Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3754 and Psyr_3755 are separated by 313 nucleotides Psyr_3755 and Psyr_3756 are separated by 119 nucleotides Psyr_3756 and Psyr_3757 overlap by 4 nucleotides Psyr_3757 and Psyr_3758 are separated by 234 nucleotides
Psyr_3754: Psyr_3754 - conserved hypothetical protein, at 4,471,534 to 4,471,914
_3754
Psyr_3755: Psyr_3755 - conserved hypothetical protein, at 4,472,228 to 4,472,386
_3755
Psyr_3756: Psyr_3756 - Na+/solute symporter, at 4,472,506 to 4,474,164
_3756
Psyr_3757: Psyr_3757 - Protein of unknown function DUF485, at 4,474,161 to 4,474,472
_3757
Psyr_3758: Psyr_3758 - Substrate-binding region of ABC-type glycine betaine transport system, at 4,474,707 to 4,475,561
_3758
Position (kb) 4472
4473
4474
4475 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4471.559 kb on + strand at 4471.559 kb on + strand at 4471.580 kb on + strand, within Psyr_3754 at 4471.580 kb on + strand, within Psyr_3754 at 4471.580 kb on + strand, within Psyr_3754 at 4471.580 kb on + strand, within Psyr_3754 at 4471.627 kb on + strand, within Psyr_3754 at 4471.670 kb on + strand, within Psyr_3754 at 4471.670 kb on + strand, within Psyr_3754 at 4471.695 kb on - strand, within Psyr_3754 at 4471.751 kb on + strand, within Psyr_3754 at 4471.751 kb on + strand, within Psyr_3754 at 4471.752 kb on - strand, within Psyr_3754 at 4471.828 kb on + strand, within Psyr_3754 at 4471.867 kb on + strand, within Psyr_3754 at 4471.867 kb on + strand, within Psyr_3754 at 4471.867 kb on + strand, within Psyr_3754 at 4471.868 kb on - strand, within Psyr_3754 at 4471.868 kb on - strand, within Psyr_3754 at 4472.196 kb on + strand at 4472.201 kb on - strand at 4472.201 kb on - strand at 4472.207 kb on - strand at 4472.207 kb on - strand at 4472.211 kb on + strand at 4472.212 kb on - strand at 4472.248 kb on + strand, within Psyr_3755 at 4472.272 kb on - strand, within Psyr_3755 at 4472.315 kb on + strand, within Psyr_3755 at 4472.382 kb on + strand at 4472.382 kb on + strand at 4472.382 kb on + strand at 4472.382 kb on + strand at 4472.383 kb on - strand at 4472.411 kb on + strand at 4472.412 kb on - strand at 4472.412 kb on - strand at 4472.412 kb on - strand at 4472.413 kb on + strand at 4472.413 kb on + strand at 4472.413 kb on + strand at 4472.413 kb on + strand at 4472.413 kb on + strand at 4472.413 kb on + strand at 4472.414 kb on - strand at 4472.414 kb on - strand at 4472.414 kb on - strand at 4472.421 kb on - strand at 4472.507 kb on + strand at 4472.508 kb on - strand at 4472.508 kb on - strand at 4472.565 kb on - strand at 4472.625 kb on + strand at 4472.625 kb on + strand at 4472.630 kb on - strand at 4472.630 kb on - strand at 4472.801 kb on + strand, within Psyr_3756 at 4472.801 kb on + strand, within Psyr_3756 at 4472.801 kb on + strand, within Psyr_3756 at 4472.801 kb on + strand, within Psyr_3756 at 4472.802 kb on - strand, within Psyr_3756 at 4472.996 kb on + strand, within Psyr_3756 at 4472.996 kb on + strand, within Psyr_3756 at 