Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14970

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14965 and BWI76_RS14970 overlap by 4 nucleotidesBWI76_RS14970 and BWI76_RS14975 are separated by 12 nucleotidesBWI76_RS14975 and BWI76_RS14980 overlap by 4 nucleotides BWI76_RS14965: BWI76_RS14965 - AraC family transcriptional regulator, at 3,137,120 to 3,138,076 _RS14965 BWI76_RS14970: BWI76_RS14970 - pyrroline-5-carboxylate reductase, at 3,138,073 to 3,138,861 _RS14970 BWI76_RS14975: BWI76_RS14975 - glyoxalase-like domain protein, at 3,138,874 to 3,139,629 _RS14975 BWI76_RS14980: BWI76_RS14980 - hypothetical protein, at 3,139,626 to 3,140,156 _RS14980 Position (kb) 3138 3139Strain fitness (log2 ratio) -1 0 1at 3137.141 kb on - strandat 3137.265 kb on + strand, within BWI76_RS14965at 3137.467 kb on + strand, within BWI76_RS14965at 3137.507 kb on - strand, within BWI76_RS14965at 3137.932 kb on - strand, within BWI76_RS14965at 3137.985 kb on - strandat 3138.616 kb on - strand, within BWI76_RS14970at 3139.204 kb on + strand, within BWI76_RS14975at 3139.430 kb on + strand, within BWI76_RS14975at 3139.477 kb on + strand, within BWI76_RS14975at 3139.643 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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3,137,141 - +1.5
3,137,265 + BWI76_RS14965 0.15 -0.5
3,137,467 + BWI76_RS14965 0.36 -0.2
3,137,507 - BWI76_RS14965 0.40 +0.3
3,137,932 - BWI76_RS14965 0.85 -0.1
3,137,985 - +0.4
3,138,616 - BWI76_RS14970 0.69 -1.0
3,139,204 + BWI76_RS14975 0.44 +0.3
3,139,430 + BWI76_RS14975 0.74 -0.2
3,139,477 + BWI76_RS14975 0.80 -1.5
3,139,643 + -0.8

Or see this region's nucleotide sequence