Experiment: 2'-Deoxycytidine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22300 and BWI76_RS22305 overlap by 8 nucleotides BWI76_RS22305 and BWI76_RS22310 overlap by 4 nucleotides BWI76_RS22310 and BWI76_RS22315 overlap by 1 nucleotides BWI76_RS22315 and BWI76_RS22320 are separated by 9 nucleotides
BWI76_RS22300: BWI76_RS22300 - hydrogenase 3 maturation endopeptidase HyCI, at 4,649,947 to 4,650,432
_RS22300
BWI76_RS22305: BWI76_RS22305 - formate hydrogenlyase maturation protein HycH, at 4,650,425 to 4,650,835
_RS22305
BWI76_RS22310: BWI76_RS22310 - hydrogenase 3 small subunit, at 4,650,832 to 4,651,599
_RS22310
BWI76_RS22315: BWI76_RS22315 - formate hydrogenlyase complex iron-sulfur subunit, at 4,651,599 to 4,652,141
_RS22315
BWI76_RS22320: BWI76_RS22320 - hydrogenase 3 large subunit, at 4,652,151 to 4,653,860
_RS22320
Position (kb) 4650
4651
4652 Strain fitness (log2 ratio) -1
0
1 at 4649.995 kb on + strand at 4650.366 kb on - strand, within BWI76_RS22300 at 4650.494 kb on - strand, within BWI76_RS22305 at 4650.990 kb on + strand, within BWI76_RS22310 at 4651.316 kb on + strand, within BWI76_RS22310 at 4651.334 kb on - strand, within BWI76_RS22310 at 4651.414 kb on - strand, within BWI76_RS22310 at 4651.414 kb on - strand, within BWI76_RS22310 at 4651.518 kb on + strand, within BWI76_RS22310 at 4651.620 kb on - strand at 4651.646 kb on + strand at 4651.656 kb on + strand, within BWI76_RS22315 at 4651.664 kb on - strand, within BWI76_RS22315 at 4651.664 kb on - strand, within BWI76_RS22315 at 4652.005 kb on - strand, within BWI76_RS22315 at 4652.078 kb on + strand, within BWI76_RS22315 at 4652.102 kb on - strand at 4652.190 kb on + strand at 4652.212 kb on + strand at 4652.234 kb on + strand at 4652.257 kb on + strand at 4652.257 kb on + strand at 4652.265 kb on - strand at 4652.281 kb on - strand at 4652.309 kb on + strand at 4652.392 kb on - strand, within BWI76_RS22320 at 4652.465 kb on + strand, within BWI76_RS22320 at 4652.472 kb on + strand, within BWI76_RS22320 at 4652.473 kb on - strand, within BWI76_RS22320 at 4652.542 kb on + strand, within BWI76_RS22320 at 4652.550 kb on - strand, within BWI76_RS22320 at 4652.550 kb on - strand, within BWI76_RS22320 at 4652.597 kb on - strand, within BWI76_RS22320
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C) remove 4,649,995 + +0.5 4,650,366 - BWI76_RS22300 0.86 -0.1 4,650,494 - BWI76_RS22305 0.17 -0.2 4,650,990 + BWI76_RS22310 0.21 +0.2 4,651,316 + BWI76_RS22310 0.63 -0.2 4,651,334 - BWI76_RS22310 0.65 -1.2 4,651,414 - BWI76_RS22310 0.76 +0.2 4,651,414 - BWI76_RS22310 0.76 +0.2 4,651,518 + BWI76_RS22310 0.89 +0.1 4,651,620 - -0.7 4,651,646 + -0.8 4,651,656 + BWI76_RS22315 0.10 -0.9 4,651,664 - BWI76_RS22315 0.12 +0.8 4,651,664 - BWI76_RS22315 0.12 -0.9 4,652,005 - BWI76_RS22315 0.75 -0.7 4,652,078 + BWI76_RS22315 0.88 +0.3 4,652,102 - +1.6 4,652,190 + +1.0 4,652,212 + +0.1 4,652,234 + +0.3 4,652,257 + -0.3 4,652,257 + +0.8 4,652,265 - -0.2 4,652,281 - +0.3 4,652,309 + +0.1 4,652,392 - BWI76_RS22320 0.14 -0.2 4,652,465 + BWI76_RS22320 0.18 -0.6 4,652,472 + BWI76_RS22320 0.19 -0.6 4,652,473 - BWI76_RS22320 0.19 -0.3 4,652,542 + BWI76_RS22320 0.23 -0.0 4,652,550 - BWI76_RS22320 0.23 -0.9 4,652,550 - BWI76_RS22320 0.23 -0.0 4,652,597 - BWI76_RS22320 0.26 +0.7
Or see this region's nucleotide sequence