4472.996 kb on + strand, within Psyr_3756 at 4472.996 kb on + strand, within Psyr_3756 at 4472.996 kb on + strand, within Psyr_3756 at 4472.996 kb on + strand, within Psyr_3756 at 4472.997 kb on - strand, within Psyr_3756 at 4472.997 kb on - strand, within Psyr_3756 at 4472.997 kb on - strand, within Psyr_3756 at 4472.997 kb on - strand, within Psyr_3756 at 4472.997 kb on - strand, within Psyr_3756 at 4472.997 kb on - strand, within Psyr_3756 at 4473.233 kb on + strand, within Psyr_3756 at 4473.233 kb on + strand, within Psyr_3756 at 4473.233 kb on + strand, within Psyr_3756 at 4473.234 kb on - strand, within Psyr_3756 at 4473.239 kb on + strand, within Psyr_3756 at 4473.240 kb on - strand, within Psyr_3756 at 4473.261 kb on - strand, within Psyr_3756 at 4473.261 kb on - strand, within Psyr_3756 at 4473.575 kb on + strand, within Psyr_3756 at 4473.575 kb on + strand, within Psyr_3756 at 4473.575 kb on + strand, within Psyr_3756 at 4473.575 kb on + strand, within Psyr_3756 at 4473.575 kb on + strand, within Psyr_3756 at 4473.576 kb on - strand, within Psyr_3756 at 4473.576 kb on - strand, within Psyr_3756 at 4473.576 kb on - strand, within Psyr_3756 at 4473.576 kb on - strand, within Psyr_3756 at 4473.576 kb on - strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.767 kb on + strand, within Psyr_3756 at 4473.768 kb on - strand, within Psyr_3756 at 4473.768 kb on - strand, within Psyr_3756 at 4473.768 kb on - strand at 4473.768 kb on - strand, within Psyr_3756 at 4473.824 kb on - strand, within Psyr_3756 at 4473.842 kb on + strand, within Psyr_3756 at 4473.843 kb on - strand, within Psyr_3756 at 4473.843 kb on - strand, within Psyr_3756 at 4473.869 kb on + strand, within Psyr_3756 at 4473.870 kb on - strand, within Psyr_3756 at 4473.870 kb on - strand, within Psyr_3756 at 4473.870 kb on - strand, within Psyr_3756 at 4473.870 kb on - strand, within Psyr_3756 at 4473.885 kb on + strand, within Psyr_3756 at 4473.911 kb on + strand, within Psyr_3756 at 4473.911 kb on + strand, within Psyr_3756 at 4473.911 kb on + strand, within Psyr_3756 at 4473.911 kb on + strand, within Psyr_3756 at 4473.911 kb on + strand, within Psyr_3756 at 4473.912 kb on - strand, within Psyr_3756 at 4473.912 kb on - strand, within Psyr_3756 at 4473.912 kb on - strand, within Psyr_3756 at 4473.912 kb on - strand, within Psyr_3756 at 4473.912 kb on - strand, within Psyr_3756 at 4473.912 kb on - strand, within Psyr_3756 at 4473.912 kb on - strand, within Psyr_3756 at 4473.912 kb on - strand, within Psyr_3756 at 4473.912 kb on - strand, within Psyr_3756 at 4473.968 kb on + strand, within Psyr_3756 at 4473.968 kb on + strand, within Psyr_3756 at 4473.968 kb on + strand, within Psyr_3756 at 4473.969 kb on - strand, within Psyr_3756 at 4473.971 kb on + strand, within Psyr_3756 at 4473.971 kb on + strand, within Psyr_3756 at 4473.971 kb on + strand, within Psyr_3756 at 4473.972 kb on - strand, within Psyr_3756 at 4473.972 kb on - strand, within Psyr_3756 at 4474.008 kb on - strand at 4474.016 