Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04130

Experiment: 2'-Deoxycytidine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04125 and BWI76_RS04130 are separated by 414 nucleotidesBWI76_RS04130 and BWI76_RS04135 are separated by 77 nucleotides BWI76_RS04125: BWI76_RS04125 - NupC/NupG family nucleoside CNT transporter, at 847,876 to 849,153 _RS04125 BWI76_RS04130: BWI76_RS04130 - 2-deoxyribose-5-phosphate aldolase, at 849,568 to 850,347 _RS04130 BWI76_RS04135: BWI76_RS04135 - thymidine phosphorylase, at 850,425 to 851,747 _RS04135 Position (kb) 849 850 851Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 848.874 kb on + strand, within BWI76_RS04125at 848.956 kb on - strand, within BWI76_RS04125at 848.958 kb on + strand, within BWI76_RS04125at 848.975 kb on + strand, within BWI76_RS04125at 849.148 kb on + strandat 849.483 kb on - strandat 849.607 kb on - strandat 849.727 kb on - strand, within BWI76_RS04130at 849.761 kb on + strand, within BWI76_RS04130at 849.776 kb on + strand, within BWI76_RS04130at 849.828 kb on + strand, within BWI76_RS04130at 849.870 kb on - strand, within BWI76_RS04130at 849.928 kb on - strand, within BWI76_RS04130at 849.933 kb on - strand, within BWI76_RS04130at 850.241 kb on - strand, within BWI76_RS04130at 850.396 kb on + strandat 850.652 kb on - strand, within BWI76_RS04135at 850.836 kb on + strand, within BWI76_RS04135at 851.062 kb on + strand, within BWI76_RS04135at 851.062 kb on + strand, within BWI76_RS04135at 851.077 kb on - strand, within BWI76_RS04135at 851.136 kb on + strandat 851.331 kb on + strand, within BWI76_RS04135at 851.339 kb on - strand, within BWI76_RS04135at 851.339 kb on - strand, within BWI76_RS04135

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 5 mM (C)
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848,874 + BWI76_RS04125 0.78 -0.4
848,956 - BWI76_RS04125 0.85 -0.0
848,958 + BWI76_RS04125 0.85 -0.2
848,975 + BWI76_RS04125 0.86 -0.8
849,148 + +1.4
849,483 - -3.2
849,607 - -5.3
849,727 - BWI76_RS04130 0.20 -3.7
849,761 + BWI76_RS04130 0.25 -0.6
849,776 + BWI76_RS04130 0.27 -4.8
849,828 + BWI76_RS04130 0.33 -3.6
849,870 - BWI76_RS04130 0.39 -3.7
849,928 - BWI76_RS04130 0.46 -0.9
849,933 - BWI76_RS04130 0.47 -4.7
850,241 - BWI76_RS04130 0.86 -2.5
850,396 + -1.6
850,652 - BWI76_RS04135 0.17 -5.8
850,836 + BWI76_RS04135 0.31 -4.0
851,062 + BWI76_RS04135 0.48 -3.5
851,062 + BWI76_RS04135 0.48 -4.1
851,077 - BWI76_RS04135 0.49 -2.9
851,136 + -0.0
851,331 + BWI76_RS04135 0.68 -4.6
851,339 - BWI76_RS04135 0.69 -5.0
851,339 - BWI76_RS04135 0.69 -1.9

Or see this region's nucleotide sequence