Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00455

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00450 and BWI76_RS00455 are separated by 343 nucleotidesBWI76_RS00455 and BWI76_RS00460 overlap by 4 nucleotidesBWI76_RS00460 and BWI76_RS00465 are separated by 12 nucleotides BWI76_RS00450: BWI76_RS00450 - formate dehydrogenase accessory protein FdhE, at 91,941 to 92,870 _RS00450 BWI76_RS00455: BWI76_RS00455 - formate dehydrogenase cytochrome b556 subunit, at 93,214 to 93,849 _RS00455 BWI76_RS00460: BWI76_RS00460 - formate dehydrogenase subunit beta, at 93,846 to 94,748 _RS00460 BWI76_RS00465: BWI76_RS00465 - formate dehydrogenase-N subunit alpha, at 94,761 to 97,175 _RS00465 Position (kb) 93 94Strain fitness (log2 ratio) -2 -1 0 1at 92.222 kb on + strand, within BWI76_RS00450at 92.226 kb on + strand, within BWI76_RS00450at 92.251 kb on - strand, within BWI76_RS00450at 92.555 kb on + strand, within BWI76_RS00450at 92.601 kb on + strand, within BWI76_RS00450at 92.610 kb on - strand, within BWI76_RS00450at 92.682 kb on - strand, within BWI76_RS00450at 92.851 kb on - strandat 93.001 kb on - strandat 93.015 kb on + strandat 93.037 kb on + strandat 93.052 kb on - strandat 93.067 kb on + strandat 93.079 kb on + strandat 93.123 kb on + strandat 93.135 kb on - strandat 94.744 kb on + strandat 94.789 kb on + strandat 94.789 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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92,222 + BWI76_RS00450 0.30 -0.3
92,226 + BWI76_RS00450 0.31 -0.5
92,251 - BWI76_RS00450 0.33 -0.6
92,555 + BWI76_RS00450 0.66 -0.9
92,601 + BWI76_RS00450 0.71 -0.1
92,610 - BWI76_RS00450 0.72 +0.9
92,682 - BWI76_RS00450 0.80 -0.7
92,851 - -1.1
93,001 - -0.7
93,015 + +0.1
93,037 + -0.6
93,052 - -0.4
93,067 + -0.2
93,079 + +0.5
93,123 + -1.1
93,135 - +1.7
94,744 + +1.2
94,789 + -1.9
94,789 + +0.1

Or see this region's nucleotide sequence