Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2418 and Psyr_2419 are separated by 22 nucleotides Psyr_2419 and Psyr_2420 are separated by 154 nucleotides Psyr_2420 and Psyr_2421 are separated by 283 nucleotides
Psyr_2418: Psyr_2418 - shikimate dehydrogenase, at 2,800,692 to 2,801,507
_2418
Psyr_2419: Psyr_2419 - Protein of unknown function DUF480, at 2,801,530 to 2,802,204
_2419
Psyr_2420: Psyr_2420 - conserved hypothetical protein, at 2,802,359 to 2,802,868
_2420
Psyr_2421: Psyr_2421 - Major facilitator superfamily, at 2,803,152 to 2,804,306
_2421
Position (kb)
2802
2803 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2801.442 kb on + strand at 2801.442 kb on + strand at 2801.443 kb on - strand at 2801.443 kb on - strand at 2801.473 kb on - strand at 2801.532 kb on - strand at 2801.703 kb on - strand, within Psyr_2419 at 2801.703 kb on - strand, within Psyr_2419 at 2801.703 kb on - strand, within Psyr_2419 at 2801.879 kb on - strand, within Psyr_2419 at 2801.879 kb on - strand, within Psyr_2419 at 2802.328 kb on - strand at 2802.328 kb on - strand at 2802.328 kb on - strand at 2802.329 kb on + strand at 2802.329 kb on + strand at 2802.330 kb on - strand at 2802.353 kb on + strand at 2802.353 kb on + strand at 2802.354 kb on - strand at 2802.354 kb on - strand at 2802.354 kb on - strand at 2802.354 kb on - strand at 2802.364 kb on + strand at 2802.364 kb on + strand at 2802.365 kb on - strand at 2802.365 kb on - strand at 2802.365 kb on - strand at 2802.374 kb on + strand at 2802.594 kb on - strand, within Psyr_2420 at 2802.594 kb on - strand, within Psyr_2420 at 2802.755 kb on + strand, within Psyr_2420 at 2802.756 kb on - strand, within Psyr_2420 at 2802.756 kb on - strand, within Psyr_2420 at 2802.756 kb on - strand, within Psyr_2420 at 2802.767 kb on + strand, within Psyr_2420 at 2802.767 kb on + strand, within Psyr_2420 at 2802.807 kb on - strand, within Psyr_2420 at 2802.917 kb on - strand at 2802.917 kb on - strand at 2802.917 kb on - strand at 2802.934 kb on + strand at 2803.087 kb on + strand at 2803.088 kb on - strand at 2803.088 kb on - strand at 2803.147 kb on + strand at 2803.148 kb on - strand at 2803.148 kb on - strand at 2803.422 kb on - strand, within Psyr_2421 at 2803.573 kb on + strand, within Psyr_2421 at 2803.573 kb on + strand, within Psyr_2421 at 2803.681 kb on - strand, within Psyr_2421 at 2803.681 kb on - strand, within Psyr_2421 at 2803.681 kb on - strand, within Psyr_2421 at 2803.809 kb on + strand, within Psyr_2421 at 2803.809 kb on + strand, within Psyr_2421 at 2803.809 kb on + strand, within Psyr_2421 at 2803.809 kb on + strand, within Psyr_2421 at 2803.810 kb on - strand, within Psyr_2421 at 2803.810 kb on - strand, within Psyr_2421 at 2803.811 kb on + strand, within Psyr_2421 at 2803.811 kb on + strand, within Psyr_2421 at 2803.811 kb on + strand, within Psyr_2421 at 2803.811 kb on + strand, within Psyr_2421 at 2803.811 kb on + strand, within Psyr_2421 at 2803.811 kb on + strand, within Psyr_2421 at 2803.811 kb on + strand, within Psyr_2421 at 2803.812 kb on - strand, within Psyr_2421 at 2803.812 kb on - strand, within Psyr_2421 at 2803.812 kb on - strand, within Psyr_2421 at 2803.812 kb on - strand, within Psyr_2421 at 2803.812 kb on - strand, within Psyr_2421 at 2803.812 kb on - strand, within Psyr_2421 at 2803.812 kb on - strand, within Psyr_2421 at 2803.812 kb on - strand, within Psyr_2421 at 2803.815 kb on + strand, within Psyr_2421 at 2803.815 kb on + strand, within Psyr_2421 at 2803.815 kb on + strand, within Psyr_2421 at 2803.816 kb on - strand, within Psyr_2421 at 2803.816 kb on - strand, within Psyr_2421 at 2803.816 kb on - strand, within Psyr_2421
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 2,801,442 + -1.2 2,801,442 + -1.2 2,801,443 - -0.2 2,801,443 - -1.8 2,801,473 - -0.1 2,801,532 - +1.6 2,801,703 - Psyr_2419 0.26 -0.3 2,801,703 - Psyr_2419 0.26 +0.2 2,801,703 - Psyr_2419 0.26 +0.2 2,801,879 - Psyr_2419 0.52 -0.3 2,801,879 - Psyr_2419 0.52 +0.9 2,802,328 - -1.9 2,802,328 - -0.1 2,802,328 - +1.5 2,802,329 + -0.4 2,802,329 + +0.2 2,802,330 - -0.3 2,802,353 + +0.3 2,802,353 + -0.3 2,802,354 - +1.1 2,802,354 - +0.4 2,802,354 - +1.7 2,802,354 - -1.9 2,802,364 + -0.7 2,802,364 + +1.6 2,802,365 - +0.8 2,802,365 - -0.3 2,802,365 - -0.4 2,802,374 + +0.1 2,802,594 - Psyr_2420 0.46 -2.3 2,802,594 - Psyr_2420 0.46 +1.0 2,802,755 + Psyr_2420 0.78 +0.3 2,802,756 - Psyr_2420 0.78 +0.0 2,802,756 - Psyr_2420 0.78 -1.5 2,802,756 - Psyr_2420 0.78 -2.1 2,802,767 + Psyr_2420 0.80 -0.4 2,802,767 + Psyr_2420 0.80 -3.3 2,802,807 - Psyr_2420 0.88 -0.9 2,802,917 - +1.1 2,802,917 - -0.9 2,802,917 - -0.4 2,802,934 + -1.0 2,803,087 + -0.1 2,803,088 - +1.0 2,803,088 - -0.2 2,803,147 + -0.1 2,803,148 - +0.8 2,803,148 - -0.7 2,803,422 - Psyr_2421 0.23 +0.7 2,803,573 + Psyr_2421 0.36 -0.1 2,803,573 + Psyr_2421 0.36 +2.0 2,803,681 - Psyr_2421 0.46 +0.4 2,803,681 - Psyr_2421 0.46 -0.4 2,803,681 - Psyr_2421 0.46 +0.7 2,803,809 + Psyr_2421 0.57 -0.6 2,803,809 + Psyr_2421 0.57 +0.9 2,803,809 + Psyr_2421 0.57 +1.3 2,803,809 + Psyr_2421 0.57 -0.8 2,803,810 - Psyr_2421 0.57 -0.5 2,803,810 - Psyr_2421 0.57 -0.6 2,803,811 + Psyr_2421 0.57 -1.5 2,803,811 + Psyr_2421 0.57 -0.3 2,803,811 + Psyr_2421 0.57 -0.6 2,803,811 + Psyr_2421 0.57 -0.3 2,803,811 + Psyr_2421 0.57 -0.2 2,803,811 + Psyr_2421 0.57 +0.0 2,803,811 + Psyr_2421 0.57 +1.4 2,803,812 - Psyr_2421 0.57 +0.3 2,803,812 - Psyr_2421 0.57 -0.5 2,803,812 - Psyr_2421 0.57 -0.7 2,803,812 - Psyr_2421 0.57 +0.1 2,803,812 - Psyr_2421 0.57 -0.1 2,803,812 - Psyr_2421 0.57 +2.1 2,803,812 - Psyr_2421 0.57 +2.1 2,803,812 - Psyr_2421 0.57 -0.6 2,803,815 + Psyr_2421 0.57 +1.7 2,803,815 + Psyr_2421 0.57 +1.1 2,803,815 + Psyr_2421 0.57 -0.9 2,803,816 - Psyr_2421 0.57 +1.3 2,803,816 - Psyr_2421 0.57 +0.1 2,803,816 - Psyr_2421 0.57 -0.1
Or see this region's nucleotide sequence