Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04135

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04130 and BWI76_RS04135 are separated by 77 nucleotidesBWI76_RS04135 and BWI76_RS04140 are separated by 52 nucleotides BWI76_RS04130: BWI76_RS04130 - 2-deoxyribose-5-phosphate aldolase, at 849,568 to 850,347 _RS04130 BWI76_RS04135: BWI76_RS04135 - thymidine phosphorylase, at 850,425 to 851,747 _RS04135 BWI76_RS04140: BWI76_RS04140 - phosphopentomutase, at 851,800 to 853,023 _RS04140 Position (kb) 850 851 852Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 849.483 kb on - strandat 849.607 kb on - strandat 849.727 kb on - strand, within BWI76_RS04130at 849.761 kb on + strand, within BWI76_RS04130at 849.776 kb on + strand, within BWI76_RS04130at 849.828 kb on + strand, within BWI76_RS04130at 849.870 kb on - strand, within BWI76_RS04130at 849.928 kb on - strand, within BWI76_RS04130at 849.933 kb on - strand, within BWI76_RS04130at 850.241 kb on - strand, within BWI76_RS04130at 850.396 kb on + strandat 850.652 kb on - strand, within BWI76_RS04135at 850.836 kb on + strand, within BWI76_RS04135at 851.062 kb on + strand, within BWI76_RS04135at 851.062 kb on + strand, within BWI76_RS04135at 851.077 kb on - strand, within BWI76_RS04135at 851.136 kb on + strandat 851.331 kb on + strand, within BWI76_RS04135at 851.339 kb on - strand, within BWI76_RS04135at 851.339 kb on - strand, within BWI76_RS04135at 851.661 kb on - strandat 851.663 kb on + strandat 851.752 kb on + strandat 851.755 kb on - strandat 851.760 kb on - strandat 851.896 kb on - strandat 851.972 kb on + strand, within BWI76_RS04140at 851.980 kb on - strand, within BWI76_RS04140at 851.990 kb on - strand, within BWI76_RS04140at 852.095 kb on + strand, within BWI76_RS04140at 852.195 kb on + strand, within BWI76_RS04140at 852.378 kb on + strand, within BWI76_RS04140at 852.585 kb on + strand, within BWI76_RS04140

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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849,483 - -3.1
849,607 - -5.2
849,727 - BWI76_RS04130 0.20 -3.9
849,761 + BWI76_RS04130 0.25 -0.5
849,776 + BWI76_RS04130 0.27 -4.7
849,828 + BWI76_RS04130 0.33 -4.8
849,870 - BWI76_RS04130 0.39 -3.6
849,928 - BWI76_RS04130 0.46 -3.2
849,933 - BWI76_RS04130 0.47 -4.7
850,241 - BWI76_RS04130 0.86 -2.4
850,396 + -2.5
850,652 - BWI76_RS04135 0.17 -4.4
850,836 + BWI76_RS04135 0.31 -4.0
851,062 + BWI76_RS04135 0.48 -3.5
851,062 + BWI76_RS04135 0.48 -4.1
851,077 - BWI76_RS04135 0.49 -2.9
851,136 + +0.1
851,331 + BWI76_RS04135 0.68 -5.5
851,339 - BWI76_RS04135 0.69 -4.9
851,339 - BWI76_RS04135 0.69 -3.4
851,661 - -4.3
851,663 + -4.8
851,752 + -2.6
851,755 - -1.5
851,760 - -2.9
851,896 - -4.1
851,972 + BWI76_RS04140 0.14 -1.4
851,980 - BWI76_RS04140 0.15 -2.5
851,990 - BWI76_RS04140 0.16 -3.1
852,095 + BWI76_RS04140 0.24 -4.5
852,195 + BWI76_RS04140 0.32 -1.9
852,378 + BWI76_RS04140 0.47 +0.1
852,585 + BWI76_RS04140 0.64 -5.4

Or see this region's nucleotide sequence