Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2766 and Psyr_2767 overlap by 4 nucleotides Psyr_2767 and Psyr_2768 are separated by 295 nucleotides Psyr_2768 and Psyr_2769 are separated by 13 nucleotides Psyr_2769 and Psyr_2770 overlap by 1 nucleotides
Psyr_2766: Psyr_2766 - conserved hypothetical protein, at 3,352,726 to 3,353,253
_2766
Psyr_2767: Psyr_2767 - Glycoside hydrolase, family 19, at 3,353,250 to 3,353,783
_2767
Psyr_2768: Psyr_2768 - tail fiber assembly domain protein, at 3,354,079 to 3,354,447
_2768
Psyr_2769: Psyr_2769 - conserved hypothetical protein, at 3,354,461 to 3,354,775
_2769
Psyr_2770: Psyr_2770 - tail fiber domain protein, at 3,354,775 to 3,355,878
_2770
Position (kb)
3354
3355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3353.186 kb on + strand, within Psyr_2766 at 3353.278 kb on + strand at 3353.480 kb on - strand, within Psyr_2767 at 3353.546 kb on + strand, within Psyr_2767 at 3353.686 kb on + strand, within Psyr_2767 at 3353.687 kb on - strand, within Psyr_2767 at 3353.777 kb on + strand at 3353.778 kb on - strand at 3353.807 kb on + strand at 3353.808 kb on - strand at 3353.844 kb on + strand at 3353.844 kb on + strand at 3353.845 kb on - strand at 3353.845 kb on - strand at 3353.863 kb on + strand at 3353.863 kb on + strand at 3353.864 kb on - strand at 3353.874 kb on + strand at 3353.874 kb on + strand at 3353.875 kb on - strand at 3353.889 kb on + strand at 3353.889 kb on + strand at 3353.943 kb on + strand at 3353.943 kb on + strand at 3353.943 kb on + strand at 3353.948 kb on - strand at 3353.990 kb on + strand at 3353.990 kb on + strand at 3353.990 kb on + strand at 3353.991 kb on - strand at 3353.991 kb on - strand at 3353.991 kb on - strand at 3354.046 kb on + strand at 3354.315 kb on + strand, within Psyr_2768 at 3354.421 kb on + strand at 3354.422 kb on - strand at 3354.422 kb on - strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.448 kb on - strand at 3354.555 kb on + strand, within Psyr_2769 at 3354.555 kb on + strand, within Psyr_2769 at 3354.556 kb on - strand, within Psyr_2769 at 3354.556 kb on - strand, within Psyr_2769 at 3354.585 kb on - strand, within Psyr_2769 at 3354.610 kb on + strand, within Psyr_2769 at 3354.659 kb on - strand, within Psyr_2769 at 3354.691 kb on + strand, within Psyr_2769 at 3354.691 kb on + strand, within Psyr_2769 at 3354.728 kb on + strand, within Psyr_2769 at 3354.777 kb on - strand at 3354.798 kb on + strand at 3354.798 kb on + strand at 3354.798 kb on + strand at 3354.798 kb on + strand at 3354.799 kb on - strand at 3354.800 kb on + strand at 3354.800 kb on + strand at 3354.801 kb on - strand at 3354.801 kb on - strand at 3354.801 kb on - strand at 3354.870 kb on + strand at 3354.871 kb on - strand at 3354.896 kb on + strand, within Psyr_2770 at 3354.904 kb on - strand, within Psyr_2770 at 3354.904 kb on - strand, within Psyr_2770 at 3354.981 kb on - strand, within Psyr_2770 at 3355.096 kb on + strand, within Psyr_2770 at 3355.097 kb on - strand, within Psyr_2770 at 3355.160 kb on + strand, within Psyr_2770 at 3355.160 kb on + strand, within Psyr_2770 at 3355.161 kb on - strand, within Psyr_2770 at 3355.175 kb on - strand, within Psyr_2770 at 3355.297 kb on - strand, within Psyr_2770 at 3355.300 kb on + strand, within Psyr_2770 at 3355.301 kb on - strand, within Psyr_2770 at 3355.301 kb on - strand, within Psyr_2770 at 3355.324 kb on - strand, within Psyr_2770 at 3355.340 kb on - strand, within Psyr_2770 at 3355.395 kb on + strand, within Psyr_2770
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 3,353,186 + Psyr_2766 0.87 +0.4 3,353,278 + -1.0 3,353,480 - Psyr_2767 0.43 -0.0 3,353,546 + Psyr_2767 0.55 +0.7 3,353,686 + Psyr_2767 0.82 +0.4 3,353,687 - Psyr_2767 0.82 +1.7 3,353,777 + +1.4 3,353,778 - -0.1 3,353,807 + +1.7 3,353,808 - -0.7 3,353,844 + -0.8 3,353,844 + -1.0 3,353,845 - +0.3 3,353,845 - +0.0 3,353,863 + +1.5 3,353,863 + -0.6 3,353,864 - -1.6 3,353,874 + +0.7 3,353,874 + -0.7 3,353,875 - -0.9 3,353,889 + +1.0 3,353,889 + +0.3 3,353,943 + -0.3 3,353,943 + -1.2 3,353,943 + -0.8 3,353,948 - +0.3 3,353,990 + +1.3 3,353,990 + +0.6 3,353,990 + +0.5 3,353,991 - -2.9 3,353,991 - -0.3 3,353,991 - -1.4 3,354,046 + +0.1 3,354,315 + Psyr_2768 0.64 -2.0 3,354,421 + -0.5 3,354,422 - -1.0 3,354,422 - +0.1 3,354,447 + -0.7 3,354,447 + -0.8 3,354,447 + +0.3 3,354,447 + -0.0 3,354,447 + -0.6 3,354,447 + -0.8 3,354,448 - -0.6 3,354,555 + Psyr_2769 0.30 -0.5 3,354,555 + Psyr_2769 0.30 -1.5 3,354,556 - Psyr_2769 0.30 -1.1 3,354,556 - Psyr_2769 0.30 -1.2 3,354,585 - Psyr_2769 0.39 -1.5 3,354,610 + Psyr_2769 0.47 -0.7 3,354,659 - Psyr_2769 0.63 -1.3 3,354,691 + Psyr_2769 0.73 -2.2 3,354,691 + Psyr_2769 0.73 -2.5 3,354,728 + Psyr_2769 0.85 +3.6 3,354,777 - +0.9 3,354,798 + -1.2 3,354,798 + +0.3 3,354,798 + -0.3 3,354,798 + -1.4 3,354,799 - +2.3 3,354,800 + -0.5 3,354,800 + -2.4 3,354,801 - -0.6 3,354,801 - -0.9 3,354,801 - +0.6 3,354,870 + -1.0 3,354,871 - -0.0 3,354,896 + Psyr_2770 0.11 -1.5 3,354,904 - Psyr_2770 0.12 -1.0 3,354,904 - Psyr_2770 0.12 -1.5 3,354,981 - Psyr_2770 0.19 +0.0 3,355,096 + Psyr_2770 0.29 -0.8 3,355,097 - Psyr_2770 0.29 -0.0 3,355,160 + Psyr_2770 0.35 -0.1 3,355,160 + Psyr_2770 0.35 +0.3 3,355,161 - Psyr_2770 0.35 -1.1 3,355,175 - Psyr_2770 0.36 -0.9 3,355,297 - Psyr_2770 0.47 -0.0 3,355,300 + Psyr_2770 0.48 +0.8 3,355,301 - Psyr_2770 0.48 -1.0 3,355,301 - Psyr_2770 0.48 -0.2 3,355,324 - Psyr_2770 0.50 -0.4 3,355,340 - Psyr_2770 0.51 -1.0 3,355,395 + Psyr_2770 0.56 -0.3
Or see this region's nucleotide sequence