Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3052 and Psyr_3053 overlap by 4 nucleotides Psyr_3053 and Psyr_3054 are separated by 146 nucleotides Psyr_3054 and Psyr_3055 overlap by 4 nucleotides Psyr_3055 and Psyr_3056 are separated by 10 nucleotides
Psyr_3052: Psyr_3052 - Na+/solute symporter, at 3,649,676 to 3,651,205
_3052
Psyr_3053: Psyr_3053 - conserved hypothetical protein, at 3,651,202 to 3,651,462
_3053
Psyr_3054: Psyr_3054 - Choline/ethanolamine kinase:Aminoglycoside phosphotransferase, at 3,651,609 to 3,652,544
_3054
Psyr_3055: Psyr_3055 - Aminoglycoside phosphotransferase, at 3,652,541 to 3,653,494
_3055
Psyr_3056: Psyr_3056 - Amidase, hydantoinase/carbamoylase, at 3,653,505 to 3,654,785
_3056
Position (kb)
3651
3652
3653 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3650.622 kb on + strand, within Psyr_3052 at 3650.622 kb on + strand, within Psyr_3052 at 3650.622 kb on + strand, within Psyr_3052 at 3650.622 kb on + strand, within Psyr_3052 at 3650.622 kb on + strand, within Psyr_3052 at 3650.622 kb on + strand, within Psyr_3052 at 3650.622 kb on + strand, within Psyr_3052 at 3650.622 kb on + strand, within Psyr_3052 at 3650.623 kb on - strand, within Psyr_3052 at 3650.623 kb on - strand, within Psyr_3052 at 3650.623 kb on - strand, within Psyr_3052 at 3650.623 kb on - strand, within Psyr_3052 at 3650.623 kb on - strand, within Psyr_3052 at 3650.623 kb on - strand, within Psyr_3052 at 3650.623 kb on - strand, within Psyr_3052 at 3650.623 kb on - strand, within Psyr_3052 at 3650.623 kb on - strand, within Psyr_3052 at 3650.922 kb on + strand, within Psyr_3052 at 3650.923 kb on - strand, within Psyr_3052 at 3650.923 kb on - strand, within Psyr_3052 at 3650.923 kb on - strand, within Psyr_3052 at 3650.950 kb on - strand, within Psyr_3052 at 3651.120 kb on + strand at 3651.174 kb on + strand at 3651.174 kb on + strand at 3651.175 kb on - strand at 3651.175 kb on - strand at 3651.254 kb on + strand, within Psyr_3053 at 3651.254 kb on + strand, within Psyr_3053 at 3651.254 kb on + strand, within Psyr_3053 at 3651.255 kb on - strand, within Psyr_3053 at 3651.255 kb on - strand, within Psyr_3053 at 3651.401 kb on + strand, within Psyr_3053 at 3651.401 kb on + strand, within Psyr_3053 at 3651.402 kb on - strand, within Psyr_3053 at 3651.402 kb on - strand, within Psyr_3053 at 3651.498 kb on + strand at 3651.559 kb on + strand at 3651.568 kb on - strand at 3651.568 kb on - strand at 3651.686 kb on - strand at 3651.691 kb on + strand at 3651.692 kb on - strand at 3651.814 kb on + strand, within Psyr_3054 at 3651.815 kb on - strand, within Psyr_3054 at 3651.815 kb on - strand, within Psyr_3054 at 3651.815 kb on - strand, within Psyr_3054 at 3651.907 kb on + strand, within Psyr_3054 at 3652.030 