Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2249 and Psyr_2250 are separated by 36 nucleotides Psyr_2250 and Psyr_2251 are separated by 14 nucleotides Psyr_2251 and Psyr_2252 overlap by 1 nucleotides Psyr_2252 and Psyr_2253 overlap by 1 nucleotides
Psyr_2249: Psyr_2249 - Binding-protein-dependent transport systems inner membrane component, at 2,615,476 to 2,616,264
_2249
Psyr_2250: Psyr_2250 - transcriptional regulator, GntR family, at 2,616,301 to 2,617,020
_2250
Psyr_2251: Psyr_2251 - Phosphonate metabolism PhnG, at 2,617,035 to 2,617,499
_2251
Psyr_2252: Psyr_2252 - phosphonate metabolism, at 2,617,499 to 2,618,095
_2252
Psyr_2253: Psyr_2253 - Phosphonate metabolism, at 2,618,095 to 2,619,174
_2253
Position (kb)
2617
2618 Strain fitness (log2 ratio)
-2
-1
0
1 at 2616.064 kb on + strand, within Psyr_2249 at 2616.065 kb on - strand, within Psyr_2249 at 2616.065 kb on - strand, within Psyr_2249 at 2616.065 kb on - strand, within Psyr_2249 at 2616.071 kb on + strand, within Psyr_2249 at 2616.215 kb on + strand at 2616.331 kb on + strand at 2616.332 kb on - strand at 2617.299 kb on + strand, within Psyr_2251 at 2617.300 kb on - strand, within Psyr_2251 at 2617.300 kb on - strand, within Psyr_2251 at 2618.098 kb on + strand at 2618.098 kb on + strand at 2618.098 kb on + strand at 2618.099 kb on - strand at 2618.099 kb on - strand at 2618.099 kb on - strand at 2618.099 kb on - strand at 2618.257 kb on + strand, within Psyr_2253 at 2618.464 kb on + strand, within Psyr_2253 at 2618.464 kb on + strand, within Psyr_2253 at 2618.465 kb on - strand, within Psyr_2253
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 2,616,064 + Psyr_2249 0.75 +0.0 2,616,065 - Psyr_2249 0.75 +0.1 2,616,065 - Psyr_2249 0.75 +1.2 2,616,065 - Psyr_2249 0.75 -2.2 2,616,071 + Psyr_2249 0.75 -1.4 2,616,215 + -0.8 2,616,331 + -1.3 2,616,332 - -1.1 2,617,299 + Psyr_2251 0.57 -1.0 2,617,300 - Psyr_2251 0.57 +0.1 2,617,300 - Psyr_2251 0.57 -2.4 2,618,098 + +0.1 2,618,098 + -1.1 2,618,098 + +0.7 2,618,099 - +0.2 2,618,099 - +0.4 2,618,099 - -2.0 2,618,099 - -0.7 2,618,257 + Psyr_2253 0.15 +0.3 2,618,464 + Psyr_2253 0.34 +1.4 2,618,464 + Psyr_2253 0.34 +0.6 2,618,465 - Psyr_2253 0.34 -1.6
Or see this region's nucleotide sequence