Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19150

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19140 and BWI76_RS19145 are separated by 280 nucleotidesBWI76_RS19145 and BWI76_RS19150 overlap by 4 nucleotidesBWI76_RS19150 and BWI76_RS19155 are separated by 12 nucleotides BWI76_RS19140: BWI76_RS19140 - citrate (Si)-synthase, at 3,979,437 to 3,980,723 _RS19140 BWI76_RS19145: BWI76_RS19145 - formate dehydrogenase cytochrome b556 subunit, at 3,981,004 to 3,981,633 _RS19145 BWI76_RS19150: BWI76_RS19150 - formate dehydrogenase subunit beta, at 3,981,630 to 3,982,532 _RS19150 BWI76_RS19155: BWI76_RS19155 - formate dehydrogenase-N subunit alpha, at 3,982,545 to 3,985,595 _RS19155 Position (kb) 3981 3982 3983Strain fitness (log2 ratio) -1 0 1at 3980.662 kb on + strandat 3981.070 kb on - strand, within BWI76_RS19145at 3982.721 kb on + strandat 3982.783 kb on - strandat 3982.820 kb on - strandat 3982.962 kb on + strand, within BWI76_RS19155at 3983.019 kb on - strand, within BWI76_RS19155at 3983.094 kb on + strand, within BWI76_RS19155at 3983.174 kb on - strand, within BWI76_RS19155at 3983.307 kb on + strand, within BWI76_RS19155at 3983.410 kb on + strand, within BWI76_RS19155at 3983.489 kb on + strand, within BWI76_RS19155at 3983.497 kb on - strand, within BWI76_RS19155

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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3,980,662 + -0.1
3,981,070 - BWI76_RS19145 0.10 -0.6
3,982,721 + +0.5
3,982,783 - -0.7
3,982,820 - +0.0
3,982,962 + BWI76_RS19155 0.14 +0.4
3,983,019 - BWI76_RS19155 0.16 +0.1
3,983,094 + BWI76_RS19155 0.18 +0.3
3,983,174 - BWI76_RS19155 0.21 -0.4
3,983,307 + BWI76_RS19155 0.25 -0.6
3,983,410 + BWI76_RS19155 0.28 -0.7
3,983,489 + BWI76_RS19155 0.31 -0.4
3,983,497 - BWI76_RS19155 0.31 -0.8

Or see this region's nucleotide sequence