Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20145

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20140 and BWI76_RS20145 overlap by 4 nucleotidesBWI76_RS20145 and BWI76_RS20150 overlap by 4 nucleotidesBWI76_RS20150 and BWI76_RS20155 are separated by 10 nucleotides BWI76_RS20140: BWI76_RS20140 - NADH-quinone oxidoreductase subunit L, at 4,220,138 to 4,221,979 _RS20140 BWI76_RS20145: BWI76_RS20145 - NADH-quinone oxidoreductase subunit K, at 4,221,976 to 4,222,278 _RS20145 BWI76_RS20150: BWI76_RS20150 - NADH:ubiquinone oxidoreductase subunit J, at 4,222,275 to 4,222,829 _RS20150 BWI76_RS20155: BWI76_RS20155 - NADH-quinone oxidoreductase subunit I, at 4,222,840 to 4,223,382 _RS20155 Position (kb) 4221 4222 4223Strain fitness (log2 ratio) -2 -1 0 1at 4221.166 kb on + strand, within BWI76_RS20140at 4221.174 kb on - strand, within BWI76_RS20140at 4221.317 kb on + strand, within BWI76_RS20140at 4221.504 kb on + strand, within BWI76_RS20140at 4221.544 kb on + strand, within BWI76_RS20140at 4222.044 kb on + strand, within BWI76_RS20145at 4222.323 kb on + strandat 4222.604 kb on + strand, within BWI76_RS20150at 4222.645 kb on - strand, within BWI76_RS20150at 4222.756 kb on + strand, within BWI76_RS20150at 4222.891 kb on + strandat 4222.899 kb on - strand, within BWI76_RS20155at 4223.157 kb on + strand, within BWI76_RS20155at 4223.190 kb on + strand, within BWI76_RS20155

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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4,221,166 + BWI76_RS20140 0.56 -1.7
4,221,174 - BWI76_RS20140 0.56 -2.2
4,221,317 + BWI76_RS20140 0.64 -2.6
4,221,504 + BWI76_RS20140 0.74 -1.4
4,221,544 + BWI76_RS20140 0.76 -2.2
4,222,044 + BWI76_RS20145 0.22 -2.2
4,222,323 + -1.0
4,222,604 + BWI76_RS20150 0.59 -1.4
4,222,645 - BWI76_RS20150 0.67 -0.4
4,222,756 + BWI76_RS20150 0.87 -0.6
4,222,891 + +0.4
4,222,899 - BWI76_RS20155 0.11 -2.0
4,223,157 + BWI76_RS20155 0.58 -0.5
4,223,190 + BWI76_RS20155 0.64 -2.2

Or see this region's nucleotide sequence