Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22320

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22310 and BWI76_RS22315 overlap by 1 nucleotidesBWI76_RS22315 and BWI76_RS22320 are separated by 9 nucleotidesBWI76_RS22320 and BWI76_RS22325 are separated by 17 nucleotidesBWI76_RS22325 and BWI76_RS22330 are separated by 2 nucleotides BWI76_RS22310: BWI76_RS22310 - hydrogenase 3 small subunit, at 4,650,832 to 4,651,599 _RS22310 BWI76_RS22315: BWI76_RS22315 - formate hydrogenlyase complex iron-sulfur subunit, at 4,651,599 to 4,652,141 _RS22315 BWI76_RS22320: BWI76_RS22320 - hydrogenase 3 large subunit, at 4,652,151 to 4,653,860 _RS22320 BWI76_RS22325: BWI76_RS22325 - hydrogenase 3 membrane subunit, at 4,653,878 to 4,654,801 _RS22325 BWI76_RS22330: BWI76_RS22330 - formate hydrogenlyase subunit 3, at 4,654,804 to 4,656,630 _RS22330 Position (kb) 4652 4653 4654Strain fitness (log2 ratio) -1 0 1at 4651.316 kb on + strand, within BWI76_RS22310at 4651.334 kb on - strand, within BWI76_RS22310at 4651.414 kb on - strand, within BWI76_RS22310at 4651.414 kb on - strand, within BWI76_RS22310at 4651.518 kb on + strand, within BWI76_RS22310at 4651.620 kb on - strandat 4651.646 kb on + strandat 4651.656 kb on + strand, within BWI76_RS22315at 4651.664 kb on - strand, within BWI76_RS22315at 4651.664 kb on - strand, within BWI76_RS22315at 4652.005 kb on - strand, within BWI76_RS22315at 4652.078 kb on + strand, within BWI76_RS22315at 4652.102 kb on - strandat 4652.190 kb on + strandat 4652.212 kb on + strandat 4652.234 kb on + strandat 4652.257 kb on + strandat 4652.257 kb on + strandat 4652.265 kb on - strandat 4652.281 kb on - strandat 4652.309 kb on + strandat 4652.392 kb on - strand, within BWI76_RS22320at 4652.465 kb on + strand, within BWI76_RS22320at 4652.472 kb on + strand, within BWI76_RS22320at 4652.473 kb on - strand, within BWI76_RS22320at 4652.542 kb on + strand, within BWI76_RS22320at 4652.550 kb on - strand, within BWI76_RS22320at 4652.550 kb on - strand, within BWI76_RS22320at 4652.597 kb on - strand, within BWI76_RS22320at 4652.794 kb on - strand, within BWI76_RS22320at 4652.884 kb on - strand, within BWI76_RS22320at 4652.932 kb on - strand, within BWI76_RS22320at 4653.168 kb on + strand, within BWI76_RS22320at 4653.222 kb on - strand, within BWI76_RS22320at 4653.289 kb on + strand, within BWI76_RS22320at 4653.314 kb on + strand, within BWI76_RS22320at 4653.327 kb on - strand, within BWI76_RS22320at 4653.353 kb on + strand, within BWI76_RS22320at 4653.581 kb on + strand, within BWI76_RS22320at 4653.607 kb on + strand, within BWI76_RS22320at 4653.779 kb on + strandat 4653.956 kb on - strandat 4654.110 kb on + strand, within BWI76_RS22325at 4654.128 kb on - strand, within BWI76_RS22325at 4654.312 kb on - strand, within BWI76_RS22325at 4654.328 kb on + strand, within BWI76_RS22325at 4654.367 kb on - strand, within BWI76_RS22325at 4654.576 kb on + strand, within BWI76_RS22325

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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4,651,316 + BWI76_RS22310 0.63 -0.2
4,651,334 - BWI76_RS22310 0.65 -1.2
4,651,414 - BWI76_RS22310 0.76 +0.2
4,651,414 - BWI76_RS22310 0.76 +0.2
4,651,518 + BWI76_RS22310 0.89 +0.1
4,651,620 - -0.7
4,651,646 + -0.8
4,651,656 + BWI76_RS22315 0.10 -0.9
4,651,664 - BWI76_RS22315 0.12 +0.8
4,651,664 - BWI76_RS22315 0.12 -0.9
4,652,005 - BWI76_RS22315 0.75 -0.7
4,652,078 + BWI76_RS22315 0.88 +0.3
4,652,102 - +1.6
4,652,190 + +1.0
4,652,212 + +0.1
4,652,234 + +0.3
4,652,257 + -0.3
4,652,257 + +0.8
4,652,265 - -0.2
4,652,281 - +0.3
4,652,309 + +0.1
4,652,392 - BWI76_RS22320 0.14 -0.2
4,652,465 + BWI76_RS22320 0.18 -0.6
4,652,472 + BWI76_RS22320 0.19 -0.6
4,652,473 - BWI76_RS22320 0.19 -0.3
4,652,542 + BWI76_RS22320 0.23 -0.0
4,652,550 - BWI76_RS22320 0.23 -0.9
4,652,550 - BWI76_RS22320 0.23 -0.0
4,652,597 - BWI76_RS22320 0.26 +0.7
4,652,794 - BWI76_RS22320 0.38 +0.4
4,652,884 - BWI76_RS22320 0.43 -0.4
4,652,932 - BWI76_RS22320 0.46 +0.8
4,653,168 + BWI76_RS22320 0.59 +0.2
4,653,222 - BWI76_RS22320 0.63 +1.1
4,653,289 + BWI76_RS22320 0.67 +0.4
4,653,314 + BWI76_RS22320 0.68 +0.3
4,653,327 - BWI76_RS22320 0.69 -0.2
4,653,353 + BWI76_RS22320 0.70 +0.2
4,653,581 + BWI76_RS22320 0.84 +0.4
4,653,607 + BWI76_RS22320 0.85 -0.2
4,653,779 + -0.5
4,653,956 - -0.0
4,654,110 + BWI76_RS22325 0.25 -0.4
4,654,128 - BWI76_RS22325 0.27 -0.6
4,654,312 - BWI76_RS22325 0.47 -0.8
4,654,328 + BWI76_RS22325 0.49 +1.0
4,654,367 - BWI76_RS22325 0.53 +0.0
4,654,576 + BWI76_RS22325 0.76 -0.2

Or see this region's nucleotide sequence