Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3078 and Psyr_3079 are separated by 73 nucleotides Psyr_3079 and Psyr_3080 are separated by 15 nucleotides Psyr_3080 and Psyr_3081 overlap by 4 nucleotides
Psyr_3078: Psyr_3078 - Peptidase M20:Peptidase M20, at 3,682,286 to 3,683,527
_3078
Psyr_3079: Psyr_3079 - CDS, at 3,683,601 to 3,684,083
_3079
Psyr_3080: Psyr_3080 - CDS, at 3,684,099 to 3,686,681
_3080
Psyr_3081: Psyr_3081 - Secretion protein HlyD, at 3,686,678 to 3,687,811
_3081
Position (kb)
3684
3685
3686
3687 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3683.140 kb on + strand, within Psyr_3078 at 3683.342 kb on + strand, within Psyr_3078 at 3683.343 kb on - strand, within Psyr_3078 at 3683.346 kb on + strand, within Psyr_3078 at 3683.406 kb on + strand at 3683.406 kb on + strand at 3683.406 kb on + strand at 3683.406 kb on + strand at 3683.407 kb on - strand at 3683.407 kb on - strand at 3683.407 kb on - strand at 3683.407 kb on - strand at 3683.407 kb on - strand at 3683.572 kb on + strand at 3683.572 kb on + strand at 3683.572 kb on + strand at 3683.572 kb on + strand at 3683.573 kb on - strand at 3683.573 kb on - strand at 3683.573 kb on - strand at 3684.085 kb on + strand at 3684.085 kb on + strand at 3684.085 kb on + strand at 3684.085 kb on + strand at 3684.086 kb on - strand at 3684.086 kb on - strand at 3684.086 kb on - strand at 3684.086 kb on - strand at 3684.101 kb on - strand at 3684.101 kb on - strand at 3684.123 kb on + strand at 3684.123 kb on + strand at 3684.124 kb on - strand at 3684.124 kb on - strand at 3684.149 kb on - strand at 3684.257 kb on + strand at 3684.518 kb on - strand, within Psyr_3080 at 3684.621 kb on - strand, within Psyr_3080 at 3684.805 kb on + strand, within Psyr_3080 at 3684.806 kb on - strand, within Psyr_3080 at 3684.874 kb on + strand, within Psyr_3080 at 3684.875 kb on - strand, within Psyr_3080 at 3685.133 kb on - strand, within Psyr_3080 at 3685.133 kb on - strand, within Psyr_3080 at 3685.148 kb on - strand, within Psyr_3080 at 3685.148 kb on - strand, within Psyr_3080 at 3685.225 kb on + strand, within Psyr_3080 at 3685.681 kb on + strand, within Psyr_3080 at 3685.681 kb on + strand, within Psyr_3080 at 3685.681 kb on + strand, within Psyr_3080 at 3685.681 kb on + strand, within Psyr_3080 at 3685.681 kb on + strand, within Psyr_3080 at 3685.682 kb on - strand, within Psyr_3080 at 3685.682 kb on - strand, within Psyr_3080 at 3685.682 kb on - strand, within Psyr_3080 at 3685.682 kb on - strand, within Psyr_3080 at 3685.682 kb on - strand, within Psyr_3080 at 3685.682 kb on - strand, within Psyr_3080 at 3685.682 kb on - strand, within Psyr_3080 at 3685.734 kb on + strand, within Psyr_3080 at 3685.735 kb on - strand, within Psyr_3080 at 3685.739 kb on + strand, within Psyr_3080 at 3685.862 kb on - strand, within Psyr_3080 at 3685.862 kb on - strand, within Psyr_3080 at 3686.131 kb on + strand, within Psyr_3080 at 3686.131 kb on + strand, within Psyr_3080 at 3686.131 kb on + strand, within Psyr_3080 at 3686.131 kb on + strand, within Psyr_3080 at 3686.131 kb on + strand, within Psyr_3080 at 3686.132 kb on - strand, within Psyr_3080 at 3686.132 kb on - strand, within Psyr_3080 at 3686.132 kb