kb on + strand at 4474.016 kb on + strand at 4474.016 kb on + strand at 4474.016 kb on + strand at 4474.017 kb on - strand at 4474.017 kb on - strand at 4474.017 kb on - strand at 4474.017 kb on - strand at 4474.017 kb on - strand at 4474.017 kb on - strand at 4474.228 kb on + strand, within Psyr_3757 at 4474.228 kb on + strand, within Psyr_3757 at 4474.228 kb on + strand, within Psyr_3757 at 4474.228 kb on + strand, within Psyr_3757 at 4474.228 kb on + strand, within Psyr_3757 at 4474.228 kb on + strand, within Psyr_3757 at 4474.228 kb on + strand, within Psyr_3757 at 4474.228 kb on + strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.229 kb on - strand, within Psyr_3757 at 4474.242 kb on + strand, within Psyr_3757 at 4474.290 kb on - strand, within Psyr_3757 at 4474.308 kb on + strand, within Psyr_3757 at 4474.308 kb on + strand, within Psyr_3757 at 4474.308 kb on + strand, within Psyr_3757 at 4474.309 kb on - strand, within Psyr_3757 at 4474.309 kb on - strand, within Psyr_3757 at 4474.309 kb on - strand, within Psyr_3757 at 4474.363 kb on + strand, within Psyr_3757 at 4474.364 kb on - strand, within Psyr_3757 at 4474.456 kb on + strand at 4474.456 kb on + strand at 4474.456 kb on + strand at 4474.456 kb on + strand at 4474.456 kb on + strand at 4474.456 kb on + strand at 4474.456 kb on + strand at 4474.457 kb on - strand at 4474.508 kb on + strand at 4474.509 kb on - strand at 4474.519 kb on - strand at 4474.550 kb on + strand at 4474.550 kb on + strand at 4474.550 kb on + strand at 4474.551 kb on - strand at 4474.551 kb on - strand at 4474.551 kb on - strand at 4474.551 kb on - strand at 4474.553 kb on + strand at 4474.554 kb on - strand at 4474.554 kb on - strand at 4474.556 kb on + strand at 4474.556 kb on + strand at 4474.556 kb on + strand at 4474.557 kb on - strand at 4474.567 kb on + strand at 4474.567 kb on + strand at 4474.567 kb on + strand at 4474.568 kb on - strand at 4474.573 kb on + strand at 4474.573 kb on + strand at 4474.573 kb on + strand at 4474.573 kb on + strand at 4474.574 kb on - strand at 4474.574 kb on - strand at 4474.574 kb on - strand at 4474.574 kb on - strand at 4474.574 kb on - strand at 4474.574 kb on - strand at 4474.574 kb on - strand at 4474.574 kb on - strand at 4474.574 kb on - strand at 4474.577 kb on + strand at 4474.577 kb on + strand at 4474.577 kb on + strand at 4474.577 kb on + strand at 4474.578 kb on - strand at 4474.578 kb on - strand at 4474.578 kb on - strand at 4474.578 kb on - strand at 4474.578 kb on - strand at 4474.708 kb on + strand at 4474.708 kb on + strand at 4474.921 kb on + strand, within Psyr_3758 at 4475.069 kb on + strand, within Psyr_3758 at 4475.069 kb on + strand at 4475.070 kb on - strand, within Psyr_3758 at 4475.071 kb on + strand, within Psyr_3758 at 4475.072 kb on - strand, within Psyr_3758
