Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00450

Experiment: 2'-Deoxycytidine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00435 and BWI76_RS00440 are separated by 205 nucleotidesBWI76_RS00440 and BWI76_RS00445 overlap by 4 nucleotidesBWI76_RS00445 and BWI76_RS00450 are separated by 37 nucleotidesBWI76_RS00450 and BWI76_RS00455 are separated by 343 nucleotidesBWI76_RS00455 and BWI76_RS00460 overlap by 4 nucleotides BWI76_RS00435: BWI76_RS00435 - lipase, at 90,170 to 91,087 _RS00435 BWI76_RS00440: BWI76_RS00440 - CopG family transcriptional regulator, at 91,293 to 91,532 _RS00440 BWI76_RS00445: BWI76_RS00445 - PIN domain nuclease, at 91,529 to 91,903 _RS00445 BWI76_RS00450: BWI76_RS00450 - formate dehydrogenase accessory protein FdhE, at 91,941 to 92,870 _RS00450 BWI76_RS00455: BWI76_RS00455 - formate dehydrogenase cytochrome b556 subunit, at 93,214 to 93,849 _RS00455 BWI76_RS00460: BWI76_RS00460 - formate dehydrogenase subunit beta, at 93,846 to 94,748 _RS00460 Position (kb) 91 92 93Strain fitness (log2 ratio) -1 0 1at 91.063 kb on + strandat 91.279 kb on + strandat 91.308 kb on + strandat 91.364 kb on + strand, within BWI76_RS00440at 91.645 kb on - strand, within BWI76_RS00445at 91.693 kb on + strand, within BWI76_RS00445at 91.746 kb on + strand, within BWI76_RS00445at 91.783 kb on + strand, within BWI76_RS00445at 91.800 kb on + strand, within BWI76_RS00445at 91.807 kb on + strand, within BWI76_RS00445at 91.807 kb on + strand, within BWI76_RS00445at 91.807 kb on + strand, within BWI76_RS00445at 91.815 kb on - strand, within BWI76_RS00445at 91.830 kb on + strand, within BWI76_RS00445at 91.830 kb on + strand, within BWI76_RS00445at 91.830 kb on + strand, within BWI76_RS00445at 91.934 kb on - strandat 91.934 kb on - strandat 91.970 kb on + strandat 91.978 kb on - strandat 92.014 kb on + strandat 92.222 kb on + strand, within BWI76_RS00450at 92.226 kb on + strand, within BWI76_RS00450at 92.251 kb on - strand, within BWI76_RS00450at 92.555 kb on + strand, within BWI76_RS00450at 92.601 kb on + strand, within BWI76_RS00450at 92.610 kb on - strand, within BWI76_RS00450at 92.682 kb on - strand, within BWI76_RS00450at 92.851 kb on - strandat 93.001 kb on - strandat 93.015 kb on + strandat 93.037 kb on + strandat 93.052 kb on - strandat 93.067 kb on + strandat 93.079 kb on + strandat 93.123 kb on + strandat 93.135 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxycytidine 10 mM (C)
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91,063 + +1.3
91,279 + +1.4
91,308 + -0.0
91,364 + BWI76_RS00440 0.30 -1.1
91,645 - BWI76_RS00445 0.31 +1.1
91,693 + BWI76_RS00445 0.44 -0.6
91,746 + BWI76_RS00445 0.58 +1.0
91,783 + BWI76_RS00445 0.68 +0.2
91,800 + BWI76_RS00445 0.72 +1.3
91,807 + BWI76_RS00445 0.74 +0.4
91,807 + BWI76_RS00445 0.74 -0.1
91,807 + BWI76_RS00445 0.74 -0.1
91,815 - BWI76_RS00445 0.76 -1.2
91,830 + BWI76_RS00445 0.80 -0.5
91,830 + BWI76_RS00445 0.80 +1.3
91,830 + BWI76_RS00445 0.80 +1.5
91,934 - +0.3
91,934 - +0.3
91,970 + +1.6
91,978 - +0.2
92,014 + -0.2
92,222 + BWI76_RS00450 0.30 -0.3
92,226 + BWI76_RS00450 0.31 -0.5
92,251 - BWI76_RS00450 0.33 -0.6
92,555 + BWI76_RS00450 0.66 -0.9
92,601 + BWI76_RS00450 0.71 -0.1
92,610 - BWI76_RS00450 0.72 +0.9
92,682 - BWI76_RS00450 0.80 -0.7
92,851 - -1.1
93,001 - -0.7
93,015 + +0.1
93,037 + -0.6
93,052 - -0.4
93,067 + -0.2
93,079 + +0.5
93,123 + -1.1
93,135 - +1.7

Or see this region's nucleotide sequence