Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01085

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01080 and BWI76_RS01085 are separated by 21 nucleotidesBWI76_RS01085 and BWI76_RS01090 are separated by 56 nucleotides BWI76_RS01080: BWI76_RS01080 - undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase, at 222,214 to 223,308 _RS01080 BWI76_RS01085: BWI76_RS01085 - polysaccharide chain length modulation protein, at 223,330 to 224,376 _RS01085 BWI76_RS01090: BWI76_RS01090 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 224,433 to 225,563 _RS01090 Position (kb) 223 224 225Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 222.345 kb on - strand, within BWI76_RS01080at 222.353 kb on - strand, within BWI76_RS01080at 222.434 kb on + strand, within BWI76_RS01080at 222.435 kb on + strand, within BWI76_RS01080at 222.439 kb on + strand, within BWI76_RS01080at 222.441 kb on - strand, within BWI76_RS01080at 222.441 kb on - strand, within BWI76_RS01080at 222.447 kb on - strand, within BWI76_RS01080at 222.447 kb on - strand, within BWI76_RS01080at 222.449 kb on + strand, within BWI76_RS01080at 222.496 kb on - strand, within BWI76_RS01080at 222.612 kb on - strand, within BWI76_RS01080at 222.629 kb on + strand, within BWI76_RS01080at 222.647 kb on - strand, within BWI76_RS01080at 222.703 kb on + strand, within BWI76_RS01080at 222.762 kb on - strand, within BWI76_RS01080at 222.842 kb on + strand, within BWI76_RS01080at 222.921 kb on + strand, within BWI76_RS01080at 222.935 kb on + strand, within BWI76_RS01080at 223.009 kb on - strand, within BWI76_RS01080at 223.086 kb on + strand, within BWI76_RS01080at 223.116 kb on - strand, within BWI76_RS01080at 223.133 kb on + strand, within BWI76_RS01080at 223.238 kb on + strandat 223.261 kb on - strandat 223.301 kb on - strandat 223.325 kb on - strandat 223.326 kb on + strandat 223.334 kb on - strandat 223.419 kb on + strandat 223.438 kb on + strand, within BWI76_RS01085at 223.608 kb on + strand, within BWI76_RS01085at 223.697 kb on + strand, within BWI76_RS01085at 223.917 kb on + strand, within BWI76_RS01085at 224.006 kb on + strand, within BWI76_RS01085at 224.069 kb on + strand, within BWI76_RS01085at 224.097 kb on + strand, within BWI76_RS01085at 224.119 kb on - strand, within BWI76_RS01085at 224.184 kb on - strand, within BWI76_RS01085at 224.637 kb on - strand, within BWI76_RS01090at 224.826 kb on - strand, within BWI76_RS01090at 224.859 kb on + strand, within BWI76_RS01090at 225.000 kb on - strand, within BWI76_RS01090at 225.010 kb on + strand, within BWI76_RS01090at 225.020 kb on + strand, within BWI76_RS01090

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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222,345 - BWI76_RS01080 0.12 -0.6
222,353 - BWI76_RS01080 0.13 +0.5
222,434 + BWI76_RS01080 0.20 +0.4
222,435 + BWI76_RS01080 0.20 +2.3
222,439 + BWI76_RS01080 0.21 -1.9
222,441 - BWI76_RS01080 0.21 -0.7
222,441 - BWI76_RS01080 0.21 -2.6
222,447 - BWI76_RS01080 0.21 +0.1
222,447 - BWI76_RS01080 0.21 -1.0
222,449 + BWI76_RS01080 0.21 -2.9
222,496 - BWI76_RS01080 0.26 +0.3
222,612 - BWI76_RS01080 0.36 +0.6
222,629 + BWI76_RS01080 0.38 -2.9
222,647 - BWI76_RS01080 0.40 -0.1
222,703 + BWI76_RS01080 0.45 +1.2
222,762 - BWI76_RS01080 0.50 -0.9
222,842 + BWI76_RS01080 0.57 -0.5
222,921 + BWI76_RS01080 0.65 -1.3
222,935 + BWI76_RS01080 0.66 -0.5
223,009 - BWI76_RS01080 0.73 +0.8
223,086 + BWI76_RS01080 0.80 -2.5
223,116 - BWI76_RS01080 0.82 -3.0
223,133 + BWI76_RS01080 0.84 -0.2
223,238 + +0.2
223,261 - +0.3
223,301 - -0.9
223,325 - +0.6
223,326 + -3.5
223,334 - -1.6
223,419 + +1.3
223,438 + BWI76_RS01085 0.10 -2.7
223,608 + BWI76_RS01085 0.27 -0.3
223,697 + BWI76_RS01085 0.35 -1.3
223,917 + BWI76_RS01085 0.56 -0.4
224,006 + BWI76_RS01085 0.65 -0.6
224,069 + BWI76_RS01085 0.71 -0.5
224,097 + BWI76_RS01085 0.73 -0.3
224,119 - BWI76_RS01085 0.75 +0.1
224,184 - BWI76_RS01085 0.82 -1.2
224,637 - BWI76_RS01090 0.18 +2.9
224,826 - BWI76_RS01090 0.35 +0.4
224,859 + BWI76_RS01090 0.38 +1.0
225,000 - BWI76_RS01090 0.50 -1.3
225,010 + BWI76_RS01090 0.51 -0.7
225,020 + BWI76_RS01090 0.52 -0.4

Or see this region's nucleotide sequence