Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4385 and Psyr_4386 are separated by 330 nucleotides Psyr_4386 and Psyr_4387 are separated by 95 nucleotides
Psyr_4385: Psyr_4385 - Alpha-2-macroglobulin, N-terminal:Alpha-2-macroglobulin, N-terminal, at 5,242,625 to 5,247,583
_4385
Psyr_4386: Psyr_4386 - conserved hypothetical protein, at 5,247,914 to 5,248,207
_4386
Psyr_4387: Psyr_4387 - Glycosyl transferase, family 51:Penicillin-binding protein, transpeptidase:Penicillin-binding, C-terminal, at 5,248,303 to 5,250,651
_4387
Position (kb)
5247
5248
5249 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5247.149 kb on + strand at 5247.149 kb on + strand at 5247.150 kb on - strand at 5247.411 kb on - strand at 5247.411 kb on - strand at 5247.458 kb on + strand at 5247.459 kb on - strand at 5247.459 kb on - strand at 5247.459 kb on - strand at 5247.612 kb on + strand at 5247.704 kb on - strand at 5247.759 kb on + strand at 5247.767 kb on + strand at 5247.901 kb on - strand at 5248.008 kb on + strand, within Psyr_4386 at 5248.008 kb on + strand, within Psyr_4386 at 5248.008 kb on + strand, within Psyr_4386 at 5248.008 kb on + strand, within Psyr_4386 at 5248.009 kb on - strand, within Psyr_4386 at 5248.009 kb on - strand, within Psyr_4386 at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.200 kb on - strand at 5248.200 kb on - strand at 5248.200 kb on - strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.288 kb on + strand at 5248.288 kb on + strand at 5248.493 kb on + strand at 5248.494 kb on - strand at 5248.494 kb on - strand at 5248.532 kb on - strand at 5248.534 kb on + strand at 5248.534 kb on + strand at 5248.535 kb on - strand at 5248.535 kb on - strand at 5248.535 kb on - strand at 5248.555 kb on + strand, within Psyr_4387 at 5248.556 kb on - strand, within Psyr_4387 at 5248.556 kb on - strand, within Psyr_4387 at 5248.573 kb on + strand, within Psyr_4387 at 5248.574 kb on - strand, within Psyr_4387 at 5248.643 kb on + strand, within Psyr_4387 at 5248.644 kb on - strand, within Psyr_4387 at 5248.644 kb on - strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.786 kb on + strand, within Psyr_4387 at 5248.787 kb on - strand, within Psyr_4387 at 5248.918 kb on - strand, within Psyr_4387
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 5,247,149 + +1.0 5,247,149 + -0.1 5,247,150 - +0.4 5,247,411 - -0.8 5,247,411 - -0.5 5,247,458 + +0.3 5,247,459 - -1.0 5,247,459 - -1.1 5,247,459 - -1.1 5,247,612 + +0.6 5,247,704 - -0.3 5,247,759 + -0.4 5,247,767 + +2.4 5,247,901 - -1.0 5,248,008 + Psyr_4386 0.32 -0.6 5,248,008 + Psyr_4386 0.32 -0.4 5,248,008 + Psyr_4386 0.32 -2.8 5,248,008 + Psyr_4386 0.32 -1.1 5,248,009 - Psyr_4386 0.32 -1.0 5,248,009 - Psyr_4386 0.32 -0.4 5,248,199 + -0.1 5,248,199 + +0.7 5,248,199 + -1.8 5,248,199 + -0.8 5,248,199 + +0.6 