Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2511 and Psyr_2512 are separated by 10 nucleotides Psyr_2512 and Psyr_2513 are separated by 388 nucleotides Psyr_2513 and Psyr_2514 are separated by 6 nucleotides Psyr_2514 and Psyr_2515 are separated by 3 nucleotides
Psyr_2511: Psyr_2511 - UvrD/REP helicase, at 2,919,741 to 2,922,134
_2511
Psyr_2512: Psyr_2512 - conserved hypothetical protein, at 2,922,145 to 2,922,411
_2512
Psyr_2513: Psyr_2513 - extracellular solute-binding protein, family 1, at 2,922,800 to 2,923,879
_2513
Psyr_2514: Psyr_2514 - ABC transporter, at 2,923,886 to 2,924,599
_2514
Psyr_2515: Psyr_2515 - Binding-protein-dependent transport systems inner membrane component, at 2,924,603 to 2,925,451
_2515
Position (kb)
2922
2923
2924 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2921.808 kb on + strand, within Psyr_2511 at 2921.809 kb on - strand, within Psyr_2511 at 2921.809 kb on - strand, within Psyr_2511 at 2921.921 kb on + strand at 2921.979 kb on + strand at 2921.979 kb on + strand at 2921.980 kb on - strand at 2922.012 kb on + strand at 2922.018 kb on + strand at 2922.018 kb on + strand at 2922.018 kb on + strand at 2922.018 kb on + strand at 2922.019 kb on - strand at 2922.019 kb on - strand at 2922.019 kb on - strand at 2922.019 kb on - strand at 2922.019 kb on - strand at 2922.019 kb on - strand at 2922.019 kb on - strand at 2922.019 kb on - strand at 2922.174 kb on + strand, within Psyr_2512 at 2922.174 kb on + strand, within Psyr_2512 at 2922.584 kb on + strand at 2922.643 kb on + strand at 2922.646 kb on - strand at 2922.661 kb on - strand at 2922.667 kb on + strand at 2922.670 kb on + strand at 2922.671 kb on - strand at 2922.673 kb on + strand at 2922.674 kb on - strand at 2922.674 kb on - strand at 2922.869 kb on - strand at 2922.872 kb on + strand at 2922.872 kb on + strand at 2922.872 kb on + strand at 2922.873 kb on - strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.888 kb on - strand at 2922.888 kb on - strand at 2922.888 kb on - strand at 2922.888 kb on - strand at 2922.889 kb on + strand at 2922.889 kb on + strand at 2922.889 kb on + strand at 2922.889 kb on + strand at 2922.889 kb on + strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.891 kb on + strand at 2922.891 kb on + strand at 2922.891 kb on + strand at 2922.892 kb on - strand at 2922.892 kb on - strand at 2922.945 kb on - strand, within Psyr_2513 at 2922.945 kb on - strand, within Psyr_2513 at 2923.166 kb on + strand, within Psyr_2513 at 2923.167 kb on - strand, within Psyr_2513 at 2923.302 kb on + strand, within Psyr_2513 at 2923.302 kb on + strand, within Psyr_2513 at 2923.303 kb on - strand, within Psyr_2513 at 2923.346 kb on + strand, within Psyr_2513 at 2923.346 kb on + strand, within Psyr_2513 at 2923.346 kb on + strand, within Psyr_2513 at 2923.347 kb on - strand, within Psyr_2513 at 2923.385 kb on + strand, within Psyr_2513 at 2923.424 kb on + strand, within Psyr_2513 at 2923.424 kb on + strand, within Psyr_2513 at 2923.451 kb on + strand, within Psyr_2513 at 2923.452 kb on - strand, within Psyr_2513 at 2923.562 kb on + strand, within Psyr_2513 at 2923.658 kb on + strand, within Psyr_2513 at 2923.659 kb on - strand, within Psyr_2513 at 2923.707 kb on - strand, within Psyr_2513 at 2923.707 kb on - strand, within Psyr_2513 at 2923.773 kb on - strand at 2923.985 kb on + strand, within Psyr_2514 at 2924.043 kb on + strand, within Psyr_2514 at 2924.043 kb on + strand, within Psyr_2514 at 2924.043 kb on + strand, within Psyr_2514 at 2924.044 kb on - strand, within Psyr_2514 at 2924.044 kb on - strand, within Psyr_2514 at 2924.044 kb on - strand, within Psyr_2514 at 2924.044 kb on - strand, within Psyr_2514 at 2924.314 kb on - strand, within Psyr_2514 at 2924.338 kb on + strand, within Psyr_2514 at 2924.414 kb on + strand, within Psyr_2514 at 2924.545 kb on + strand at 2924.576 kb on + strand at 2924.576 kb on + strand at 2924.577 kb on - strand at 2924.577 kb on - strand at 2924.577 kb on - strand at 2924.577 kb on - strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 2,921,808 + Psyr_2511 0.86 -0.1 2,921,809 - Psyr_2511 0.86 +1.4 2,921,809 - Psyr_2511 0.86 +0.6 2,921,921 + +1.1 2,921,979 + -0.2 2,921,979 + +1.2 2,921,980 - +0.2 2,922,012 + +1.9 2,922,018 + -0.8 2,922,018 + +2.4 2,922,018 + -0.3 2,922,018 + -0.2 2,922,019 - -0.9 2,922,019 - +1.4 2,922,019 - +0.4 2,922,019 - +1.6 2,922,019 - -0.5 2,922,019 - +0.8 2,922,019 - +0.8 2,922,019 - +0.3 2,922,174 + Psyr_2512 0.11 -0.5 2,922,174 + Psyr_2512 0.11 -1.2 2,922,584 + +0.9 2,922,643 + +0.7 2,922,646 - +0.3 2,922,661 - +0.3 2,922,667 + -2.0 2,922,670 + +0.9 2,922,671 - -1.3 2,922,673 + +0.2 2,922,674 - +0.4 2,922,674 - -1.2 2,922,869 - -0.0 2,922,872 + -2.3 2,922,872 + -1.6 2,922,872 + -0.5 2,922,873 - -0.2 2,922,887 + -1.9 2,922,887 + -0.3 2,922,887 + +1.3 2,922,887 + +0.9 2,922,887 + -0.5 2,922,887 + +1.0 2,922,887 + -0.0 2,922,887 + -2.0 2,922,887 + -0.0 2,922,887 + -2.2 2,922,888 - +1.3 2,922,888 - -1.5 2,922,888 - -1.4 2,922,888 - -1.5 2,922,889 + -2.3 2,922,889 + -1.7 2,922,889 + -0.7 2,922,889 + -1.8 2,922,889 + +0.7 2,922,890 - -0.1 2,922,890 - +0.5 2,922,890 - -0.5 2,922,890 - -1.6 2,922,890 - +0.0 2,922,890 - +0.3 2,922,891 + -2.8 2,922,891 + -2.2 2,922,891 + -1.6 2,922,892 - +0.3 2,922,892 - -0.3 2,922,945 - Psyr_2513 0.13 -1.2 2,922,945 - Psyr_2513 0.13 -1.0 2,923,166 + Psyr_2513 0.34 -0.0 2,923,167 - Psyr_2513 0.34 -0.1 2,923,302 + Psyr_2513 0.46 +0.6 2,923,302 + Psyr_2513 0.46 -1.1 2,923,303 - Psyr_2513 0.47 +0.3 2,923,346 + Psyr_2513 0.51 +0.1 2,923,346 + Psyr_2513 0.51 -0.3 2,923,346 + Psyr_2513 0.51 -0.0 2,923,347 - Psyr_2513 0.51 -1.5 2,923,385 + Psyr_2513 0.54 +0.7 2,923,424 + Psyr_2513 0.58 -1.4 2,923,424 + Psyr_2513 0.58 +0.7 2,923,451 + Psyr_2513 0.60 -1.0 2,923,452 - Psyr_2513 0.60 +1.0 2,923,562 + Psyr_2513 0.71 -0.9 2,923,658 + Psyr_2513 0.79 +0.9 2,923,659 - Psyr_2513 0.80 +0.9 2,923,707 - Psyr_2513 0.84 -1.6 2,923,707 - Psyr_2513 0.84 -0.7 2,923,773 - -0.1 2,923,985 + Psyr_2514 0.14 +1.2 2,924,043 + Psyr_2514 0.22 +0.3 2,924,043 + Psyr_2514 0.22 +0.0 2,924,043 + Psyr_2514 0.22 -1.0 2,924,044 - Psyr_2514 0.22 -0.3 2,924,044 - Psyr_2514 0.22 +1.9 2,924,044 - Psyr_2514 0.22 +0.6 2,924,044 - Psyr_2514 0.22 +0.2 2,924,314 - Psyr_2514 0.60 +0.3 2,924,338 + Psyr_2514 0.63 -0.0 2,924,414 + Psyr_2514 0.74 -0.3 2,924,545 + +0.9 2,924,576 + -1.0 2,924,576 + -0.1 2,924,577 - +0.2 2,924,577 - -0.4 2,924,577 - -0.9 2,924,577 - -0.2 2,924,601 + -0.0 2,924,601 + -0.3 2,924,601 + +1.0 2,924,601 + -0.7 2,924,601 + +0.1 2,924,601 + +0.8 2,924,602 - -0.1 2,924,602 - +0.6 2,924,602 - +1.3 2,924,602 - +1.7 2,924,602 - +0.6 2,924,602 - +1.5 2,924,602 - -1.3
Or see this region's nucleotide sequence