Experiment: KB with Cantharidin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2894 and Psyr_RNA45 are separated by 91 nucleotides Psyr_RNA45 and Psyr_2895 are separated by 66 nucleotides Psyr_2895 and Psyr_2896 are separated by 32 nucleotides Psyr_2896 and Psyr_2897 are separated by 2 nucleotides Psyr_2897 and Psyr_2898 are separated by 145 nucleotides
Psyr_2894: Psyr_2894 - conserved hypothetical protein, at 3,473,200 to 3,473,658
_2894
Psyr_RNA45: Psyr_RNA45 - tRNA-Gly, at 3,473,750 to 3,473,825
_RNA45
Psyr_2895: Psyr_2895 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,473,892 to 3,474,452
_2895
Psyr_2896: Psyr_2896 - Excinuclease ABC subunit C, at 3,474,485 to 3,476,308
_2896
Psyr_2897: Psyr_2897 - regulatory protein, LuxR:Response regulator receiver, at 3,476,311 to 3,476,979
_2897
Psyr_2898: Psyr_2898 - Helix-turn-helix motif protein, at 3,477,125 to 3,477,487
_2898
Position (kb)
3474
3475
3476
3477 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3473.545 kb on + strand, within Psyr_2894 at 3473.546 kb on - strand, within Psyr_2894 at 3473.656 kb on + strand at 3473.656 kb on + strand at 3473.657 kb on - strand at 3473.657 kb on - strand at 3474.508 kb on - strand at 3474.508 kb on - strand at 3474.733 kb on + strand, within Psyr_2896 at 3474.793 kb on - strand, within Psyr_2896 at 3474.863 kb on + strand, within Psyr_2896 at 3474.964 kb on - strand, within Psyr_2896 at 3474.964 kb on - strand, within Psyr_2896 at 3474.996 kb on + strand, within Psyr_2896 at 3475.119 kb on + strand, within Psyr_2896 at 3475.405 kb on - strand, within Psyr_2896 at 3475.448 kb on + strand, within Psyr_2896 at 3475.448 kb on + strand, within Psyr_2896 at 3475.449 kb on - strand, within Psyr_2896 at 3475.449 kb on - strand, within Psyr_2896 at 3475.449 kb on - strand, within Psyr_2896 at 3475.736 kb on + strand, within Psyr_2896 at 3475.736 kb on + strand, within Psyr_2896 at 3475.736 kb on + strand, within Psyr_2896 at 3475.736 kb on + strand, within Psyr_2896 at 3475.736 kb on + strand, within Psyr_2896 at 3475.736 kb on + strand, within Psyr_2896 at 3475.736 kb on + strand, within Psyr_2896 at 3475.736 kb on + strand, within Psyr_2896 at 3475.736 kb on + strand, within Psyr_2896 at 3475.737 kb on - strand, within Psyr_2896 at 3475.737 kb on - strand, within Psyr_2896 at 3475.737 kb on - strand, within Psyr_2896 at 3475.737 kb on - strand, within Psyr_2896 at 3475.737 kb on - strand, within Psyr_2896 at 3475.737 kb on - strand, within Psyr_2896 at 3475.749 kb on + strand, within Psyr_2896 at 3475.749 kb on + strand, within Psyr_2896 at 3475.749 kb on + strand, within Psyr_2896 at 3475.749 kb on + strand, within Psyr_2896 at 3475.749 kb on + strand, within Psyr_2896 at 3475.750 kb on - strand, within Psyr_2896 at 3475.750 kb on - strand, within Psyr_2896 at 3475.750 kb on - strand, within Psyr_2896 at 3475.750 kb on - strand, within Psyr_2896 at 3475.750 kb on - strand, within Psyr_2896 at 3475.804 kb on - strand, within Psyr_2896 at 3475.836 kb on + strand, within Psyr_2896 at 3475.836 kb on + strand, within Psyr_2896 at 3475.836 kb on + strand, within Psyr_2896 at 3475.836 kb on + strand, within Psyr_2896 at 3475.836 kb on + strand, within Psyr_2896 at 3475.837 kb on - strand, within Psyr_2896 at 3475.837 kb on - strand, within Psyr_2896 at 3475.837 kb on - strand, within Psyr_2896 at 3475.837 kb on - strand, within Psyr_2896 at 3475.837 kb on - strand, within Psyr_2896 at 3475.927 kb on - strand, within Psyr_2896 at 3475.927 kb on - strand, within Psyr_2896 at 3475.998 kb on + strand, within Psyr_2896 at 3475.998 kb on + strand, within Psyr_2896 at 3475.998 kb on + strand, within Psyr_2896 at 3475.998 kb on + strand, within Psyr_2896 at 3475.999 kb on - strand, within Psyr_2896 at 3475.999 kb on - strand, within Psyr_2896 at 3476.035 kb on - strand, within Psyr_2896 at 3476.172 kb on + strand at 3476.172 kb on + strand at 3476.214 kb on + strand at 3476.242 kb on + strand at 3476.242 kb on + strand at 3476.242 kb on + strand at 3476.242 kb on + strand at 3476.242 kb on + strand at 3476.242 kb on + strand at 3476.242 kb on + strand at 3476.243 kb on - strand at 3476.243 kb on - strand at 3476.243 kb on - strand at 3476.341 kb on + strand at 3476.342 kb on - strand at 3476.342 kb on - strand at 3476.394 kb on + strand, within Psyr_2897 at 3476.405 kb on + strand, within Psyr_2897 at 3476.405 kb on + strand, within Psyr_2897 at 3476.406 kb on - strand, within Psyr_2897 at 3476.409 kb on + strand, within Psyr_2897 at 3476.409 kb on + strand, within Psyr_2897 at 3476.409 kb on + strand, within Psyr_2897 at 3476.409 kb on + strand, within Psyr_2897 at 3476.409 kb on + strand, within Psyr_2897 at 3476.409 kb on + strand, within Psyr_2897 at 3476.410 kb on - strand, within Psyr_2897 at 3476.410 kb on - strand, within Psyr_2897 at 3476.410 kb on - strand, within Psyr_2897 at 3476.410 kb on - strand, within Psyr_2897 at 3476.410 kb on - strand, within Psyr_2897 at 3476.410 kb on - strand, within Psyr_2897 at 3476.410 kb on - strand, within Psyr_2897 at 3476.410 kb on - strand, within Psyr_2897 at 3476.585 kb on + strand, within Psyr_2897 at 3476.585 kb on + strand, within Psyr_2897 at 3476.586 kb on - strand, within Psyr_2897 at 3476.654 kb on + strand, within Psyr_2897 at 3476.654 kb on + strand, within Psyr_2897 at 3476.655 kb on - strand, within Psyr_2897 at 3476.941 kb on + strand at 3476.941 kb on + strand at 3476.941 kb on + strand at 3476.942 kb on - strand at 3476.942 kb on - strand at 3476.942 kb on - strand at 3476.942 kb on - strand at 3476.942 kb on - strand at 3476.942 kb on - strand at 3476.942 kb on - strand at 3476.942 kb on - strand at 3476.949 kb on + strand at 3476.949 kb on + strand at 3476.949 kb on + strand at 3476.950 kb on - strand at 3476.979 kb on + strand at 3477.021 kb on + strand at 3477.021 kb on + strand at 3477.022 kb on - strand at 3477.049 kb on + strand at 3477.050 kb on - strand at 3477.050 kb on - strand at 3477.124 kb on - strand at 3477.124 kb on - strand at 3477.124 kb on - strand at 3477.130 kb on + strand at 3477.234 kb on + strand, within Psyr_2898 at 3477.260 kb on - strand, within Psyr_2898
