628 kb on + strand at 700.629 kb on + strand at 700.635 kb on + strand at 700.636 kb on + strand at 700.636 kb on - strand at 700.641 kb on + strand at 700.644 kb on - strand at 700.656 kb on + strand at 700.657 kb on + strand at 700.659 kb on - strand at 700.664 kb on - strand at 700.671 kb on - strand at 700.675 kb on + strand at 700.695 kb on - strand at 700.701 kb on + strand at 700.709 kb on - strand at 700.709 kb on - strand at 700.716 kb on - strand at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.722 kb on + strand, within BWI76_RS03405 at 700.726 kb on - strand, within BWI76_RS03405 at 700.726 kb on - strand, within BWI76_RS03405 at 700.736 kb on - strand, within BWI76_RS03405 at 700.749 kb on - strand, within BWI76_RS03405 at 700.763 kb on + strand, within BWI76_RS03405 at 700.771 kb on - strand, within BWI76_RS03405 at 700.844 kb on + strand, within BWI76_RS03405 at 700.887 kb on + strand, within BWI76_RS03405 at 700.900 kb on - strand, within BWI76_RS03405 at 700.929 kb on - strand, within BWI76_RS03405 at 701.005 kb on - strand, within BWI76_RS03405 at 701.042 kb on + strand, within BWI76_RS03405 at 701.086 kb on + strand, within BWI76_RS03405 at 701.212 kb on - strand, within BWI76_RS03405 at 701.212 kb on - strand, within BWI76_RS03405 at 701.224 kb on + strand, within BWI76_RS03405 at 701.237 kb on - strand, within BWI76_RS03405 at 701.237 kb on - strand, within BWI76_RS03405 at 701.242 kb on - strand, within BWI76_RS03405 at 701.272 kb on + strand, within BWI76_RS03405 at 701.280 kb on - strand, within BWI76_RS03405 at 701.297 kb on + strand at 701.298 kb on - strand at 701.305 kb on - strand at 701.306 kb on - strand at 701.308 kb on + strand at 701.318 kb on + strand at 701.326 kb on - strand at 701.349 kb on - strand at 701.359 kb on - strand at 701.375 kb on + strand at 701.375 kb on + strand at 701.383 kb on - strand at 701.383 kb on - strand at 701.384 kb on - strand at 701.384 kb on - strand at 701.411 kb on + strand at 701.430 kb on + strand at 701.435 kb on + strand at 701.438 kb on - strand at 701.461 kb on - strand at 701.461 kb on - strand at 701.461 kb on - strand at 701.463 kb on + strand at 701.471 kb on - strand at 701.484 kb on - strand at 701.507 kb on + strand at 701.529 kb on + strand at 701.537 kb on - strand at 701.537 kb on - strand at 701.537 kb on - strand at 701.605 kb on - strand at 701.620 kb on + strand at 701.636 kb on + strand at 701.640 kb on + strand at 701.645 kb on + strand at 701.646 kb on + strand at 701.665 kb on + strand at 701.688 kb on - strand at 701.690 kb on + strand at 701.693 kb on - strand at 701.704 kb on - strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.711 kb on + strand at 701.711 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.724 kb on - strand at 701.732 kb on - strand at 701.732 kb on - strand at 701.758 kb on + strand at 701.764 kb on - strand at 701.766 kb on - strand at 