Experiment: KB with Cantharidin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1979 and Psyr_1980 are separated by 129 nucleotides Psyr_1980 and Psyr_1981 are separated by 67 nucleotides
Psyr_1979: Psyr_1979 - tRNA-U20a,U20b-dihydrouridine synthase, at 2,297,844 to 2,298,809
_1979
Psyr_1980: Psyr_1980 - Heat shock protein Hsp20, at 2,298,939 to 2,299,382
_1980
Psyr_1981: Psyr_1981 - PAS:GGDEF, at 2,299,450 to 2,301,642
_1981
Position (kb)
2298
2299
2300 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2297.977 kb on - strand, within Psyr_1979 at 2297.977 kb on - strand, within Psyr_1979 at 2297.977 kb on - strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.982 kb on + strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.983 kb on - strand, within Psyr_1979 at 2297.988 kb on + strand, within Psyr_1979 at 2297.988 kb on + strand, within Psyr_1979 at 2297.988 kb on + strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2297.989 kb on - strand, within Psyr_1979 at 2298.029 kb on + strand, within Psyr_1979 at 2298.244 kb on + strand, within Psyr_1979 at 2298.369 kb on + strand, within Psyr_1979 at 2298.369 kb on + strand, within Psyr_1979 at 2298.731 kb on + strand at 2298.731 kb on + strand at 2298.731 kb on + strand at 2298.732 kb on - strand at 2298.732 kb on - strand at 2298.769 kb on + strand at 2298.770 kb on - strand at 2298.770 kb on - strand at 2298.870 kb on + strand at 2298.871 kb on - strand at 2298.908 kb on - strand at 2298.944 kb on + strand at 2298.945 kb on - strand at 2299.053 kb on + strand, within Psyr_1980 at 2299.053 kb on + strand, within Psyr_1980 at 2299.054 kb on - strand, within Psyr_1980 at 2299.200 kb on + strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.259 kb on + strand, within Psyr_1980 at 2299.413 kb on + strand at 2299.414 kb on - strand at 2299.538 kb on + strand at 2299.547 kb on + strand at 2299.547 kb on + strand at 2299.547 kb on - strand at 2299.548 kb on - strand at 2299.548 kb on - strand at 2299.548 kb on - strand at 2299.553 kb on + strand at 2299.554 kb on - strand at 2299.554 kb on - strand at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.743 kb on - strand, within Psyr_1981 at 2299.743 kb on - strand, within Psyr_1981 at 2299.851 kb on + strand, within Psyr_1981 at 2299.852 kb on - strand, within Psyr_1981 at 2299.960 kb on - strand, within Psyr_1981 at 2300.043 kb on + strand, within Psyr_1981 at 2300.043 kb on + strand, within Psyr_1981 at 2300.044 kb on - strand, within Psyr_1981 at 2300.083 kb on + strand, within Psyr_1981 at 2300.083 kb on + strand, within Psyr_1981 at 2300.083 kb on + strand, within Psyr_1981 at 2300.084 kb on - strand, within Psyr_1981 at 2300.084 kb on - strand, within Psyr_1981 at 2300.084 kb on - strand, within Psyr_1981 at 2300.117 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cantharidin 10 mM remove 2,297,977 - Psyr_1979 0.14 +1.4 2,297,977 - Psyr_1979 0.14 +1.3 2,297,977 - Psyr_1979 0.14 -1.7 2,297,982 + Psyr_1979 0.14 -1.2 2,297,982 + Psyr_1979 0.14 -0.5 2,297,982 + Psyr_1979 0.14 -0.3 2,297,982 + Psyr_1979 0.14 +1.3 2,297,982 + Psyr_1979 0.14 +0.4 2,297,982 + Psyr_1979 0.14 -0.2 2,297,983 - Psyr_1979 0.14 -1.1 2,297,983 - Psyr_1979 0.14 -1.3 2,297,983 - Psyr_1979 0.14 -0.3 2,297,983 - Psyr_1979 0.14 -0.4 2,297,983 - Psyr_1979 0.14 -0.9 2,297,988 + Psyr_1979 0.15 -1.1 2,297,988 + Psyr_1979 0.15 +1.1 2,297,988 + Psyr_1979 0.15 -1.0 2,297,989 - Psyr_1979 0.15 +1.3 2,297,989 - Psyr_1979 0.15 -0.0 2,297,989 - Psyr_1979 0.15 +1.0 2,297,989 - Psyr_1979 0.15 -0.1 2,297,989 - Psyr_1979 0.15 +0.3 2,298,029 + Psyr_1979 0.19 +0.1 2,298,244 + Psyr_1979 0.41 +2.1 2,298,369 + Psyr_1979 0.54 +2.5 2,298,369 + Psyr_1979 0.54 +0.1 2,298,731 + +0.1 2,298,731 + -0.6 2,298,731 + -0.6 2,298,732 - +0.1 2,298,732 - +0.6 2,298,769 + -2.3 2,298,770 - -0.1 2,298,770 - -0.7 2,298,870 + -0.6 2,298,871 - -0.8 2,298,908 - -0.2 2,298,944 + +0.3 2,298,945 - +1.8 2,299,053 + Psyr_1980 0.26 -1.2 2,299,053 + Psyr_1980 0.26 +0.9 2,299,054 - Psyr_1980 0.26 -1.0 2,299,200 + Psyr_1980 0.59 -1.0 2,299,201 - Psyr_1980 0.59 -1.2 2,299,201 - Psyr_1980 0.59 -0.9 2,299,201 - Psyr_1980 0.59 -0.7 2,299,259 + Psyr_1980 0.72 +0.6 2,299,413 + -0.6 2,299,414 - -1.8 2,299,538 + +0.9 2,299,547 + -0.2 2,299,547 + -1.1 2,299,547 - +0.2 2,299,548 - +0.3 2,299,548 - -1.0 2,299,548 - -0.8 2,299,553 + -0.0 2,299,554 - +0.8 2,299,554 - +1.0 2,299,742 + Psyr_1981 0.13 -0.8 2,299,742 + Psyr_1981 0.13 -0.1 2,299,742 + Psyr_1981 0.13 -1.2 2,299,742 + Psyr_1981 0.13 +0.2 2,299,742 + Psyr_1981 0.13 -0.2 2,299,743 - Psyr_1981 0.13 -0.4 2,299,743 - Psyr_1981 0.13 -0.5 2,299,851 + Psyr_1981 0.18 +0.0 2,299,852 - Psyr_1981 0.18 -1.7 2,299,960 - Psyr_1981 0.23 +1.3 2,300,043 + Psyr_1981 0.27 +0.3 2,300,043 + Psyr_1981 0.27 -0.3 2,300,044 - Psyr_1981 0.27 -0.1 2,300,083 + Psyr_1981 0.29 -0.2 2,300,083 + Psyr_1981 0.29 +0.1 2,300,083 + Psyr_1981 0.29 +0.5 2,300,084 - Psyr_1981 0.29 +0.8 2,300,084 - Psyr_1981 0.29 -0.5 2,300,084 - Psyr_1981 0.29 +0.5 2,300,117 + Psyr_1981 0.30 +0.1 2,300,162 + Psyr_1981 0.32 -0.1 2,300,162 + Psyr_1981 0.32 +1.1 2,300,162 + Psyr_1981 0.32 -2.2 2,300,162 + Psyr_1981 0.32 +0.6 2,300,162 + Psyr_1981 0.32 -0.4 2,300,163 - Psyr_1981 0.33 +1.4 2,300,163 - Psyr_1981 0.33 +0.0 2,300,163 - Psyr_1981 0.33 -0.4 2,300,163 - Psyr_1981 0.33 +0.4 2,300,163 - Psyr_1981 0.33 +0.1 2,300,163 - Psyr_1981 0.33 +0.2
Or see this region's nucleotide sequence