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 4,471,559 + +0.7 4,471,559 + -0.6 4,471,580 + Psyr_3754 0.12 +0.3 4,471,580 + Psyr_3754 0.12 +0.6 4,471,580 + Psyr_3754 0.12 -1.4 4,471,580 + Psyr_3754 0.12 +0.1 4,471,627 + Psyr_3754 0.24 -0.0 4,471,670 + Psyr_3754 0.36 -1.3 4,471,670 + Psyr_3754 0.36 +0.7 4,471,695 - Psyr_3754 0.42 +0.5 4,471,751 + Psyr_3754 0.57 +0.1 4,471,751 + Psyr_3754 0.57 -0.2 4,471,752 - Psyr_3754 0.57 +0.2 4,471,828 + Psyr_3754 0.77 +0.3 4,471,867 + Psyr_3754 0.87 -0.2 4,471,867 + Psyr_3754 0.87 +0.3 4,471,867 + Psyr_3754 0.87 -0.1 4,471,868 - Psyr_3754 0.88 -0.2 4,471,868 - Psyr_3754 0.88 -1.3 4,472,196 + +0.4 4,472,201 - -1.1 4,472,201 - -0.1 4,472,207 - -0.9 4,472,207 - +0.5 4,472,211 + -0.6 4,472,212 - +1.3 4,472,248 + Psyr_3755 0.13 +0.6 4,472,272 - Psyr_3755 0.28 -0.7 4,472,315 + Psyr_3755 0.55 +0.9 4,472,382 + -1.0 4,472,382 + +0.1 4,472,382 + +0.6 4,472,382 + -0.1 4,472,383 - +0.5 4,472,411 + +0.6 4,472,412 - +2.0 4,472,412 - -0.4 4,472,412 - +0.2 4,472,413 + +0.3 4,472,413 + -0.8 4,472,413 + +1.3 4,472,413 + +0.4 4,472,413 + -0.5 4,472,413 + -0.5 4,472,414 - -0.3 4,472,414 - -0.6 4,472,414 - -0.4 4,472,421 - -0.9 4,472,507 + -0.4 4,472,508 - -0.3 4,472,508 - +0.3 4,472,565 - +0.1 4,472,625 + -2.0 4,472,625 + -0.1 4,472,630 - -0.4 4,472,630 - -0.7 4,472,801 + Psyr_3756 0.18 +0.2 4,472,801 + Psyr_3756 0.18 -0.3 4,472,801 + Psyr_3756 0.18 -0.6 4,472,801 + Psyr_3756 0.18 +1.3 4,472,802 - Psyr_3756 0.18 -0.3 4,472,996 + Psyr_3756 0.30 -1.3 4,472,996 + Psyr_3756 0.30 -0.7 4,472,996 + Psyr_3756 0.30 -1.6 4,472,996 + Psyr_3756 0.30 -1.1 4,472,996 + Psyr_3756 0.30 +1.3 4,472,996 + Psyr_3756 0.30 +0.2 4,472,997 - Psyr_3756 0.30 +0.7 4,472,997 - Psyr_3756 0.30 -1.8 4,472,997 - Psyr_3756 0.30 +1.2 4,472,997 - Psyr_3756 0.30 +0.7 4,472,997 - Psyr_3756 0.30 +0.4 4,472,997 - Psyr_3756 0.30 -0.4 4,473,233 + Psyr_3756 0.44 -0.3 4,473,233 + Psyr_3756 0.44 -0.7 4,473,233 + Psyr_3756 0.44 +0.9 4,473,234 - Psyr_3756 0.44 +1.3 4,473,239 + Psyr_3756 0.44 +0.7 4,473,240 - Psyr_3756 0.44 -1.1 4,473,261 - Psyr_3756 0.46 +1.3 4,473,261 - Psyr_3756 0.46 -0.1 4,473,575 + Psyr_3756 0.64 +0.6 4,473,575 + Psyr_3756 0.64 -0.3 4,473,575 + Psyr_3756 0.64 +0.2 4,473,575 + Psyr_3756 0.64 +0.7 4,473,575 + Psyr_3756 0.64 +0.9 4,473,576 - Psyr_3756 0.64 -0.6 4,473,576 - Psyr_3756 0.64 +0.2 4,473,576 - Psyr_3756 0.64 -0.3 4,473,576 - Psyr_3756 0.64 +0.3 4,473,576 - Psyr_3756 0.64 -0.2 4,473,767 + Psyr_3756 0.76 +0.1 4,473,767 + Psyr_3756 0.76 +0.8 4,473,767 + Psyr_3756 0.76 +0.6 4,473,767 + Psyr_3756 0.76 +0.9 4,473,767 + Psyr_3756 0.76 -0.4 4,473,767 + Psyr_3756 0.76 +0.3 4,473,767 + Psyr_3756 0.76 -0.0 4,473,767 + Psyr_3756 0.76 -0.6 4,473,767 + Psyr_3756 0.76 +0.6 4,473,767 + Psyr_3756 0.76 +0.8 4,473,768 - Psyr_3756 0.76 +1.3 4,473,768 - Psyr_3756 0.76 +0.6 4,473,768 - -0.0 4,473,768 - Psyr_3756 0.76 +0.3 4,473,824 - Psyr_3756 0.79 +0.5 4,473,842 + Psyr_3756 0.81 -0.3 4,473,843 - Psyr_3756 0.81 -0.7 4,473,843 - Psyr_3756 0.81 -2.5 4,473,869 + Psyr_3756 0.82 +0.3 4,473,870 - Psyr_3756 0.82 -1.6 4,473,870 - Psyr_3756 0.82 -0.3 4,473,870 - Psyr_3756 0.82 +0.5 4,473,870 - Psyr_3756 0.82 +0.6 4,473,885 + Psyr_3756 0.83 +1.5 4,473,911 + Psyr_3756 