Experiment: KB with Peucenin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0081 and Psyr_0082 are separated by 2 nucleotides Psyr_0082 and Psyr_0083 are separated by 13 nucleotides Psyr_0083 and Psyr_0084 are separated by 94 nucleotides
Psyr_0081: Psyr_0081 - ABC transporter, at 87,481 to 88,461
_0081
Psyr_0082: Psyr_0082 - Binding-protein-dependent transport systems inner membrane component, at 88,464 to 89,336
_0082
Psyr_0083: Psyr_0083 - Binding-protein-dependent transport systems inner membrane component, at 89,350 to 90,171
_0083
Psyr_0084: Psyr_0084 - extracellular solute-binding protein, family 1, at 90,266 to 91,276
_0084
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 87.483 kb on - strand at 87.483 kb on - strand at 87.788 kb on + strand, within Psyr_0081 at 87.789 kb on - strand, within Psyr_0081 at 88.465 kb on + strand at 88.465 kb on + strand at 88.466 kb on - strand at 88.482 kb on - strand at 88.588 kb on + strand, within Psyr_0082 at 88.588 kb on + strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.695 kb on - strand, within Psyr_0082 at 88.712 kb on - strand, within Psyr_0082 at 88.963 kb on + strand, within Psyr_0082 at 88.963 kb on + strand, within Psyr_0082 at 88.963 kb on + strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 89.044 kb on + strand, within Psyr_0082 at 89.044 kb on + strand, within Psyr_0082 at 89.045 kb on - strand, within Psyr_0082 at 89.045 kb on - strand, within Psyr_0082 at 89.239 kb on - strand, within Psyr_0082 at 89.329 kb on + strand at 89.329 kb on + strand at 89.329 kb on + strand at 89.329 kb on + strand at 89.330 kb on - strand at 89.330 kb on - strand at 89.330 kb on - strand at 89.450 kb on + strand, within Psyr_0083 at 89.450 kb on + strand, within Psyr_0083 at 89.451 kb on - strand, within Psyr_0083 at 89.451 kb on - strand, within Psyr_0083 at 89.819 kb on + strand, within Psyr_0083 at 89.819 kb on + strand, within Psyr_0083 at 89.820 kb on - strand, within Psyr_0083 at 89.913 kb on - strand, within Psyr_0083 at 89.970 kb on - strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.085 kb on + strand, within Psyr_0083 at 90.085 kb on + strand, within Psyr_0083 at 90.088 kb on + strand, within Psyr_0083 at 90.088 kb on + strand, within Psyr_0083 at 90.089 kb on - strand at 90.107 kb on + strand at 90.107 kb on + strand at 90.107 kb on + strand at 90.108 kb on - strand at 90.108 kb on - strand at 90.108 kb on - strand at 90.108 kb on - strand at 90.119 kb on + strand at 90.119 kb on + strand at 90.159 kb on + strand at 90.160 kb on - strand at 90.171 kb on + strand at 90.171 kb on + strand at 90.172 kb on - strand at 90.172 kb on - strand at 90.172 kb on - strand at 90.172 kb on - strand at 90.267 kb on + strand at 90.279 kb on + strand at 90.279 kb on + strand at 90.279 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Peucenin 10 mM remove 87,483 - -0.4 87,483 - +0.0 87,788 + Psyr_0081 0.31 -0.5 87,789 - Psyr_0081 0.31 +0.0 88,465 + -0.1 88,465 + +1.2 88,466 - -0.4 88,482 - +1.0 88,588 + Psyr_0082 0.14 -0.2 88,588 + Psyr_0082 0.14 -0.6 88,589 - Psyr_0082 0.14 -0.8 88,589 - Psyr_0082 0.14 -0.2 88,589 - Psyr_0082 0.14 +0.3 88,589 - Psyr_0082 0.14 +0.5 88,695 - Psyr_0082 0.26 -0.7 88,712 - Psyr_0082 0.28 -1.8 88,963 + Psyr_0082 0.57 -2.9 88,963 + Psyr_0082 0.57 -2.0 88,963 + Psyr_0082 0.57 -0.4 88,964 - Psyr_0082 0.57 -0.2 88,964 - Psyr_0082 0.57 -0.0 88,964 - Psyr_0082 0.57 -0.7 88,964 - Psyr_0082 0.57 -0.4 89,044 + Psyr_0082 0.66 -0.4 89,044 + Psyr_0082 0.66 -0.3 89,045 - Psyr_0082 0.67 +0.2 89,045 - Psyr_0082 0.67 +0.4 89,239 - Psyr_0082 0.89 -1.1 89,329 + +1.0 89,329 + +0.1 89,329 + -0.3 89,329 + +0.6 89,330 - -0.2 89,330 - -0.4 89,330 - -0.1 89,450 + Psyr_0083 0.12 -0.1 89,450 + Psyr_0083 0.12 -1.3 89,451 - Psyr_0083 0.12 +1.9 89,451 - Psyr_0083 0.12 +0.7 89,819 + Psyr_0083 0.57 -0.8 89,819 + Psyr_0083 0.57 -0.2 89,820 - Psyr_0083 0.57 -0.1 89,913 - Psyr_0083 0.68 -1.3 89,970 - Psyr_0083 0.75 +0.7 90,064 + Psyr_0083 0.87 -1.2 90,064 + Psyr_0083 0.87 -0.4 90,064 + Psyr_0083 0.87 -1.5 90,064 + Psyr_0083 0.87 +0.1 90,064 + Psyr_0083 0.87 +0.2 90,064 + Psyr_0083 0.87 -0.1 90,064 + Psyr_0083 0.87 +0.7 90,064 + Psyr_0083 0.87 -0.4 90,064 + Psyr_0083 0.87 +0.1 90,064 + Psyr_0083 0.87 -0.6 90,065 - Psyr_0083 0.87 +0.6 90,065 - Psyr_0083 0.87 +1.0 90,065 - Psyr_0083 0.87 +0.0 90,065 - Psyr_0083 0.87 +0.5 90,065 - Psyr_0083 0.87 +0.5 90,065 - Psyr_0083 0.87 -1.6 90,065 - Psyr_0083 0.87 -1.3 90,085 + Psyr_0083 0.89 -0.8 90,085 + Psyr_0083 0.89 +0.3 90,088 + Psyr_0083 0.90 -0.7 90,088 + Psyr_0083 0.90 +0.6 90,089 - +0.3 90,107 + -0.5 90,107 + +0.3 90,107 + -0.2 90,108 - -0.2 90,108 - -0.7 90,108 - -0.9 90,108 - +0.5 90,119 + +0.4 90,119 + -0.5 90,159 + +0.5 90,160 - +0.5 90,171 + +0.7 90,171 + -1.5 90,172 - +0.2 90,172 - +0.3 90,172 - -0.1 90,172 - +0.0 90,267 + +0.1 90,279 + -1.1 90,279 + -0.5 90,279 + +0.0
Or see this region's nucleotide sequence