Experiment: KB with Cantharidin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2439 and Psyr_2440 are separated by 87 nucleotides Psyr_2440 and Psyr_2441 are separated by 240 nucleotides
Psyr_2439: Psyr_2439 - mannitol ABC transporter membrane protein / sorbitol ABC transporter membrane protein, at 2,823,940 to 2,824,872
_2439
Psyr_2440: Psyr_2440 - mannitol-binding protein / sorbitol-binding protein, at 2,824,960 to 2,826,282
_2440
Psyr_2441: Psyr_2441 - transcriptional regulator, AraC family, at 2,826,523 to 2,827,446
_2441
Position (kb)
2824
2825
2826
2827 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2824.004 kb on - strand at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.070 kb on + strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.071 kb on - strand, within Psyr_2439 at 2824.703 kb on + strand, within Psyr_2439 at 2824.721 kb on + strand, within Psyr_2439 at 2824.721 kb on + strand, within Psyr_2439 at 2824.721 kb on + strand, within Psyr_2439 at 2824.721 kb on + strand, within Psyr_2439 at 2824.722 kb on - strand, within Psyr_2439 at 2824.722 kb on - strand, within Psyr_2439 at 2824.866 kb on + strand at 2824.866 kb on + strand at 2824.962 kb on - strand at 2825.047 kb on + strand at 2825.048 kb on - strand at 2825.048 kb on - strand at 2825.048 kb on - strand at 2825.048 kb on - strand at 2825.048 kb on - strand at 2825.130 kb on - strand, within Psyr_2440 at 2825.130 kb on - strand, within Psyr_2440 at 2825.130 kb on - strand, within Psyr_2440 at 2825.130 kb on - strand, within Psyr_2440 at 2825.217 kb on - strand, within Psyr_2440 at 2825.228 kb on + strand, within Psyr_2440 at 2825.228 kb on + strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.229 kb on - strand, within Psyr_2440 at 2825.378 kb on + strand, within Psyr_2440 at 2825.421 kb on - strand, within Psyr_2440 at 2825.421 kb on - strand, within Psyr_2440 at 2825.421 kb on - strand, within Psyr_2440 at 2825.421 kb on - strand, within Psyr_2440 at 2825.587 kb on - strand, within Psyr_2440 at 2825.595 kb on - strand, within Psyr_2440 at 2825.595 kb on - strand, within Psyr_2440 at 2825.595 kb on - strand, within Psyr_2440 at 2825.669 kb on + strand, within Psyr_2440 at 2825.741 kb on + strand, within Psyr_2440 at 2825.799 kb on + strand, within Psyr_2440 at 2825.852 kb on + strand, within Psyr_2440 at 2825.852 kb on + strand, within Psyr_2440 at 2825.852 kb on + strand, within Psyr_2440 at 2825.852 kb on + strand, within Psyr_2440 at 2825.853 kb on - strand, within Psyr_2440 at 2825.874 kb on - strand, within Psyr_2440 at 2825.874 kb on - strand, within Psyr_2440 at 2825.874 kb on - strand, within Psyr_2440 at 2825.921 kb on - strand, within Psyr_2440 at 2825.948 kb on + strand, within Psyr_2440 at 2825.948 kb on + strand, within Psyr_2440 at 2825.949 kb on - strand, within Psyr_2440 at 2825.949 kb on - strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.011 kb on + strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.012 kb on - strand, within Psyr_2440 at 2826.326 kb on + strand at 2826.370 kb on + strand at 2826.370 kb on + strand at 2826.371 kb on - strand at 2826.625 kb on - strand, within Psyr_2441 at 2826.703 kb on - strand, within Psyr_2441 at 2826.767 kb on + strand, within Psyr_2441 at 2826.768 kb on - strand, within Psyr_2441 at 2826.837 kb on + strand, within Psyr_2441 at 2826.838 kb on - strand, within Psyr_2441 at 2827.194 kb on + strand, within Psyr_2441 at 2827.194 kb on + strand, within Psyr_2441 at 2827.194 kb on + strand, within Psyr_2441 at 2827.250 kb on + strand, within Psyr_2441 at 2827.250 kb on + strand, within Psyr_2441
