Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02530

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02525 and BWI76_RS02530 overlap by 8 nucleotidesBWI76_RS02530 and BWI76_RS02535 are separated by 92 nucleotidesBWI76_RS02535 and BWI76_RS02540 are separated by 75 nucleotides BWI76_RS02525: BWI76_RS02525 - DNA mismatch repair protein MutL, at 517,026 to 518,921 _RS02525 BWI76_RS02530: BWI76_RS02530 - tRNA dimethylallyltransferase, at 518,914 to 519,864 _RS02530 BWI76_RS02535: BWI76_RS02535 - RNA-binding protein Hfq, at 519,957 to 520,265 _RS02535 BWI76_RS02540: BWI76_RS02540 - GTPase HflX, at 520,341 to 521,621 _RS02540 Position (kb) 518 519 520Strain fitness (log2 ratio) -3 -2 -1 0 1at 517.926 kb on - strand, within BWI76_RS02525at 518.074 kb on - strand, within BWI76_RS02525at 518.152 kb on + strand, within BWI76_RS02525at 518.287 kb on + strand, within BWI76_RS02525at 518.325 kb on + strand, within BWI76_RS02525at 518.468 kb on - strand, within BWI76_RS02525at 518.502 kb on + strand, within BWI76_RS02525at 518.512 kb on + strand, within BWI76_RS02525at 518.621 kb on - strand, within BWI76_RS02525at 518.651 kb on + strand, within BWI76_RS02525at 518.652 kb on - strand, within BWI76_RS02525at 518.659 kb on - strand, within BWI76_RS02525at 518.758 kb on + strandat 518.816 kb on + strandat 518.846 kb on + strandat 518.854 kb on - strandat 518.854 kb on - strandat 518.940 kb on + strandat 519.180 kb on + strand, within BWI76_RS02530at 519.287 kb on - strand, within BWI76_RS02530at 519.287 kb on - strand, within BWI76_RS02530at 519.305 kb on - strand, within BWI76_RS02530at 519.530 kb on + strand, within BWI76_RS02530at 519.776 kb on + strandat 519.853 kb on + strandat 519.882 kb on + strandat 519.922 kb on + strandat 520.239 kb on + strandat 520.333 kb on + strandat 520.365 kb on - strandat 520.427 kb on - strandat 520.528 kb on - strand, within BWI76_RS02540at 520.794 kb on - strand, within BWI76_RS02540at 520.799 kb on - strand, within BWI76_RS02540at 520.825 kb on - strand, within BWI76_RS02540at 520.849 kb on + strand, within BWI76_RS02540

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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517,926 - BWI76_RS02525 0.47 +0.7
518,074 - BWI76_RS02525 0.55 -0.7
518,152 + BWI76_RS02525 0.59 +0.0
518,287 + BWI76_RS02525 0.67 -1.6
518,325 + BWI76_RS02525 0.69 +0.1
518,468 - BWI76_RS02525 0.76 -1.2
518,502 + BWI76_RS02525 0.78 +0.2
518,512 + BWI76_RS02525 0.78 -0.6
518,621 - BWI76_RS02525 0.84 +0.4
518,651 + BWI76_RS02525 0.86 +0.3
518,652 - BWI76_RS02525 0.86 +0.6
518,659 - BWI76_RS02525 0.86 +0.8
518,758 + -0.4
518,816 + -1.6
518,846 + -0.1
518,854 - -0.8
518,854 - -0.4
518,940 + -1.2
519,180 + BWI76_RS02530 0.28 -0.6
519,287 - BWI76_RS02530 0.39 -1.2
519,287 - BWI76_RS02530 0.39 -2.3
519,305 - BWI76_RS02530 0.41 +0.2
519,530 + BWI76_RS02530 0.65 -3.3
519,776 + -0.2
519,853 + -0.1
519,882 + -0.2
519,922 + +0.1
520,239 + -2.2
520,333 + +0.2
520,365 - -1.2
520,427 - -0.8
520,528 - BWI76_RS02540 0.15 -0.4
520,794 - BWI76_RS02540 0.35 +0.4
520,799 - BWI76_RS02540 0.36 -0.8
520,825 - BWI76_RS02540 0.38 -0.3
520,849 + BWI76_RS02540 0.40 -1.6

Or see this region's nucleotide sequence