on - strand, within Psyr_3080 at 3686.132 kb on - strand, within Psyr_3080 at 3686.132 kb on - strand, within Psyr_3080 at 3686.133 kb on + strand, within Psyr_3080 at 3686.133 kb on + strand, within Psyr_3080 at 3686.133 kb on + strand, within Psyr_3080 at 3686.134 kb on - strand, within Psyr_3080 at 3686.134 kb on - strand, within Psyr_3080 at 3686.134 kb on - strand, within Psyr_3080 at 3686.472 kb on - strand at 3686.472 kb on - strand at 3686.672 kb on + strand at 3686.673 kb on - strand at 3686.673 kb on - strand at 3686.821 kb on + strand at 3687.423 kb on + strand, within Psyr_3081 at 3687.427 kb on + strand, within Psyr_3081 at 3687.427 kb on + strand, within Psyr_3081 at 3687.428 kb on - strand, within Psyr_3081 at 3687.428 kb on - strand, within Psyr_3081
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 3,683,140 + Psyr_3078 0.69 +0.1 3,683,342 + Psyr_3078 0.85 -0.8 3,683,343 - Psyr_3078 0.85 +0.5 3,683,346 + Psyr_3078 0.85 -0.8 3,683,406 + +1.1 3,683,406 + -0.5 3,683,406 + +0.8 3,683,406 + +0.5 3,683,407 - -0.1 3,683,407 - +0.4 3,683,407 - +0.7 3,683,407 - +1.3 3,683,407 - +0.1 3,683,572 + +1.0 3,683,572 + +0.7 3,683,572 + -0.4 3,683,572 + +0.5 3,683,573 - +0.3 3,683,573 - -1.8 3,683,573 - -0.6 3,684,085 + +1.9 3,684,085 + +0.2 3,684,085 + -0.1 3,684,085 + +1.6 3,684,086 - +0.9 3,684,086 - -0.8 3,684,086 - +0.2 3,684,086 - +1.1 3,684,101 - +1.3 3,684,101 - -0.9 3,684,123 + +0.8 3,684,123 + +0.2 3,684,124 - +1.6 3,684,124 - -0.4 3,684,149 - -1.1 3,684,257 + +0.3 3,684,518 - Psyr_3080 0.16 +0.8 3,684,621 - Psyr_3080 0.20 -0.9 3,684,805 + Psyr_3080 0.27 +0.6 3,684,806 - Psyr_3080 0.27 +0.4 3,684,874 + Psyr_3080 0.30 +0.2 3,684,875 - Psyr_3080 0.30 +0.6 3,685,133 - Psyr_3080 0.40 +0.1 3,685,133 - Psyr_3080 0.40 +0.6 3,685,148 - Psyr_3080 0.41 +0.5 3,685,148 - Psyr_3080 0.41 -0.6 3,685,225 + Psyr_3080 0.44 +0.6 3,685,681 + Psyr_3080 0.61 -1.4 3,685,681 + Psyr_3080 0.61 -0.6 3,685,681 + Psyr_3080 0.61 +0.2 3,685,681 + Psyr_3080 0.61 +0.1 3,685,681 + Psyr_3080 0.61 -0.3 3,685,682 - Psyr_3080 0.61 +1.9 3,685,682 - Psyr_3080 0.61 +0.2 3,685,682 - Psyr_3080 0.61 +0.1 3,685,682 - Psyr_3080 0.61 +0.1 3,685,682 - Psyr_3080 0.61 +0.2 3,685,682 - Psyr_3080 0.61 +1.8 3,685,682 - Psyr_3080 0.61 -3.0 3,685,734 + Psyr_3080 0.63 +0.9 3,685,735 - Psyr_3080 0.63 -0.8 3,685,739 + Psyr_3080 0.63 -0.4 3,685,862 - Psyr_3080 0.68 +1.2 3,685,862 - Psyr_3080 0.68 +1.4 3,686,131 + Psyr_3080 0.79 +0.7 3,686,131 + Psyr_3080 0.79 -0.2 3,686,131 + Psyr_3080 0.79 -1.1 3,686,131 + Psyr_3080 0.79 -0.6 3,686,131 + Psyr_3080 0.79 +2.0 3,686,132 - Psyr_3080 0.79 +1.1 3,686,132 - Psyr_3080 0.79 -0.1 3,686,132 - Psyr_3080 0.79 +0.2 3,686,132 - Psyr_3080 0.79 -0.1 3,686,132 - Psyr_3080 0.79 -0.1 3,686,133 + Psyr_3080 0.79 +0.3 3,686,133 + Psyr_3080 0.79 +0.8 3,686,133 + Psyr_3080 0.79 +0.4 3,686,134 - Psyr_3080 0.79 -1.1 3,686,134 - Psyr_3080 0.79 +0.2 3,686,134 - Psyr_3080 0.79 +0.4 3,686,472 - -0.9 3,686,472 - -0.7 3,686,672 + +0.4 3,686,673 - +0.5 3,686,673 - +0.0 3,686,821 + +1.2 3,687,423 + Psyr_3081 0.66 +0.4 3,687,427 + Psyr_3081 0.66 -0.7 3,687,427 + Psyr_3081 0.66 +0.7 3,687,428 - Psyr_3081 0.66 +0.5 3,687,428 - Psyr_3081 0.66 +1.2
Or see this region's nucleotide sequence