Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22310

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22300 and BWI76_RS22305 overlap by 8 nucleotidesBWI76_RS22305 and BWI76_RS22310 overlap by 4 nucleotidesBWI76_RS22310 and BWI76_RS22315 overlap by 1 nucleotidesBWI76_RS22315 and BWI76_RS22320 are separated by 9 nucleotides BWI76_RS22300: BWI76_RS22300 - hydrogenase 3 maturation endopeptidase HyCI, at 4,649,947 to 4,650,432 _RS22300 BWI76_RS22305: BWI76_RS22305 - formate hydrogenlyase maturation protein HycH, at 4,650,425 to 4,650,835 _RS22305 BWI76_RS22310: BWI76_RS22310 - hydrogenase 3 small subunit, at 4,650,832 to 4,651,599 _RS22310 BWI76_RS22315: BWI76_RS22315 - formate hydrogenlyase complex iron-sulfur subunit, at 4,651,599 to 4,652,141 _RS22315 BWI76_RS22320: BWI76_RS22320 - hydrogenase 3 large subunit, at 4,652,151 to 4,653,860 _RS22320 Position (kb) 4650 4651 4652Strain fitness (log2 ratio) -1 0 1at 4649.995 kb on + strandat 4650.366 kb on - strand, within BWI76_RS22300at 4650.494 kb on - strand, within BWI76_RS22305at 4650.990 kb on + strand, within BWI76_RS22310at 4651.316 kb on + strand, within BWI76_RS22310at 4651.334 kb on - strand, within BWI76_RS22310at 4651.414 kb on - strand, within BWI76_RS22310at 4651.414 kb on - strand, within BWI76_RS22310at 4651.518 kb on + strand, within BWI76_RS22310at 4651.620 kb on - strandat 4651.646 kb on + strandat 4651.656 kb on + strand, within BWI76_RS22315at 4651.664 kb on - strand, within BWI76_RS22315at 4651.664 kb on - strand, within BWI76_RS22315at 4652.005 kb on - strand, within BWI76_RS22315at 4652.078 kb on + strand, within BWI76_RS22315at 4652.102 kb on - strandat 4652.190 kb on + strandat 4652.212 kb on + strandat 4652.234 kb on + strandat 4652.257 kb on + strandat 4652.257 kb on + strandat 4652.265 kb on - strandat 4652.281 kb on - strandat 4652.309 kb on + strandat 4652.392 kb on - strand, within BWI76_RS22320at 4652.465 kb on + strand, within BWI76_RS22320at 4652.472 kb on + strand, within BWI76_RS22320at 4652.473 kb on - strand, within BWI76_RS22320at 4652.542 kb on + strand, within BWI76_RS22320at 4652.550 kb on - strand, within BWI76_RS22320at 4652.550 kb on - strand, within BWI76_RS22320at 4652.597 kb on - strand, within BWI76_RS22320

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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4,649,995 + +0.5
4,650,366 - BWI76_RS22300 0.86 -0.1
4,650,494 - BWI76_RS22305 0.17 -0.2
4,650,990 + BWI76_RS22310 0.21 +0.2
4,651,316 + BWI76_RS22310 0.63 -0.2
4,651,334 - BWI76_RS22310 0.65 -1.2
4,651,414 - BWI76_RS22310 0.76 +0.2
4,651,414 - BWI76_RS22310 0.76 +0.2
4,651,518 + BWI76_RS22310 0.89 +0.1
4,651,620 - -0.7
4,651,646 + -0.8
4,651,656 + BWI76_RS22315 0.10 -0.9
4,651,664 - BWI76_RS22315 0.12 +0.8
4,651,664 - BWI76_RS22315 0.12 -0.9
4,652,005 - BWI76_RS22315 0.75 -0.7
4,652,078 + BWI76_RS22315 0.88 +0.3
4,652,102 - +1.6
4,652,190 + +1.0
4,652,212 + +0.1
4,652,234 + +0.3
4,652,257 + -0.3
4,652,257 + +0.8
4,652,265 - -0.2
4,652,281 - +0.3
4,652,309 + +0.1
4,652,392 - BWI76_RS22320 0.14 -0.2
4,652,465 + BWI76_RS22320 0.18 -0.6
4,652,472 + BWI76_RS22320 0.19 -0.6
4,652,473 - BWI76_RS22320 0.19 -0.3
4,652,542 + BWI76_RS22320 0.23 -0.0
4,652,550 - BWI76_RS22320 0.23 -0.9
4,652,550 - BWI76_RS22320 0.23 -0.0
4,652,597 - BWI76_RS22320 0.26 +0.7

Or see this region's nucleotide sequence