Experiment: 2'-Deoxycytidine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04695 and BWI76_RS04700 are separated by 2 nucleotides BWI76_RS04700 and BWI76_RS04705 are separated by 180 nucleotides BWI76_RS04705 and BWI76_RS04710 are separated by 444 nucleotides BWI76_RS04710 and BWI76_RS04715 overlap by 2 nucleotides
BWI76_RS04695: BWI76_RS04695 - acetolactate synthase 3 large subunit, at 979,896 to 981,620
_RS04695
BWI76_RS04700: BWI76_RS04700 - acetolactate synthase small subunit, at 981,623 to 982,114
_RS04700
BWI76_RS04705: BWI76_RS04705 - DNA-binding transcriptional regulator FruR, at 982,295 to 983,299
_RS04705
BWI76_RS04710: BWI76_RS04710 - hypothetical protein, at 983,744 to 983,926
_RS04710
BWI76_RS04715: BWI76_RS04715 - division/cell wall cluster transcriptional repressor MraZ, at 983,925 to 984,383
_RS04715
Position (kb)
982
983
984 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 981.303 kb on + strand, within BWI76_RS04695 at 981.351 kb on + strand, within BWI76_RS04695 at 981.364 kb on - strand, within BWI76_RS04695 at 981.388 kb on - strand, within BWI76_RS04695 at 981.388 kb on - strand, within BWI76_RS04695 at 981.542 kb on - strand at 981.551 kb on + strand at 981.551 kb on + strand at 981.551 kb on + strand at 981.559 kb on - strand at 981.593 kb on - strand at 981.617 kb on - strand at 981.665 kb on + strand at 981.801 kb on + strand, within BWI76_RS04700 at 981.884 kb on - strand, within BWI76_RS04700 at 982.063 kb on + strand, within BWI76_RS04700 at 982.068 kb on + strand at 982.222 kb on + strand at 982.222 kb on + strand at 982.340 kb on + strand at 982.631 kb on + strand, within BWI76_RS04705 at 982.647 kb on - strand, within BWI76_RS04705 at 982.684 kb on - strand, within BWI76_RS04705 at 982.730 kb on + strand, within BWI76_RS04705 at 982.730 kb on + strand, within BWI76_RS04705 at 982.757 kb on + strand, within BWI76_RS04705 at 982.789 kb on - strand, within BWI76_RS04705 at 983.020 kb on + strand, within BWI76_RS04705 at 983.028 kb on - strand, within BWI76_RS04705 at 983.066 kb on - strand, within BWI76_RS04705 at 983.186 kb on - strand, within BWI76_RS04705 at 983.251 kb on - strand at 983.265 kb on + strand at 983.282 kb on + strand at 983.297 kb on - strand at 983.318 kb on - strand at 983.324 kb on + strand at 983.344 kb on - strand at 983.346 kb on - strand at 983.393 kb on - strand at 983.414 kb on - strand at 983.474 kb on + strand at 983.482 kb on - strand at 983.554 kb on + strand at 983.565 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.573 kb on - strand at 983.574 kb on + strand at 983.575 kb on + strand at 983.577 kb on + strand at 983.577 kb on + strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.582 kb on - strand at 983.619 kb on + strand at 983.626 kb on - strand at 983.627 kb on - strand at 983.636 kb on - strand at 983.637 kb on + strand at 983.638 kb on + strand at 983.670 kb on - strand at 983.710 kb on + strand at 983.718 kb on - strand at 983.824 kb on + strand, within BWI76_RS04710 at 983.826 kb on + strand, within BWI76_RS04710 at 983.834 kb on - strand, within BWI76_RS04710 at 983.849 kb on - strand, within BWI76_RS04710 at 983.922 kb on + strand at 984.062 kb on + strand, within BWI76_RS04715
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C) remove 981,303 + BWI76_RS04695 0.82 +0.8 981,351 + BWI76_RS04695 0.84 -0.2 981,364 - BWI76_RS04695 0.85 +1.6 981,388 - BWI76_RS04695 0.86 +0.6 981,388 - BWI76_RS04695 0.86 +0.4 981,542 - -0.3 981,551 + -0.4 981,551 + -0.1 981,551 + -0.2 981,559 - +0.8 981,593 - -0.5 981,617 - +1.0 981,665 + -0.4 981,801 + BWI76_RS04700 0.36 -0.4 981,884 - BWI76_RS04700 0.53 +0.3 982,063 + BWI76_RS04700 0.89 -0.5 982,068 + -0.2 982,222 + +0.2 982,222 + -0.0 982,340 + -1.0 982,631 + BWI76_RS04705 0.33 -3.3 982,647 - BWI76_RS04705 0.35 -4.0 982,684 - BWI76_RS04705 0.39 +0.0 982,730 + BWI76_RS04705 0.43 -3.0 982,730 + BWI76_RS04705 0.43 -0.6 982,757 + BWI76_RS04705 0.46 -1.6 982,789 - BWI76_RS04705 0.49 -0.8 983,020 + BWI76_RS04705 0.72 -1.7 983,028 - BWI76_RS04705 0.73 -1.5 983,066 - BWI76_RS04705 0.77 -2.0 983,186 - BWI76_RS04705 0.89 -2.0 983,251 - -1.0 983,265 + +1.3 983,282 + +0.8 983,297 - +0.0 983,318 - -1.0 983,324 + +0.2 983,344 - -0.4 983,346 - +1.1 983,393 - +0.4 983,414 - +0.9 983,474 + -1.1 983,482 - +0.1 983,554 + +0.5 983,565 + -0.4 983,572 + -0.7 983,572 + -1.7 983,572 + +0.9 983,572 + -0.3 983,572 + -0.1 983,572 + -0.1 983,573 - -0.0 983,574 + +0.1 983,575 + -0.1 983,577 + +0.2 983,577 + +0.3 983,580 - -0.9 983,580 - -0.2 983,580 - -1.1 983,580 - +0.3 983,580 - -0.1 983,580 - -0.1 983,580 - -0.4 983,580 - +0.4 983,580 - -0.8 983,580 - +0.6 983,580 - +0.1 983,580 - +0.5 983,580 - +0.5 983,582 - -0.0 983,619 + -0.1 983,626 - -0.3 983,627 - +0.2 983,636 - +1.0 983,637 + +0.0 983,638 + +0.9 983,670 - -1.3 983,710 + +0.7 983,718 - +0.9 983,824 + BWI76_RS04710 0.44 -2.1 983,826 + BWI76_RS04710 0.45 +2.6 983,834 - BWI76_RS04710 0.49 -0.1 983,849 - BWI76_RS04710 0.57 -0.1 983,922 + -1.2 984,062 + BWI76_RS04715 0.30 -1.2
Or see this region's nucleotide sequence