Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22335

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22330 and BWI76_RS22335 overlap by 4 nucleotidesBWI76_RS22335 and BWI76_RS22340 are separated by 82 nucleotidesBWI76_RS22340 and BWI76_RS22345 are separated by 208 nucleotides BWI76_RS22330: BWI76_RS22330 - formate hydrogenlyase subunit 3, at 4,654,804 to 4,656,630 _RS22330 BWI76_RS22335: BWI76_RS22335 - 4Fe-4S ferredoxin, at 4,656,627 to 4,657,235 _RS22335 BWI76_RS22340: BWI76_RS22340 - formate hydrogenlyase regulatory protein HycA, at 4,657,318 to 4,657,767 _RS22340 BWI76_RS22345: BWI76_RS22345 - hydrogenase maturation nickel metallochaperone HypA, at 4,657,976 to 4,658,326 _RS22345 Position (kb) 4656 4657 4658Strain fitness (log2 ratio) -1 0 1at 4655.682 kb on - strand, within BWI76_RS22330at 4655.909 kb on + strand, within BWI76_RS22330at 4656.349 kb on - strand, within BWI76_RS22330at 4656.400 kb on + strand, within BWI76_RS22330at 4656.438 kb on + strand, within BWI76_RS22330at 4656.474 kb on - strandat 4656.770 kb on - strand, within BWI76_RS22335at 4656.836 kb on + strand, within BWI76_RS22335at 4656.849 kb on - strand, within BWI76_RS22335at 4656.883 kb on + strand, within BWI76_RS22335at 4656.921 kb on - strand, within BWI76_RS22335at 4657.292 kb on - strandat 4657.609 kb on - strand, within BWI76_RS22340at 4657.659 kb on + strand, within BWI76_RS22340at 4657.659 kb on + strand, within BWI76_RS22340at 4657.667 kb on - strand, within BWI76_RS22340at 4657.671 kb on + strand, within BWI76_RS22340at 4657.783 kb on + strandat 4657.783 kb on + strandat 4657.989 kb on + strandat 4657.989 kb on + strandat 4657.997 kb on - strandat 4658.045 kb on + strand, within BWI76_RS22345at 4658.102 kb on + strand, within BWI76_RS22345at 4658.151 kb on + strand, within BWI76_RS22345at 4658.161 kb on + strand, within BWI76_RS22345at 4658.174 kb on - strand, within BWI76_RS22345

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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4,655,682 - BWI76_RS22330 0.48 +0.4
4,655,909 + BWI76_RS22330 0.60 -0.3
4,656,349 - BWI76_RS22330 0.85 -0.1
4,656,400 + BWI76_RS22330 0.87 +0.8
4,656,438 + BWI76_RS22330 0.89 -0.0
4,656,474 - -0.0
4,656,770 - BWI76_RS22335 0.23 +0.3
4,656,836 + BWI76_RS22335 0.34 +0.7
4,656,849 - BWI76_RS22335 0.36 -0.3
4,656,883 + BWI76_RS22335 0.42 -0.8
4,656,921 - BWI76_RS22335 0.48 -0.1
4,657,292 - -0.8
4,657,609 - BWI76_RS22340 0.65 -0.0
4,657,659 + BWI76_RS22340 0.76 -0.0
4,657,659 + BWI76_RS22340 0.76 +0.6
4,657,667 - BWI76_RS22340 0.78 -1.1
4,657,671 + BWI76_RS22340 0.78 -0.5
4,657,783 + -0.2
4,657,783 + +0.2
4,657,989 + -0.0
4,657,989 + +0.2
4,657,997 - +0.1
4,658,045 + BWI76_RS22345 0.20 +0.4
4,658,102 + BWI76_RS22345 0.36 -0.7
4,658,151 + BWI76_RS22345 0.50 +0.7
4,658,161 + BWI76_RS22345 0.53 +0.1
4,658,174 - BWI76_RS22345 0.56 +0.6

Or see this region's nucleotide sequence