kb on + strand, within Psyr_3054 at 3652.185 kb on + strand, within Psyr_3054 at 3652.185 kb on + strand, within Psyr_3054 at 3652.185 kb on + strand, within Psyr_3054 at 3652.185 kb on + strand, within Psyr_3054 at 3652.185 kb on + strand, within Psyr_3054 at 3652.186 kb on - strand, within Psyr_3054 at 3652.382 kb on - strand, within Psyr_3054 at 3652.382 kb on - strand, within Psyr_3054 at 3652.382 kb on - strand, within Psyr_3054 at 3652.382 kb on - strand, within Psyr_3054 at 3652.451 kb on - strand at 3652.716 kb on + strand, within Psyr_3055 at 3652.855 kb on - strand, within Psyr_3055 at 3653.328 kb on + strand, within Psyr_3055 at 3653.329 kb on - strand, within Psyr_3055 at 3653.487 kb on + strand at 3653.488 kb on - strand at 3653.488 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 3,650,622 + Psyr_3052 0.62 +0.3 3,650,622 + Psyr_3052 0.62 +0.1 3,650,622 + Psyr_3052 0.62 +0.0 3,650,622 + Psyr_3052 0.62 -0.6 3,650,622 + Psyr_3052 0.62 +0.0 3,650,622 + Psyr_3052 0.62 -0.5 3,650,622 + Psyr_3052 0.62 +0.2 3,650,622 + Psyr_3052 0.62 +0.4 3,650,623 - Psyr_3052 0.62 +1.6 3,650,623 - Psyr_3052 0.62 +1.2 3,650,623 - Psyr_3052 0.62 +0.0 3,650,623 - Psyr_3052 0.62 +0.7 3,650,623 - Psyr_3052 0.62 +0.6 3,650,623 - Psyr_3052 0.62 -0.0 3,650,623 - Psyr_3052 0.62 +1.5 3,650,623 - Psyr_3052 0.62 +0.5 3,650,623 - Psyr_3052 0.62 -0.2 3,650,922 + Psyr_3052 0.81 -0.3 3,650,923 - Psyr_3052 0.82 -0.2 3,650,923 - Psyr_3052 0.82 +0.9 3,650,923 - Psyr_3052 0.82 -0.1 3,650,950 - Psyr_3052 0.83 -0.3 3,651,120 + -0.5 3,651,174 + -1.3 3,651,174 + -0.4 3,651,175 - -0.2 3,651,175 - -0.2 3,651,254 + Psyr_3053 0.20 -0.8 3,651,254 + Psyr_3053 0.20 +0.5 3,651,254 + Psyr_3053 0.20 +0.4 3,651,255 - Psyr_3053 0.20 +1.0 3,651,255 - Psyr_3053 0.20 -0.4 3,651,401 + Psyr_3053 0.76 +0.4 3,651,401 + Psyr_3053 0.76 -2.5 3,651,402 - Psyr_3053 0.77 +0.7 3,651,402 - Psyr_3053 0.77 +0.8 3,651,498 + +0.8 3,651,559 + +0.3 3,651,568 - +2.2 3,651,568 - +1.0 3,651,686 - -1.1 3,651,691 + -0.0 3,651,692 - -1.3 3,651,814 + Psyr_3054 0.22 -1.1 3,651,815 - Psyr_3054 0.22 -0.6 3,651,815 - Psyr_3054 0.22 -1.8 3,651,815 - Psyr_3054 0.22 -0.4 3,651,907 + Psyr_3054 0.32 +1.2 3,652,030 + Psyr_3054 0.45 -0.2 3,652,185 + Psyr_3054 0.62 -0.0 3,652,185 + Psyr_3054 0.62 -2.1 3,652,185 + Psyr_3054 0.62 +0.0 3,652,185 + Psyr_3054 0.62 -0.7 3,652,185 + Psyr_3054 0.62 -1.2 3,652,186 - Psyr_3054 0.62 -0.9 3,652,382 - Psyr_3054 0.83 -0.0 3,652,382 - Psyr_3054 0.83 -1.0 3,652,382 - Psyr_3054 0.83 +0.7 3,652,382 - Psyr_3054 0.83 +0.2 3,652,451 - -0.1 3,652,716 + Psyr_3055 0.18 +2.2 3,652,855 - Psyr_3055 0.33 -2.1 3,653,328 + Psyr_3055 0.82 +0.3 3,653,329 - Psyr_3055 0.83 +1.7 3,653,487 + -1.2 3,653,488 - +1.2 3,653,488 - -0.8
Or see this region's nucleotide sequence