Experiment: 2'-Deoxycytidine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotides BWI76_RS11250 and BWI76_RS11255 overlap by 17 nucleotides BWI76_RS11255 and BWI76_RS11260 overlap by 22 nucleotides BWI76_RS11260 and BWI76_RS11265 are separated by 19 nucleotides
BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035
_RS11245
BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889
_RS11250
BWI76_RS11255: BWI76_RS11255 - putrescine/spermidine ABC transporter ATP-binding protein, at 2,373,873 to 2,375,009
_RS11255
BWI76_RS11260: BWI76_RS11260 - hypothetical protein, at 2,374,988 to 2,375,247
_RS11260
BWI76_RS11265: BWI76_RS11265 - peptidase T, at 2,375,267 to 2,376,496
_RS11265
Position (kb)
2373
2374
2375
2376 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2372.922 kb on + strand, within BWI76_RS11245 at 2373.411 kb on + strand, within BWI76_RS11250 at 2373.732 kb on - strand, within BWI76_RS11250 at 2373.837 kb on - strand at 2374.067 kb on - strand, within BWI76_RS11255 at 2374.067 kb on - strand, within BWI76_RS11255 at 2374.067 kb on - strand, within BWI76_RS11255 at 2375.091 kb on + strand, within BWI76_RS11260 at 2375.650 kb on + strand, within BWI76_RS11265 at 2375.996 kb on - strand, within BWI76_RS11265
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C) remove 2,372,922 + BWI76_RS11245 0.85 -0.5 2,373,411 + BWI76_RS11250 0.44 +0.7 2,373,732 - BWI76_RS11250 0.82 +0.2 2,373,837 - +0.9 2,374,067 - BWI76_RS11255 0.17 -1.8 2,374,067 - BWI76_RS11255 0.17 +0.0 2,374,067 - BWI76_RS11255 0.17 -1.1 2,375,091 + BWI76_RS11260 0.40 +0.4 2,375,650 + BWI76_RS11265 0.31 +1.2 2,375,996 - BWI76_RS11265 0.59 -3.1
Or see this region's nucleotide sequence