Experiment: 2'-Deoxycytidine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00435 and BWI76_RS00440 are separated by 205 nucleotides BWI76_RS00440 and BWI76_RS00445 overlap by 4 nucleotides BWI76_RS00445 and BWI76_RS00450 are separated by 37 nucleotides BWI76_RS00450 and BWI76_RS00455 are separated by 343 nucleotides BWI76_RS00455 and BWI76_RS00460 overlap by 4 nucleotides
BWI76_RS00435: BWI76_RS00435 - lipase, at 90,170 to 91,087
_RS00435
BWI76_RS00440: BWI76_RS00440 - CopG family transcriptional regulator, at 91,293 to 91,532
_RS00440
BWI76_RS00445: BWI76_RS00445 - PIN domain nuclease, at 91,529 to 91,903
_RS00445
BWI76_RS00450: BWI76_RS00450 - formate dehydrogenase accessory protein FdhE, at 91,941 to 92,870
_RS00450
BWI76_RS00455: BWI76_RS00455 - formate dehydrogenase cytochrome b556 subunit, at 93,214 to 93,849
_RS00455
BWI76_RS00460: BWI76_RS00460 - formate dehydrogenase subunit beta, at 93,846 to 94,748
_RS00460
Position (kb) 91
92
93 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 91.063 kb on + strand at 91.279 kb on + strand at 91.308 kb on + strand at 91.364 kb on + strand, within BWI76_RS00440 at 91.645 kb on - strand, within BWI76_RS00445 at 91.693 kb on + strand, within BWI76_RS00445 at 91.746 kb on + strand, within BWI76_RS00445 at 91.783 kb on + strand, within BWI76_RS00445 at 91.800 kb on + strand, within BWI76_RS00445 at 91.807 kb on + strand, within BWI76_RS00445 at 91.807 kb on + strand, within BWI76_RS00445 at 91.807 kb on + strand, within BWI76_RS00445 at 91.815 kb on - strand, within BWI76_RS00445 at 91.830 kb on + strand, within BWI76_RS00445 at 91.830 kb on + strand, within BWI76_RS00445 at 91.830 kb on + strand, within BWI76_RS00445 at 91.934 kb on - strand at 91.934 kb on - strand at 91.970 kb on + strand at 91.978 kb on - strand at 92.014 kb on + strand at 92.222 kb on + strand, within BWI76_RS00450 at 92.226 kb on + strand, within BWI76_RS00450 at 92.251 kb on - strand, within BWI76_RS00450 at 92.555 kb on + strand, within BWI76_RS00450 at 92.601 kb on + strand, within BWI76_RS00450 at 92.610 kb on - strand, within BWI76_RS00450 at 92.682 kb on - strand, within BWI76_RS00450 at 92.851 kb on - strand at 93.001 kb on - strand at 93.015 kb on + strand at 93.037 kb on + strand at 93.052 kb on - strand at 93.067 kb on + strand at 93.079 kb on + strand at 93.123 kb on + strand at 93.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 5 mM (C) remove 91,063 + +3.4 91,279 + +0.3 91,308 + +0.2 91,364 + BWI76_RS00440 0.30 -1.2 91,645 - BWI76_RS00445 0.31 +1.0 91,693 + BWI76_RS00445 0.44 -0.7 91,746 + BWI76_RS00445 0.58 -0.1 91,783 + BWI76_RS00445 0.68 +0.3 91,800 + BWI76_RS00445 0.72 +3.1 91,807 + BWI76_RS00445 0.74 +0.1 91,807 + BWI76_RS00445 0.74 -1.4 91,807 + BWI76_RS00445 0.74 -0.3 91,815 - BWI76_RS00445 0.76 -0.7 91,830 + BWI76_RS00445 0.80 -0.2 91,830 + BWI76_RS00445 0.80 +2.4 91,830 + BWI76_RS00445 0.80 +0.0 91,934 - -2.8 91,934 - +2.1 91,970 + +1.2 91,978 - +1.0 92,014 + -0.4 92,222 + BWI76_RS00450 0.30 -0.5 92,226 + BWI76_RS00450 0.31 +0.6 92,251 - BWI76_RS00450 0.33 -1.7 92,555 + BWI76_RS00450 0.66 +0.7 92,601 + BWI76_RS00450 0.71 -0.4 92,610 - BWI76_RS00450 0.72 +0.8 92,682 - BWI76_RS00450 0.80 -0.9 92,851 - -1.2 93,001 - -1.0 93,015 + +0.3 93,037 + -0.9 93,052 - -1.2 93,067 + -0.1 93,079 + +0.0 93,123 + -1.2 93,135 - +0.5
Or see this region's nucleotide sequence