Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2191 and Psyr_2192 are separated by 87 nucleotides Psyr_2192 and Psyr_2193 are separated by 77 nucleotides Psyr_2193 and Psyr_2194 overlap by 4 nucleotides
Psyr_2191: Psyr_2191 - RND efflux system, outer membrane lipoprotein, NodT, at 2,546,254 to 2,547,726
_2191
Psyr_2192: Psyr_2192 - transcriptional regulator, TetR family, at 2,547,814 to 2,548,428
_2192
Psyr_2193: Psyr_2193 - Secretion protein HlyD, at 2,548,506 to 2,549,609
_2193
Psyr_2194: Psyr_2194 - Acriflavin resistance protein, at 2,549,606 to 2,552,725
_2194
Position (kb)
2548
2549
2550 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2547.592 kb on - strand at 2547.703 kb on + strand at 2547.703 kb on + strand at 2547.703 kb on + strand at 2547.703 kb on + strand at 2547.704 kb on - strand at 2547.707 kb on + strand at 2547.707 kb on + strand at 2547.707 kb on + strand at 2547.707 kb on + strand at 2547.707 kb on + strand at 2547.707 kb on + strand at 2547.707 kb on + strand at 2547.707 kb on + strand at 2547.708 kb on - strand at 2547.708 kb on - strand at 2547.708 kb on - strand at 2547.708 kb on - strand at 2547.708 kb on - strand at 2547.712 kb on - strand at 2547.712 kb on - strand at 2547.712 kb on - strand at 2547.798 kb on + strand at 2547.799 kb on - strand at 2547.799 kb on - strand at 2547.799 kb on - strand at 2547.799 kb on - strand at 2547.799 kb on - strand at 2547.802 kb on + strand at 2547.802 kb on + strand at 2547.802 kb on + strand at 2547.803 kb on - strand at 2547.803 kb on - strand at 2547.803 kb on - strand at 2547.803 kb on - strand at 2547.970 kb on + strand, within Psyr_2192 at 2547.970 kb on + strand, within Psyr_2192 at 2547.970 kb on + strand, within Psyr_2192 at 2547.970 kb on + strand, within Psyr_2192 at 2547.971 kb on - strand, within Psyr_2192 at 2547.971 kb on - strand, within Psyr_2192 at 2547.971 kb on - strand, within Psyr_2192 at 2547.976 kb on + strand, within Psyr_2192 at 2547.976 kb on + strand, within Psyr_2192 at 2547.976 kb on + strand, within Psyr_2192 at 2547.976 kb on + strand, within Psyr_2192 at 2547.977 kb on - strand, within Psyr_2192 at 2548.159 kb on + strand, within Psyr_2192 at 2548.159 kb on + strand, within Psyr_2192 at 2548.166 kb on + strand, within Psyr_2192 at 2548.334 kb on - strand, within Psyr_2192 at 2548.334 kb on - strand, within Psyr_2192 at 2548.334 kb on - strand, within Psyr_2192 at 2548.334 kb on - strand, within Psyr_2192 at 2548.357 kb on + strand, within Psyr_2192 at 2548.358 kb on - strand, within Psyr_2192 at 2548.358 kb on - strand, within Psyr_2192 at 2548.496 kb on - strand at 2548.543 kb on + strand at 2548.598 kb on + strand at 2549.191 kb on - strand, within