0.85 -1.3 4,473,911 + Psyr_3756 0.85 +0.6 4,473,911 + Psyr_3756 0.85 +0.3 4,473,911 + Psyr_3756 0.85 -0.3 4,473,911 + Psyr_3756 0.85 -0.2 4,473,912 - Psyr_3756 0.85 +1.3 4,473,912 - Psyr_3756 0.85 +0.3 4,473,912 - Psyr_3756 0.85 -0.0 4,473,912 - Psyr_3756 0.85 +0.8 4,473,912 - Psyr_3756 0.85 +0.1 4,473,912 - Psyr_3756 0.85 +0.9 4,473,912 - Psyr_3756 0.85 -0.5 4,473,912 - Psyr_3756 0.85 -1.6 4,473,912 - Psyr_3756 0.85 -0.0 4,473,968 + Psyr_3756 0.88 +0.3 4,473,968 + Psyr_3756 0.88 +2.3 4,473,968 + Psyr_3756 0.88 -0.5 4,473,969 - Psyr_3756 0.88 -1.6 4,473,971 + Psyr_3756 0.88 +0.5 4,473,971 + Psyr_3756 0.88 -0.4 4,473,971 + Psyr_3756 0.88 +0.4 4,473,972 - Psyr_3756 0.88 +2.0 4,473,972 - Psyr_3756 0.88 +1.2 4,474,008 - +0.2 4,474,016 + -0.4 4,474,016 + -0.7 4,474,016 + +0.7 4,474,016 + -0.4 4,474,017 - -0.3 4,474,017 - +1.4 4,474,017 - +0.2 4,474,017 - -0.3 4,474,017 - +1.3 4,474,017 - -0.2 4,474,228 + Psyr_3757 0.21 +0.2 4,474,228 + Psyr_3757 0.21 +0.5 4,474,228 + Psyr_3757 0.21 +0.3 4,474,228 + Psyr_3757 0.21 +1.3 4,474,228 + Psyr_3757 0.21 +0.4 4,474,228 + Psyr_3757 0.21 -0.2 4,474,228 + Psyr_3757 0.21 -0.0 4,474,228 + Psyr_3757 0.21 +0.9 4,474,229 - Psyr_3757 0.22 -0.8 4,474,229 - Psyr_3757 0.22 -0.3 4,474,229 - Psyr_3757 0.22 -0.1 4,474,229 - Psyr_3757 0.22 -0.3 4,474,229 - Psyr_3757 0.22 +1.0 4,474,229 - Psyr_3757 0.22 -0.9 4,474,229 - Psyr_3757 0.22 +0.3 4,474,229 - Psyr_3757 0.22 -0.4 4,474,229 - Psyr_3757 0.22 -0.5 4,474,229 - Psyr_3757 0.22 -0.3 4,474,229 - Psyr_3757 0.22 +0.8 4,474,229 - Psyr_3757 0.22 +0.1 4,474,229 - Psyr_3757 0.22 +1.0 4,474,242 + Psyr_3757 0.26 +1.1 4,474,290 - Psyr_3757 0.41 -0.1 4,474,308 + Psyr_3757 0.47 -1.1 4,474,308 + Psyr_3757 0.47 +1.2 4,474,308 + Psyr_3757 0.47 +1.0 4,474,309 - Psyr_3757 0.47 +0.3 4,474,309 - Psyr_3757 0.47 +2.0 4,474,309 - Psyr_3757 0.47 +0.1 4,474,363 + Psyr_3757 0.65 -0.4 4,474,364 - Psyr_3757 0.65 -0.5 4,474,456 + -0.4 4,474,456 + +1.3 4,474,456 + +1.5 4,474,456 + +0.5 4,474,456 + -0.3 4,474,456 + -1.5 4,474,456 + -0.7 4,474,457 - +1.6 4,474,508 + -0.0 4,474,509 - +1.7 4,474,519 - +0.9 4,474,550 + +0.0 4,474,550 + +0.1 4,474,550 + +0.5 4,474,551 - +0.1 4,474,551 - -0.3 4,474,551 - +0.7 4,474,551 - -0.0 4,474,553 + -0.4 4,474,554 - -0.3 4,474,554 - +1.4 4,474,556 + -0.4 4,474,556 + -0.2 4,474,556 + -0.2 4,474,557 - +0.1 4,474,567 + +0.2 4,474,567 + +0.5 4,474,567 + -0.4 4,474,568 - -0.0 4,474,573 + +0.5 4,474,573 + -0.2 4,474,573 + -1.8 4,474,573 + +0.7 4,474,574 - -0.7 4,474,574 - -1.5 4,474,574 - +0.8 4,474,574 - -0.3 4,474,574 - +0.1 4,474,574 - +1.0 4,474,574 - -0.6 4,474,574 - +0.2 4,474,574 - +0.6 4,474,577 + -0.8 4,474,577 + -0.4 4,474,577 + -0.4 4,474,577 + -0.9 4,474,578 - -0.0 4,474,578 - -1.6 4,474,578 - +1.4 4,474,578 - +0.3 4,474,578 - -1.2 4,474,708 + +1.2 4,474,708 + -0.0 4,474,921 + Psyr_3758 0.25 +1.3 4,475,069 + Psyr_3758 0.42 +0.5 4,475,069 + +1.3 4,475,070 - Psyr_3758 0.42 +0.1 4,475,071 + Psyr_3758 0.43 -1.7 4,475,072 - Psyr_3758 0.43 +0.2
Or see this region's nucleotide sequence