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cantharidin 10 mM remove 2,824,004 - -2.5 2,824,070 + Psyr_2439 0.14 -2.0 2,824,070 + Psyr_2439 0.14 +0.0 2,824,070 + Psyr_2439 0.14 +0.8 2,824,070 + Psyr_2439 0.14 -0.4 2,824,070 + Psyr_2439 0.14 +0.2 2,824,070 + Psyr_2439 0.14 +0.9 2,824,071 - Psyr_2439 0.14 +1.6 2,824,071 - Psyr_2439 0.14 +2.2 2,824,071 - Psyr_2439 0.14 -0.1 2,824,071 - Psyr_2439 0.14 -0.9 2,824,071 - Psyr_2439 0.14 -2.1 2,824,703 + Psyr_2439 0.82 +0.9 2,824,721 + Psyr_2439 0.84 +0.4 2,824,721 + Psyr_2439 0.84 -0.3 2,824,721 + Psyr_2439 0.84 +0.1 2,824,721 + Psyr_2439 0.84 -0.4 2,824,722 - Psyr_2439 0.84 +0.9 2,824,722 - Psyr_2439 0.84 +0.6 2,824,866 + +0.9 2,824,866 + +1.2 2,824,962 - +0.2 2,825,047 + +1.0 2,825,048 - +0.2 2,825,048 - +0.5 2,825,048 - +0.1 2,825,048 - -1.0 2,825,048 - +0.2 2,825,130 - Psyr_2440 0.13 -1.0 2,825,130 - Psyr_2440 0.13 +1.7 2,825,130 - Psyr_2440 0.13 +1.2 2,825,130 - Psyr_2440 0.13 -0.4 2,825,217 - Psyr_2440 0.19 +0.4 2,825,228 + Psyr_2440 0.20 -0.6 2,825,228 + Psyr_2440 0.20 -1.8 2,825,229 - Psyr_2440 0.20 +1.2 2,825,229 - Psyr_2440 0.20 -0.8 2,825,229 - Psyr_2440 0.20 -0.4 2,825,229 - Psyr_2440 0.20 -1.9 2,825,229 - Psyr_2440 0.20 +0.2 2,825,229 - Psyr_2440 0.20 +0.5 2,825,378 + Psyr_2440 0.32 -0.5 2,825,421 - Psyr_2440 0.35 +1.8 2,825,421 - Psyr_2440 0.35 -0.1 2,825,421 - Psyr_2440 0.35 -0.5 2,825,421 - Psyr_2440 0.35 -0.4 2,825,587 - Psyr_2440 0.47 +0.5 2,825,595 - Psyr_2440 0.48 -0.4 2,825,595 - Psyr_2440 0.48 +0.2 2,825,595 - Psyr_2440 0.48 -0.1 2,825,669 + Psyr_2440 0.54 -0.1 2,825,741 + Psyr_2440 0.59 -0.5 2,825,799 + Psyr_2440 0.63 +0.2 2,825,852 + Psyr_2440 0.67 -0.4 2,825,852 + Psyr_2440 0.67 -0.2 2,825,852 + Psyr_2440 0.67 +2.5 2,825,852 + Psyr_2440 0.67 -0.3 2,825,853 - Psyr_2440 0.67 -0.3 2,825,874 - Psyr_2440 0.69 -0.3 2,825,874 - Psyr_2440 0.69 -0.6 2,825,874 - Psyr_2440 0.69 -0.4 2,825,921 - Psyr_2440 0.73 -1.2 2,825,948 + Psyr_2440 0.75 +0.3 2,825,948 + Psyr_2440 0.75 -0.2 2,825,949 - Psyr_2440 0.75 +0.8 2,825,949 - Psyr_2440 0.75 -2.5 2,826,011 + Psyr_2440 0.79 -0.8 2,826,011 + Psyr_2440 0.79 +1.0 2,826,011 + Psyr_2440 0.79 -1.4 2,826,011 + Psyr_2440 0.79 +0.3 2,826,011 + Psyr_2440 0.79 +2.8 2,826,011 + Psyr_2440 0.79 -0.6 2,826,011 + Psyr_2440 0.79 +0.4 2,826,011 + Psyr_2440 0.79 -0.9 2,826,012 - Psyr_2440 0.80 +0.2 2,826,012 - Psyr_2440 0.80 -2.0 2,826,012 - Psyr_2440 0.80 -0.3 2,826,012 - Psyr_2440 0.80 +1.2 2,826,012 - Psyr_2440 0.80 -0.8 2,826,012 - Psyr_2440 0.80 -0.2 2,826,012 - Psyr_2440 0.80 -0.4 2,826,326 + -1.8 2,826,370 + +0.9 2,826,370 + +0.0 2,826,371 - -0.2 2,826,625 - Psyr_2441 0.11 -1.3 2,826,703 - Psyr_2441 0.19 +1.5 2,826,767 + Psyr_2441 0.26 -0.1 2,826,768 - Psyr_2441 0.27 -1.8 2,826,837 + Psyr_2441 0.34 +0.2 2,826,838 - Psyr_2441 0.34 +0.2 2,827,194 + Psyr_2441 0.73 -0.1 2,827,194 + Psyr_2441 0.73 -0.1 2,827,194 + Psyr_2441 0.73 +2.0 2,827,250 + Psyr_2441 0.79 +0.4 2,827,250 + Psyr_2441 0.79 +0.2
Or see this region's nucleotide sequence