Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02680

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02665 and BWI76_RS02670 are separated by 4 nucleotidesBWI76_RS02670 and BWI76_RS02675 are separated by 41 nucleotidesBWI76_RS02675 and BWI76_RS02680 are separated by 146 nucleotidesBWI76_RS02680 and BWI76_RS02685 are separated by 4 nucleotidesBWI76_RS02685 and BWI76_RS02690 are separated by 139 nucleotidesBWI76_RS02690 and BWI76_RS02695 overlap by 69 nucleotides BWI76_RS02665: BWI76_RS02665 - primosomal replication protein N, at 542,874 to 543,188 _RS02665 BWI76_RS02670: BWI76_RS02670 - 30S ribosomal protein S18, at 543,193 to 543,420 _RS02670 BWI76_RS02675: BWI76_RS02675 - 50S ribosomal protein L9, at 543,462 to 543,911 _RS02675 BWI76_RS02680: BWI76_RS02680 - EamA family transporter, at 544,058 to 545,011 _RS02680 BWI76_RS02685: BWI76_RS02685 - opacity-associated protein, at 545,016 to 545,675 _RS02685 BWI76_RS02690: BWI76_RS02690 - hypothetical protein, at 545,815 to 546,012 _RS02690 BWI76_RS02695: BWI76_RS02695 - peptidyl-prolyl cis-trans isomerase, at 545,944 to 546,564 _RS02695 Position (kb) 544 545 546Strain fitness (log2 ratio) -2 -1 0 1 2at 543.723 kb on + strand, within BWI76_RS02675at 544.109 kb on + strandat 544.262 kb on + strand, within BWI76_RS02680at 544.275 kb on - strand, within BWI76_RS02680at 544.367 kb on - strand, within BWI76_RS02680at 544.880 kb on + strand, within BWI76_RS02680at 545.124 kb on + strand, within BWI76_RS02685at 545.147 kb on + strand, within BWI76_RS02685at 545.150 kb on - strand, within BWI76_RS02685at 545.207 kb on + strand, within BWI76_RS02685at 545.224 kb on + strand, within BWI76_RS02685at 545.265 kb on - strand, within BWI76_RS02685at 545.499 kb on + strand, within BWI76_RS02685at 545.507 kb on - strand, within BWI76_RS02685at 545.713 kb on + strandat 545.776 kb on - strandat 545.776 kb on - strandat 545.782 kb on + strandat 545.867 kb on + strand, within BWI76_RS02690at 545.966 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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543,723 + BWI76_RS02675 0.58 +0.6
544,109 + +0.2
544,262 + BWI76_RS02680 0.21 +2.1
544,275 - BWI76_RS02680 0.23 -0.0
544,367 - BWI76_RS02680 0.32 +1.4
544,880 + BWI76_RS02680 0.86 +0.5
545,124 + BWI76_RS02685 0.16 +0.2
545,147 + BWI76_RS02685 0.20 +0.2
545,150 - BWI76_RS02685 0.20 -1.7
545,207 + BWI76_RS02685 0.29 -0.6
545,224 + BWI76_RS02685 0.32 +0.7
545,265 - BWI76_RS02685 0.38 -0.8
545,499 + BWI76_RS02685 0.73 -0.5
545,507 - BWI76_RS02685 0.74 +0.2
545,713 + +0.9
545,776 - +0.1
545,776 - -0.4
545,782 + -0.5
545,867 + BWI76_RS02690 0.26 +0.2
545,966 + -2.0

Or see this region's nucleotide sequence