Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3949 and Psyr_3950 are separated by 60 nucleotides Psyr_3950 and Psyr_3951 overlap by 8 nucleotides Psyr_3951 and Psyr_3952 overlap by 4 nucleotides Psyr_3952 and Psyr_3953 are separated by 114 nucleotides
Psyr_3949: Psyr_3949 - DNA replication and repair protein RecO, at 4,697,047 to 4,697,730
_3949
Psyr_3950: Psyr_3950 - Small GTP-binding protein domain:GTP-binding:GTP-binding protein Era, at 4,697,791 to 4,698,693
_3950
Psyr_3951: Psyr_3951 - RNAse III, at 4,698,686 to 4,699,375
_3951
Psyr_3952: Psyr_3952 - conserved hypothetical protein, at 4,699,372 to 4,699,749
_3952
Psyr_3953: Psyr_3953 - signal peptidase I, Serine peptidase, MEROPS family S26A, at 4,699,864 to 4,700,718
_3953
Position (kb)
4698
4699
4700 Strain fitness (log2 ratio)
-2
-1
0
1 at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.702 kb on + strand at 4697.703 kb on - strand at 4697.703 kb on - strand at 4697.703 kb on - strand at 4697.703 kb on - strand at 4697.703 kb on - strand at 4697.703 kb on - strand at 4697.793 kb on - strand at 4697.793 kb on - strand at 4698.792 kb on - strand, within Psyr_3951 at 4699.452 kb on - strand, within Psyr_3952 at 4699.514 kb on - strand, within Psyr_3952 at 4699.554 kb on - strand, within Psyr_3952 at 4699.635 kb on - strand, within Psyr_3952 at 4699.635 kb on - strand, within Psyr_3952 at 4699.644 kb on - strand, within Psyr_3952 at 4699.738 kb on - strand at 4699.738 kb on - strand at 4699.738 kb on - strand at 4699.738 kb on - strand at 4699.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 4,697,702 + +0.0 4,697,702 + -0.2 4,697,702 + +0.8 4,697,702 + -0.8 4,697,702 + -0.2 4,697,702 + +0.3 4,697,702 + +0.2 4,697,702 + -0.3 4,697,702 + -0.8 4,697,702 + +1.7 4,697,702 + -1.2 4,697,703 - -1.5 4,697,703 - +0.1 4,697,703 - -0.1 4,697,703 - -0.1 4,697,703 - +1.0 4,697,703 - -0.7 4,697,793 - -1.5 4,697,793 - +0.5 4,698,792 - Psyr_3951 0.15 +1.3 4,699,452 - Psyr_3952 0.21 -1.0 4,699,514 - Psyr_3952 0.38 -2.4 4,699,554 - Psyr_3952 0.48 +0.3 4,699,635 - Psyr_3952 0.70 -1.1 4,699,635 - Psyr_3952 0.70 -0.4 4,699,644 - Psyr_3952 0.72 -1.7 4,699,738 - +0.3 4,699,738 - -0.7 4,699,738 - +0.3 4,699,738 - +0.8 4,699,738 - -0.7
Or see this region's nucleotide sequence