701.772 kb on - strand at 701.782 kb on + strand at 701.782 kb on + strand at 701.792 kb on + strand at 701.792 kb on + strand at 701.799 kb on - strand at 701.802 kb on - strand at 701.839 kb on + strand at 701.845 kb on - strand at 701.860 kb on + strand at 701.860 kb on + strand at 701.861 kb on + strand at 701.865 kb on + strand at 701.868 kb on - strand at 701.965 kb on - strand, within BWI76_RS03410 at 702.300 kb on + strand, within BWI76_RS03410
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 699,675 + BWI76_RS03395 0.51 +0.1 699,682 + BWI76_RS03395 0.53 -2.7 699,687 + BWI76_RS03395 0.54 -0.6 699,690 - BWI76_RS03395 0.54 -0.6 699,695 + BWI76_RS03395 0.56 -0.1 699,717 + BWI76_RS03395 0.61 -0.2 699,747 - BWI76_RS03395 0.68 +0.1 699,747 - BWI76_RS03395 0.68 -1.0 699,770 - BWI76_RS03395 0.73 -1.2 699,770 - BWI76_RS03395 0.73 -3.8 699,774 + BWI76_RS03395 0.74 +0.7 699,838 + BWI76_RS03395 0.89 +0.2 699,846 - -1.0 699,850 - -1.3 699,850 - -1.5 699,850 - -2.9 699,850 - +0.7 699,880 + +0.5 699,884 + +0.9 699,892 - +0.4 699,926 - -0.8 699,935 + +1.3 699,952 - -0.7 699,961 + +0.6 699,966 + -1.0 699,969 - +0.8 699,969 - +0.2 699,971 + +1.8 699,971 + -0.3 699,971 + -1.5 699,974 - -3.1 699,974 - -2.1 699,982 + -1.1 699,982 + -0.6 699,998 - -2.2 700,026 - -0.1 700,031 - -0.3 700,032 + +0.3 700,032 + +1.3 700,040 - +0.9 700,040 - -0.0 700,040 - +0.1 700,058 + -0.1 700,073 - BWI76_RS03400 0.12 -0.4 700,107 - BWI76_RS03400 0.23 -0.2 700,107 - BWI76_RS03400 0.23 -0.2 700,112 - BWI76_RS03400 0.24 -0.8 700,132 + BWI76_RS03400 0.30 +0.7 700,153 + BWI76_RS03400 0.37 -2.2 700,158 + BWI76_RS03400 0.38 -0.7 700,166 - BWI76_RS03400 0.41 +0.2 700,168 + -2.3 700,171 - BWI76_RS03400 0.42 +0.4 700,186 + BWI76_RS03400 0.47 +0.1 700,186 + BWI76_RS03400 0.47 -0.2 700,194 - BWI76_RS03400 0.49 -1.0 700,194 - BWI76_RS03400 0.49 +0.7 700,194 - BWI76_RS03400 0.49 +0.9 700,205 - BWI76_RS03400 0.53 +0.2 700,213 - BWI76_RS03400 0.55 -0.1 700,227 - BWI76_RS03400 0.60 +1.2 700,227 - BWI76_RS03400 0.60 -0.7 700,235 + BWI76_RS03400 0.62 +0.3 700,259 - BWI76_RS03400 0.69 +0.3 700,263 + BWI76_RS03400 0.71 -1.9 700,269 + BWI76_RS03400 0.73 -0.0 700,277 - BWI76_RS03400 0.75 -0.0 700,283 - BWI76_RS03400 0.77 -0.4 700,283 - BWI76_RS03400 0.77 -0.0 700,292 - BWI76_RS03400 0.80 -1.8 700,315 + BWI76_RS03400 0.87 -0.6 700,323 - BWI76_RS03400 0.89 +0.2 700,323 - BWI76_RS03400 0.89 +0.0 700,333 - -0.6 700,347 + +0.2 700,347 + -1.1 700,347 + +0.7 700,347 + +0.2 700,352 + -0.5 700,355 - +0.6 700,355 - -0.5 700,355 - +2.2 700,355 - +0.1 700,355 - -2.8 700,360 - -0.2 700,377 + +0.8 700,390 - -0.6 700,395 + +0.5 700,395 + -2.0 700,397 + -0.7 700,414 + -1.2 700,414 + -0.8 700,414 + -0.8 700,422 - -0.1 700,422 - +2.7 700,422 - +0.0 700,422 - +0.2 700,422 - -0.7 700,422 - +2.0 700,422 - -0.0 700,422 - +1.2 700,422 - +1.9 700,422 - +1.5 700,422 - -0.1 700,424 - -0.4 700,432 - -0.0 700,437 + -0.4 700,438 + +0.6 700,445 - +1.3 700,446 - -1.2 700,446 - +0.9 700,472 + +0.4 700,503 - +1.0 700,535 + +1.9 700,593 - -0.2 700,595 + -0.8 700,628 + +0.7 700,629 + -0.4 700,635 + +0.2 700,636 + +0.0 700,636 - -0.4 700,641 + -2.2 700,644 - -0.2 700,656 + -0.8 700,657 + -0.5 700,659 - +1.4 700,664 - -1.0 700,671 - +1.2 700,675 + +1.3 700,695 - -1.2 700,701 + +2.7 700,709 - +0.3 700,709 - +0.3 700,716 - +0.2 700,718 + BWI76_RS03405 0.10 +0.3 700,718 + BWI76_RS03405 0.10 +0.1 700,718 + BWI76_RS03405 0.10 +0.2 700,718 + BWI76_RS03405 0.10 +0.3 700,722 + BWI76_RS03405 0.11 +0.1 700,726 - BWI76_RS03405 0.11 +0.4 700,726 - BWI76_RS03405 0.11 +0.2 700,736 - BWI76_RS03405 0.13 +1.1 700,749 - BWI76_RS03405 0.14 +0.8 700,763 + BWI76_RS03405 0.16 -0.8 700,771 - BWI76_RS03405 0.18 +1.6 700,844 + BWI76_RS03405 0.28 +0.3 700,887 + BWI76_RS03405 0.34 -0.1 700,900 - BWI76_RS03405 0.36 +1.3 700,929 - BWI76_RS03405 0.40 +0.5 701,005 - BWI76_RS03405 0.51 +0.3 701,042 + BWI76_RS03405 0.56 -0.9 701,086 + BWI76_RS03405 0.62 +1.0 701,212 - BWI76_RS03405 0.80 +0.3 701,212 - BWI76_RS03405 0.80 +0.8 701,224 + BWI76_RS03405 0.82 +1.2 701,237 - BWI76_RS03405 0.84 -0.4 701,237 - BWI76_RS03405 0.84 +0.6 701,242 - BWI76_RS03405 0.84 -1.0 701,272 + BWI76_RS03405 0.89 +0.7 701,280 - BWI76_RS03405 0.90 +2.4 701,297 + +0.8 701,298 - +1.6 701,305 - +1.2 701,306 - +0.3 701,308 + -0.7 701,318 + +0.4 701,326 - +0.1 701,349 - +0.1 701,359 - +0.6 701,375 + -0.6 701,375 + -0.3 701,383 - -0.8 701,383 - +0.1 701,384 - +0.3 701,384 - +1.2 701,411 + +1.2 701,430 + +0.2 701,435 + -0.3 701,438 - -1.2 701,461 - -1.7 701,461 - -1.8 701,461 - -2.9 701,463 + -1.5 701,471 - -4.5 701,484 - -2.9 701,507 + +0.2 701,529 + -0.2 701,537 - -2.4 701,537 - -2.3 701,537 - -2.0 701,605 - -2.3 701,620 + -2.2 701,636 + +0.2 701,640 + -1.0 701,645 + +0.4 701,646 + +0.8 701,665 + +0.4 701,688 - -2.3 701,690 + +3.5 701,693 - -1.3 701,704 - -0.8 701,706 + +3.6 701,706 + +0.8 701,706 + -2.0 701,706 + -1.7 701,706 + +0.3 701,706 + +2.2 701,706 + +0.2 701,706 + +0.2 701,706 + +0.2 701,711 + -0.1 701,711 + +1.6 701,713 + -2.8 701,713 + +0.2 701,713 + +0.3 701,713 + +0.2 701,713 + +1.4 701,713 + -0.6 701,713 + -0.0 701,713 + +0.1 701,713 + +1.3 701,713 + +1.4 701,713 + +0.3 701,713 + -0.1 701,713 + -2.7 701,713 + -2.3 701,714 - -0.8 701,714 - -2.5 701,714 - -1.5 701,714 - +0.4 701,714 - -1.8 701,714 - -1.2 701,714 - -1.8 701,714 - -1.2 701,721 - +0.2 701,721 - +1.6 701,721 - -3.0 701,721 - -0.8 701,721 - -1.1 701,721 - -1.3 701,721 - -0.2 701,721 - -0.1 701,721 - -2.1 701,724 - +0.2 701,732 - -1.7 701,732 - +0.7 701,758 + -2.1 701,764 - +0.0 701,766 - -0.9 701,772 - -0.7 701,782 + -0.3 701,782 + +0.9 701,792 + +0.2 701,792 + +0.0 701,799 - -1.3 701,802 - -1.0 701,839 + -0.2 701,845 - -2.8 701,860 + -0.0 701,860 + -0.1 701,861 + -0.6 701,865 + -2.4 701,868 - -0.9 701,965 - BWI76_RS03410 0.14 -0.6 702,300 + BWI76_RS03410 0.46 -1.3
Or see this region's nucleotide sequence