Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1945 and Psyr_1946 are separated by 164 nucleotides Psyr_1946 and Psyr_1947 are separated by 81 nucleotides
Psyr_1945: Psyr_1945 - Non-ribosomal peptide synthase:Amino acid adenylation, at 2,217,771 to 2,230,781
_1945
Psyr_1946: Psyr_1946 - diaminobutyrate aminotransferase apoenzyme, at 2,230,946 to 2,232,358
_1946
Psyr_1947: Psyr_1947 - MbtH-like protein, at 2,232,440 to 2,232,658
_1947
Position (kb)
2230
2231
2232
2233 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2230.017 kb on + strand at 2230.018 kb on - strand at 2230.137 kb on + strand at 2230.138 kb on - strand at 2230.138 kb on - strand at 2230.138 kb on - strand at 2230.368 kb on + strand at 2230.369 kb on - strand at 2230.369 kb on - strand at 2230.640 kb on + strand at 2230.904 kb on + strand at 2230.904 kb on + strand at 2230.905 kb on - strand at 2231.015 kb on + strand at 2231.254 kb on + strand, within Psyr_1946 at 2231.255 kb on - strand, within Psyr_1946 at 2231.994 kb on - strand, within Psyr_1946 at 2232.010 kb on + strand, within Psyr_1946 at 2232.261 kb on + strand at 2232.262 kb on - strand at 2232.369 kb on + strand at 2232.369 kb on + strand at 2232.370 kb on - strand at 2232.375 kb on + strand at 2232.375 kb on + strand at 2232.391 kb on - strand at 2232.391 kb on - strand at 2232.391 kb on - strand at 2232.503 kb on + strand, within Psyr_1947 at 2232.504 kb on - strand, within Psyr_1947 at 2232.522 kb on - strand, within Psyr_1947 at 2232.522 kb on - strand, within Psyr_1947 at 2232.587 kb on + strand, within Psyr_1947 at 2232.801 kb on - strand at 2232.817 kb on - strand at 2232.983 kb on - strand at 2233.059 kb on + strand at 2233.060 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 2,230,017 + +0.4 2,230,018 - +1.8 2,230,137 + +0.5 2,230,138 - +0.2 2,230,138 - +0.2 2,230,138 - +0.1 2,230,368 + -2.2 2,230,369 - -1.8 2,230,369 - -1.1 2,230,640 + -0.3 2,230,904 + -0.6 2,230,904 + -0.8 2,230,905 - -0.5 2,231,015 + +0.2 2,231,254 + Psyr_1946 0.22 +0.0 2,231,255 - Psyr_1946 0.22 -0.9 2,231,994 - Psyr_1946 0.74 -1.2 2,232,010 + Psyr_1946 0.75 -1.8 2,232,261 + -0.5 2,232,262 - +1.4 2,232,369 + -0.5 2,232,369 + -1.4 2,232,370 - +0.3 2,232,375 + -0.4 2,232,375 + +0.8 2,232,391 - -0.4 2,232,391 - -1.9 2,232,391 - +0.5 2,232,503 + Psyr_1947 0.29 +0.1 2,232,504 - Psyr_1947 0.29 +0.4 2,232,522 - Psyr_1947 0.37 +0.2 2,232,522 - Psyr_1947 0.37 +0.1 2,232,587 + Psyr_1947 0.67 +1.2 2,232,801 - -1.1 2,232,817 - -0.6 2,232,983 - -1.9 2,233,059 + -0.6 2,233,060 - -0.1
Or see this region's nucleotide sequence