Experiment: KB with Cantharidin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2193 and Psyr_2194 overlap by 4 nucleotides Psyr_2194 and Psyr_2195 are separated by 225 nucleotides
Psyr_2193: Psyr_2193 - Secretion protein HlyD, at 2,548,506 to 2,549,609
_2193
Psyr_2194: Psyr_2194 - Acriflavin resistance protein, at 2,549,606 to 2,552,725
_2194
Psyr_2195: Psyr_2195 - Urease accessory protein UreD, at 2,552,951 to 2,553,808
_2195
Position (kb)
2549
2550
2551
2552
2553 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2549.191 kb on - strand, within Psyr_2193 at 2549.191 kb on - strand, within Psyr_2193 at 2549.262 kb on - strand, within Psyr_2193 at 2549.277 kb on + strand, within Psyr_2193 at 2549.277 kb on + strand at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.458 kb on + strand, within Psyr_2193 at 2549.545 kb on + strand at 2549.545 kb on + strand at 2549.545 kb on + strand at 2549.586 kb on - strand at 2549.674 kb on + strand at 2549.761 kb on + strand at 2549.762 kb on - strand at 2549.867 kb on + strand at 2549.867 kb on + strand at 2549.867 kb on + strand at 2549.945 kb on + strand, within Psyr_2194 at 2549.946 kb on - strand, within Psyr_2194 at 2550.775 kb on + strand, within Psyr_2194 at 2550.775 kb on + strand, within Psyr_2194 at 2550.775 kb on + strand, within Psyr_2194 at 2550.775 kb on + strand, within Psyr_2194 at 2550.778 kb on + strand, within Psyr_2194 at 2550.778 kb on + strand, within Psyr_2194 at 2550.779 kb on - strand, within Psyr_2194 at 2550.893 kb on + strand, within Psyr_2194 at 2550.893 kb on + strand, within Psyr_2194 at 2551.067 kb on + strand, within Psyr_2194 at 2551.067 kb on + strand, within Psyr_2194 at 2551.068 kb on - strand, within Psyr_2194 at 2551.128 kb on - strand, within Psyr_2194 at 2551.142 kb on + strand, within Psyr_2194 at 2551.143 kb on - strand, within Psyr_2194 at 2551.143 kb on - strand, within Psyr_2194 at 2551.288 kb on - strand, within Psyr_2194 at 2551.311 kb on - strand, within Psyr_2194 at 2551.400 kb on + strand, within Psyr_2194 at 2551.401 kb on - strand, within Psyr_2194 at 2551.401 kb on - strand, within Psyr_2194 at 2551.427 kb on + strand, within Psyr_2194 at 2551.479 kb on + strand, within Psyr_2194 at 2551.480 kb on - strand, within Psyr_2194 at 2551.595 kb on + strand, within Psyr_2194 at 2551.596 kb on - strand, within Psyr_2194 at 2552.160 kb on + strand, within Psyr_2194 at 2552.311 kb on - strand, within Psyr_2194 at 2552.395 kb on + strand, within Psyr_2194 at 2552.395 kb on + strand, within Psyr_2194 at 2552.396 kb on - strand, within Psyr_2194 at 2552.475 kb on + strand at 2552.630 kb on + strand at 2552.630 kb on + strand at 2552.630 kb on + strand at 2552.631 kb on - strand at 2552.631 kb on - strand at 2552.631 kb on - strand at 2552.836 kb on - strand at 2552.838 kb on + strand at 2552.838 kb on + strand at 2552.907 kb on + strand at 2553.103 kb on - strand, within Psyr_2195 at 2553.346 kb on + strand, within Psyr_2195 at 2553.346 kb on + strand, within Psyr_2195 at 2553.346 kb on + strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.398 kb on + strand, within Psyr_2195 at 2553.399 kb on - strand, within Psyr_2195 at 2553.399 kb on - strand, within Psyr_2195 at 2553.457 kb on - strand, within Psyr_2195 at 2553.457 kb on - strand, within Psyr_2195 at 2553.637 kb on - strand, within Psyr_2195 at 2553.723 kb on + strand at 2553.723 kb on + strand at 2553.723 kb on + strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cantharidin 10 mM remove 2,549,191 - Psyr_2193 0.62 -0.4 2,549,191 - Psyr_2193 0.62 +0.6 2,549,262 - Psyr_2193 0.68 +1.5 2,549,277 + Psyr_2193 0.70 +0.7 2,549,277 + +2.2 2,549,278 - Psyr_2193 0.70 +0.6 2,549,278 - Psyr_2193 0.70 +0.5 2,549,278 - Psyr_2193 0.70 +0.5 2,549,278 - Psyr_2193 0.70 -0.1 2,549,278 - Psyr_2193 0.70 +0.1 2,549,278 - Psyr_2193 0.70 -2.0 2,549,458 + Psyr_2193 0.86 -0.2 2,549,545 + +0.2 2,549,545 + -0.5 2,549,545 + +0.5 2,549,586 - +0.8 2,549,674 + -2.0 2,549,761 + +0.1 2,549,762 - -2.3 2,549,867 + -0.5 2,549,867 + +0.2 2,549,867 + +1.2 2,549,945 + Psyr_2194 0.11 +0.6 2,549,946 - Psyr_2194 0.11 -2.8 2,550,775 + Psyr_2194 0.37 +1.2 2,550,775 + Psyr_2194 0.37 +1.0 2,550,775 + Psyr_2194 0.37 -0.4 2,550,775 + Psyr_2194 0.37 +0.3 2,550,778 + Psyr_2194 0.38 -0.0 2,550,778 + Psyr_2194 0.38 +0.1 2,550,779 - Psyr_2194 0.38 -0.8 2,550,893 + Psyr_2194 0.41 +1.2 2,550,893 + Psyr_2194 0.41 -0.5 2,551,067 + Psyr_2194 0.47 -1.0 2,551,067 + Psyr_2194 0.47 -1.5 2,551,068 - Psyr_2194 0.47 -1.0 2,551,128 - Psyr_2194 0.49 -1.4 2,551,142 + Psyr_2194 0.49 -0.0 2,551,143 - Psyr_2194 0.49 +0.7 2,551,143 - Psyr_2194 0.49 -0.5 2,551,288 - Psyr_2194 0.54 -1.0 2,551,311 - Psyr_2194 0.55 -0.6 2,551,400 + Psyr_2194 0.57 +0.9 2,551,401 - Psyr_2194 0.58 -2.3 2,551,401 - Psyr_2194 0.58 -0.1 2,551,427 + Psyr_2194 0.58 +1.2 2,551,479 + Psyr_2194 0.60 +0.4 2,551,480 - Psyr_2194 0.60 -2.1 2,551,595 + Psyr_2194 0.64 +0.6 2,551,596 - Psyr_2194 0.64 +2.2 2,552,160 + Psyr_2194 0.82 -1.1 2,552,311 - Psyr_2194 0.87 -0.8 2,552,395 + Psyr_2194 0.89 -1.7 2,552,395 + Psyr_2194 0.89 -0.3 2,552,396 - Psyr_2194 0.89 -0.2 2,552,475 + +1.3 2,552,630 + -1.0 2,552,630 + +0.7 2,552,630 + -1.5 2,552,631 - -1.1 2,552,631 - +0.2 2,552,631 - -0.5 2,552,836 - -2.0 2,552,838 + +0.2 2,552,838 + -1.5 2,552,907 + +1.4 2,553,103 - Psyr_2195 0.18 -0.4 2,553,346 + Psyr_2195 0.46 -0.7 2,553,346 + Psyr_2195 0.46 -0.2 2,553,346 + Psyr_2195 0.46 +0.3 2,553,347 - Psyr_2195 0.46 +0.7 2,553,347 - Psyr_2195 0.46 +0.5 2,553,347 - Psyr_2195 0.46 +0.3 2,553,347 - Psyr_2195 0.46 +1.2 2,553,347 - Psyr_2195 0.46 -0.4 2,553,347 - Psyr_2195 0.46 +0.1 2,553,398 + Psyr_2195 0.52 +0.4 2,553,399 - Psyr_2195 0.52 -1.4 2,553,399 - Psyr_2195 0.52 -1.8 2,553,457 - Psyr_2195 0.59 -2.3 2,553,457 - Psyr_2195 0.59 +0.5 2,553,637 - Psyr_2195 0.80 +0.4 2,553,723 + +0.3 2,553,723 + +0.9 2,553,723 + -0.4 2,553,724 - +1.2 2,553,724 - +0.7 2,553,724 - -0.4 2,553,724 - -1.0 2,553,724 - -1.8
Or see this region's nucleotide sequence