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cantharidin 10 mM remove 3,473,545 + Psyr_2894 0.75 +0.6 3,473,546 - Psyr_2894 0.75 -0.3 3,473,656 + +0.4 3,473,656 + +0.7 3,473,657 - -0.4 3,473,657 - +0.7 3,474,508 - -0.1 3,474,508 - -2.3 3,474,733 + Psyr_2896 0.14 -0.2 3,474,793 - Psyr_2896 0.17 +0.5 3,474,863 + Psyr_2896 0.21 +0.0 3,474,964 - Psyr_2896 0.26 -0.4 3,474,964 - Psyr_2896 0.26 -1.4 3,474,996 + Psyr_2896 0.28 -0.0 3,475,119 + Psyr_2896 0.35 -0.4 3,475,405 - Psyr_2896 0.50 -0.2 3,475,448 + Psyr_2896 0.53 -1.6 3,475,448 + Psyr_2896 0.53 -0.8 3,475,449 - Psyr_2896 0.53 +0.2 3,475,449 - Psyr_2896 0.53 +0.2 3,475,449 - Psyr_2896 0.53 +0.5 3,475,736 + Psyr_2896 0.69 -0.5 3,475,736 + Psyr_2896 0.69 -0.6 3,475,736 + Psyr_2896 0.69 -0.3 3,475,736 + Psyr_2896 0.69 +0.9 3,475,736 + Psyr_2896 0.69 +0.2 3,475,736 + Psyr_2896 0.69 +0.0 3,475,736 + Psyr_2896 0.69 +0.5 3,475,736 + Psyr_2896 0.69 +0.2 3,475,736 + Psyr_2896 0.69 -0.3 3,475,737 - Psyr_2896 0.69 -0.8 3,475,737 - Psyr_2896 0.69 -1.1 3,475,737 - Psyr_2896 0.69 -0.4 3,475,737 - Psyr_2896 0.69 -0.1 3,475,737 - Psyr_2896 0.69 -0.0 3,475,737 - Psyr_2896 0.69 -0.8 3,475,749 + Psyr_2896 0.69 -2.1 3,475,749 + Psyr_2896 0.69 -0.8 3,475,749 + Psyr_2896 0.69 -1.1 3,475,749 + Psyr_2896 0.69 -0.6 3,475,749 + Psyr_2896 0.69 +1.0 3,475,750 - Psyr_2896 0.69 +0.8 3,475,750 - Psyr_2896 0.69 +0.6 3,475,750 - Psyr_2896 0.69 -0.5 3,475,750 - Psyr_2896 0.69 -0.8 3,475,750 - Psyr_2896 0.69 +0.3 3,475,804 - Psyr_2896 0.72 -1.2 3,475,836 + Psyr_2896 0.74 -0.7 3,475,836 + Psyr_2896 0.74 +0.8 3,475,836 + Psyr_2896 0.74 +0.9 3,475,836 + Psyr_2896 0.74 -1.1 3,475,836 + Psyr_2896 0.74 -0.5 3,475,837 - Psyr_2896 0.74 -0.4 3,475,837 - Psyr_2896 0.74 +0.9 3,475,837 - Psyr_2896 0.74 -0.5 3,475,837 - Psyr_2896 0.74 -0.7 3,475,837 - Psyr_2896 0.74 +0.0 3,475,927 - Psyr_2896 0.79 -0.1 3,475,927 - Psyr_2896 0.79 +0.6 3,475,998 + Psyr_2896 0.83 +0.4 3,475,998 + Psyr_2896 0.83 -0.7 3,475,998 + Psyr_2896 0.83 -0.6 3,475,998 + Psyr_2896 0.83 -0.8 3,475,999 - Psyr_2896 0.83 -0.2 3,475,999 - Psyr_2896 0.83 -1.5 3,476,035 - Psyr_2896 0.85 -0.2 3,476,172 + +1.6 3,476,172 + -0.1 3,476,214 + -0.8 3,476,242 + -0.4 3,476,242 + +1.9 3,476,242 + -1.1 3,476,242 + +0.6 3,476,242 + -0.4 3,476,242 + -0.5 3,476,242 + +0.6 3,476,243 - -1.1 3,476,243 - -0.5 3,476,243 - +1.2 3,476,341 + -0.8 3,476,342 - -1.6 3,476,342 - -1.4 3,476,394 + Psyr_2897 0.12 +0.7 3,476,405 + Psyr_2897 0.14 -0.4 3,476,405 + Psyr_2897 0.14 -0.9 3,476,406 - Psyr_2897 0.14 +1.6 3,476,409 + Psyr_2897 0.15 +0.2 3,476,409 + Psyr_2897 0.15 -1.1 3,476,409 + Psyr_2897 0.15 +1.8 3,476,409 + Psyr_2897 0.15 -1.6 3,476,409 + Psyr_2897 0.15 -2.9 3,476,409 + Psyr_2897 0.15 -0.2 3,476,410 - Psyr_2897 0.15 +0.8 3,476,410 - Psyr_2897 0.15 -1.8 3,476,410 - Psyr_2897 0.15 -0.2 3,476,410 - Psyr_2897 0.15 -0.6 3,476,410 - Psyr_2897 0.15 -1.8 3,476,410 - Psyr_2897 0.15 +0.2 3,476,410 - Psyr_2897 0.15 +1.2 3,476,410 - Psyr_2897 0.15 +0.2 3,476,585 + Psyr_2897 0.41 +0.8 3,476,585 + Psyr_2897 0.41 -0.6 3,476,586 - Psyr_2897 0.41 +0.0 3,476,654 + Psyr_2897 0.51 -2.0 3,476,654 + Psyr_2897 0.51 -2.4 3,476,655 - Psyr_2897 0.51 +0.9 3,476,941 + -0.5 3,476,941 + -0.2 3,476,941 + -0.8 3,476,942 - +0.2 3,476,942 - +1.2 3,476,942 - -0.6 3,476,942 - -0.3 3,476,942 - +2.8 3,476,942 - +1.2 3,476,942 - -0.6 3,476,942 - -0.9 3,476,949 + -1.0 3,476,949 + -0.7 3,476,949 + -0.6 3,476,950 - +0.6 3,476,979 + -0.7 3,477,021 + -1.1 3,477,021 + -2.3 3,477,022 - -1.0 3,477,049 + +0.8 3,477,050 - +0.2 3,477,050 - -0.7 3,477,124 - -1.3 3,477,124 - +1.4 3,477,124 - -0.8 3,477,130 + +0.1 3,477,234 + Psyr_2898 0.30 -1.8 3,477,260 - Psyr_2898 0.37 -0.2
Or see this region's nucleotide sequence