Psyr_2193 at 2549.191 kb on - strand, within Psyr_2193 at 2549.262 kb on - strand, within Psyr_2193 at 2549.277 kb on + strand, within Psyr_2193 at 2549.277 kb on + strand at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.278 kb on - strand, within Psyr_2193 at 2549.458 kb on + strand, within Psyr_2193 at 2549.545 kb on + strand at 2549.545 kb on + strand at 2549.545 kb on + strand at 2549.586 kb on - strand at 2549.674 kb on + strand at 2549.761 kb on + strand at 2549.762 kb on - strand at 2549.867 kb on + strand at 2549.867 kb on + strand at 2549.867 kb on + strand at 2549.945 kb on + strand, within Psyr_2194 at 2549.946 kb on - strand, within Psyr_2194
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 2,547,592 - -1.2 2,547,703 + +1.5 2,547,703 + +0.2 2,547,703 + -1.8 2,547,703 + -1.1 2,547,704 - +0.9 2,547,707 + +0.2 2,547,707 + -0.5 2,547,707 + +0.6 2,547,707 + -0.1 2,547,707 + +0.7 2,547,707 + -1.8 2,547,707 + +0.3 2,547,707 + +1.0 2,547,708 - +0.3 2,547,708 - +0.7 2,547,708 - +1.7 2,547,708 - +1.0 2,547,708 - -0.9 2,547,712 - +0.2 2,547,712 - +0.4 2,547,712 - +1.1 2,547,798 + -0.6 2,547,799 - +1.2 2,547,799 - -2.1 2,547,799 - +0.2 2,547,799 - -1.9 2,547,799 - +0.5 2,547,802 + +2.1 2,547,802 + -0.9 2,547,802 + -0.4 2,547,803 - +0.2 2,547,803 - -0.3 2,547,803 - +1.0 2,547,803 - -0.6 2,547,970 + Psyr_2192 0.25 +0.1 2,547,970 + Psyr_2192 0.25 +2.0 2,547,970 + Psyr_2192 0.25 -1.9 2,547,970 + Psyr_2192 0.25 -0.6 2,547,971 - Psyr_2192 0.26 -0.8 2,547,971 - Psyr_2192 0.26 +0.6 2,547,971 - Psyr_2192 0.26 +0.8 2,547,976 + Psyr_2192 0.26 -0.0 2,547,976 + Psyr_2192 0.26 +0.7 2,547,976 + Psyr_2192 0.26 +0.8 2,547,976 + Psyr_2192 0.26 -0.1 2,547,977 - Psyr_2192 0.27 +0.4 2,548,159 + Psyr_2192 0.56 +1.2 2,548,159 + Psyr_2192 0.56 +0.4 2,548,166 + Psyr_2192 0.57 +0.2 2,548,334 - Psyr_2192 0.85 +0.9 2,548,334 - Psyr_2192 0.85 +1.8 2,548,334 - Psyr_2192 0.85 +0.3 2,548,334 - Psyr_2192 0.85 -0.0 2,548,357 + Psyr_2192 0.88 -0.4 2,548,358 - Psyr_2192 0.88 +1.2 2,548,358 - Psyr_2192 0.88 -0.2 2,548,496 - -1.9 2,548,543 + +0.4 2,548,598 + -0.4 2,549,191 - Psyr_2193 0.62 +0.1 2,549,191 - Psyr_2193 0.62 +1.2 2,549,262 - Psyr_2193 0.68 +0.6 2,549,277 + Psyr_2193 0.70 +0.8 2,549,277 + +2.2 2,549,278 - Psyr_2193 0.70 +0.6 2,549,278 - Psyr_2193 0.70 +0.8 2,549,278 - Psyr_2193 0.70 +1.5 2,549,278 - Psyr_2193 0.70 -2.6 2,549,278 - Psyr_2193 0.70 +0.9 2,549,278 - Psyr_2193 0.70 +0.0 2,549,458 + Psyr_2193 0.86 -0.6 2,549,545 + -1.4 2,549,545 + -0.2 2,549,545 + +0.6 2,549,586 - +0.0 2,549,674 + -0.4 2,549,761 + +1.2 2,549,762 - -0.2 2,549,867 + -0.7 2,549,867 + +0.8 2,549,867 + +1.2 2,549,945 + Psyr_2194 0.11 -0.4 2,549,946 - Psyr_2194 0.11 -0.2
Or see this region's nucleotide sequence