5,248,199 + +1.0 5,248,200 - -0.1 5,248,200 - +0.1 5,248,200 - -0.3 5,248,207 + -0.2 5,248,207 + +0.4 5,248,207 + -0.4 5,248,207 + +1.1 5,248,207 + +0.8 5,248,207 + +2.0 5,248,207 + -0.1 5,248,207 + +0.8 5,248,207 + -2.8 5,248,207 + -0.2 5,248,207 + +0.4 5,248,207 + -1.3 5,248,207 + -0.3 5,248,207 + -0.6 5,248,207 + +0.0 5,248,207 + +0.4 5,248,207 + +0.1 5,248,207 + -0.2 5,248,207 + -0.4 5,248,207 + +0.6 5,248,207 + -0.4 5,248,208 - +0.5 5,248,208 - +0.4 5,248,208 - +0.3 5,248,208 - -0.5 5,248,208 - -0.1 5,248,208 - -1.7 5,248,208 - +0.2 5,248,208 - -1.3 5,248,208 - -0.5 5,248,208 - -0.2 5,248,208 - +1.3 5,248,208 - +0.6 5,248,208 - -0.3 5,248,208 - -0.0 5,248,208 - -0.6 5,248,208 - +0.8 5,248,208 - +0.3 5,248,208 - -1.6 5,248,208 - -1.6 5,248,208 - -0.7 5,248,208 - -0.7 5,248,208 - -0.6 5,248,208 - +0.3 5,248,208 - -0.7 5,248,208 - +0.4 5,248,208 - +1.0 5,248,208 - -0.1 5,248,208 - +0.1 5,248,208 - -0.5 5,248,208 - -0.8 5,248,211 + -0.8 5,248,211 + +0.4 5,248,211 + +0.4 5,248,211 + -0.2 5,248,211 + -0.1 5,248,211 + -0.3 5,248,211 + +0.0 5,248,211 + +0.3 5,248,211 + +0.7 5,248,211 + +0.5 5,248,211 + -1.0 5,248,211 + +0.4 5,248,211 + -1.6 5,248,211 + +1.1 5,248,211 + +0.8 5,248,211 + +0.6 5,248,211 + +0.1 5,248,211 + +0.7 5,248,211 + +0.2 5,248,211 + +0.3 5,248,212 - -0.4 5,248,212 - +0.2 5,248,212 - -0.1 5,248,212 - -0.6 5,248,212 - -0.7 5,248,212 - -0.2 5,248,212 - -0.4 5,248,212 - +0.0 5,248,212 - -0.9 5,248,212 - +0.4 5,248,212 - +0.3 5,248,212 - +0.2 5,248,212 - -0.4 5,248,212 - -0.6 5,248,212 - -0.5 5,248,212 - -0.1 5,248,212 - -1.2 5,248,212 - -3.7 5,248,212 - -0.9 5,248,212 - +0.7 5,248,212 - -0.5 5,248,212 - -0.2 5,248,212 - +1.6 5,248,288 + -0.6 5,248,288 + -0.1 5,248,493 + +0.5 5,248,494 - -0.1 5,248,494 - -0.2 5,248,532 - -0.1 5,248,534 + -1.2 5,248,534 + -0.1 5,248,535 - -0.0 5,248,535 - +0.6 5,248,535 - -0.2 5,248,555 + Psyr_4387 0.11 -0.3 5,248,556 - Psyr_4387 0.11 -0.3 5,248,556 - Psyr_4387 0.11 -0.0 5,248,573 + Psyr_4387 0.11 -0.9 5,248,574 - Psyr_4387 0.12 +0.6 5,248,643 + Psyr_4387 0.14 +0.6 5,248,644 - Psyr_4387 0.15 -2.3 5,248,644 - Psyr_4387 0.15 +0.9 5,248,704 + Psyr_4387 0.17 +0.2 5,248,704 + Psyr_4387 0.17 +0.2 5,248,704 + Psyr_4387 0.17 -0.2 5,248,704 + Psyr_4387 0.17 -1.7 5,248,704 + Psyr_4387 0.17 -0.7 5,248,704 + Psyr_4387 0.17 +0.2 5,248,705 - Psyr_4387 0.17 +0.3 5,248,705 - Psyr_4387 0.17 +0.5 5,248,705 - Psyr_4387 0.17 -0.6 5,248,705 - Psyr_4387 0.17 -2.3 5,248,705 - Psyr_4387 0.17 -0.3 5,248,705 - Psyr_4387 0.17 -0.8 5,248,705 - Psyr_4387 0.17 -1.0 5,248,705 - Psyr_4387 0.17 +0.6 5,248,705 - Psyr_4387 0.17 +1.1 5,248,705 - Psyr_4387 0.17 +0.1 5,248,705 - Psyr_4387 0.17 -0.1 5,248,705 - Psyr_4387 0.17 +0.1 5,248,786 + Psyr_4387 0.21 +1.0 5,248,787 - Psyr_4387 0.21 +0.5 5,248,918 - Psyr_4387 0.26 +0.0
Or see this region's nucleotide sequence