Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02745

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02735 and BWI76_RS02740 overlap by 11 nucleotidesBWI76_RS02740 and BWI76_RS02745 are separated by 322 nucleotidesBWI76_RS02745 and BWI76_RS02750 are separated by 67 nucleotidesBWI76_RS02750 and BWI76_RS02755 are separated by 30 nucleotides BWI76_RS02735: BWI76_RS02735 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 554,590 to 555,333 _RS02735 BWI76_RS02740: BWI76_RS02740 - putative transcriptional regulator, at 555,323 to 555,880 _RS02740 BWI76_RS02745: BWI76_RS02745 - hypothetical protein, at 556,203 to 556,409 _RS02745 BWI76_RS02750: BWI76_RS02750 - DNA-binding transcriptional regulator AraC, at 556,477 to 557,349 _RS02750 BWI76_RS02755: BWI76_RS02755 - beta-galactosidase, at 557,380 to 559,389 _RS02755 Position (kb) 556 557Strain fitness (log2 ratio) -1 0 1at 555.207 kb on - strand, within BWI76_RS02735at 555.478 kb on - strand, within BWI76_RS02740at 555.568 kb on + strand, within BWI76_RS02740at 555.634 kb on - strand, within BWI76_RS02740at 555.683 kb on + strand, within BWI76_RS02740at 555.683 kb on + strand, within BWI76_RS02740at 555.763 kb on + strand, within BWI76_RS02740at 555.775 kb on + strand, within BWI76_RS02740at 555.798 kb on - strand, within BWI76_RS02740at 555.874 kb on + strandat 555.882 kb on - strandat 555.883 kb on - strandat 556.228 kb on - strand, within BWI76_RS02745at 556.434 kb on + strandat 556.574 kb on + strand, within BWI76_RS02750at 556.790 kb on + strand, within BWI76_RS02750at 556.844 kb on - strand, within BWI76_RS02750at 557.024 kb on - strand, within BWI76_RS02750at 557.068 kb on - strand, within BWI76_RS02750at 557.142 kb on + strand, within BWI76_RS02750at 557.145 kb on - strand, within BWI76_RS02750at 557.236 kb on + strand, within BWI76_RS02750at 557.244 kb on - strand, within BWI76_RS02750

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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555,207 - BWI76_RS02735 0.83 +0.2
555,478 - BWI76_RS02740 0.28 -0.4
555,568 + BWI76_RS02740 0.44 -0.3
555,634 - BWI76_RS02740 0.56 -0.3
555,683 + BWI76_RS02740 0.65 +0.9
555,683 + BWI76_RS02740 0.65 -0.8
555,763 + BWI76_RS02740 0.79 +1.6
555,775 + BWI76_RS02740 0.81 +0.7
555,798 - BWI76_RS02740 0.85 +0.4
555,874 + +0.2
555,882 - -0.8
555,883 - +0.3
556,228 - BWI76_RS02745 0.12 +0.7
556,434 + -0.5
556,574 + BWI76_RS02750 0.11 +0.5
556,790 + BWI76_RS02750 0.36 +0.4
556,844 - BWI76_RS02750 0.42 +0.5
557,024 - BWI76_RS02750 0.63 -1.0
557,068 - BWI76_RS02750 0.68 +1.2
557,142 + BWI76_RS02750 0.76 -0.4
557,145 - BWI76_RS02750 0.77 +0.0
557,236 + BWI76_RS02750 0.87 -0.6
557,244 - BWI76_RS02750 0.88 +0.6

Or see this region's nucleotide sequence