Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1642 and Psyr_1643 are separated by 31 nucleotides Psyr_1643 and Psyr_1644 are separated by 13 nucleotides Psyr_1644 and Psyr_1645 are separated by 34 nucleotides Psyr_1645 and Psyr_1646 are separated by 116 nucleotides
Psyr_1642: Psyr_1642 - Maf-like protein, at 1,849,005 to 1,849,658
_1642
Psyr_1643: Psyr_1643 - Protein of unknown function DUF177, at 1,849,690 to 1,850,217
_1643
Psyr_1644: Psyr_1644 - LSU ribosomal protein L32P, at 1,850,231 to 1,850,413
_1644
Psyr_1645: Psyr_1645 - phosphate:acyl-[acyl carrier protein] acyltransferase, at 1,850,448 to 1,851,440
_1645
Psyr_1646: Psyr_1646 - [Acyl-carrier-protein] S-malonyltransferase, at 1,851,557 to 1,852,495
_1646
Position (kb)
1850
1851
1852 Strain fitness (log2 ratio)
-1
0
1
2 at 1849.449 kb on + strand, within Psyr_1642 at 1849.450 kb on - strand, within Psyr_1642 at 1849.614 kb on - strand at 1850.507 kb on + strand at 1850.508 kb on - strand at 1851.020 kb on - strand, within Psyr_1645 at 1851.039 kb on - strand, within Psyr_1645 at 1851.043 kb on - strand, within Psyr_1645 at 1851.043 kb on - strand, within Psyr_1645 at 1851.043 kb on - strand, within Psyr_1645 at 1851.043 kb on - strand, within Psyr_1645 at 1851.043 kb on - strand, within Psyr_1645 at 1851.043 kb on - strand, within Psyr_1645 at 1851.043 kb on - strand, within Psyr_1645 at 1851.051 kb on + strand, within Psyr_1645 at 1851.052 kb on - strand, within Psyr_1645 at 1851.052 kb on - strand, within Psyr_1645 at 1851.270 kb on - strand, within Psyr_1645 at 1851.487 kb on + strand at 1851.487 kb on + strand at 1851.487 kb on + strand at 1851.487 kb on + strand at 1851.487 kb on + strand at 1851.487 kb on + strand at 1851.487 kb on + strand at 1851.487 kb on + strand at 1851.487 kb on + strand at 1851.487 kb on + strand at 1851.492 kb on + strand at 1851.492 kb on + strand at 1851.538 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 1,849,449 + Psyr_1642 0.68 +0.8 1,849,450 - Psyr_1642 0.68 -1.5 1,849,614 - +0.7 1,850,507 + -1.3 1,850,508 - +0.1 1,851,020 - Psyr_1645 0.58 -0.3 1,851,039 - Psyr_1645 0.60 +0.1 1,851,043 - Psyr_1645 0.60 -0.1 1,851,043 - Psyr_1645 0.60 +0.2 1,851,043 - Psyr_1645 0.60 -0.7 1,851,043 - Psyr_1645 0.60 -1.0 1,851,043 - Psyr_1645 0.60 -0.7 1,851,043 - Psyr_1645 0.60 -0.5 1,851,043 - Psyr_1645 0.60 -0.7 1,851,051 + Psyr_1645 0.61 -0.4 1,851,052 - Psyr_1645 0.61 -1.3 1,851,052 - Psyr_1645 0.61 +0.3 1,851,270 - Psyr_1645 0.83 +0.9 1,851,487 + -1.3 1,851,487 + +1.9 1,851,487 + -0.8 1,851,487 + +0.0 1,851,487 + -0.8 1,851,487 + +0.9 1,851,487 + +0.1 1,851,487 + -1.4 1,851,487 + +0.2 1,851,487 + +0.2 1,851,492 + +0.2 1,851,492 + +1.1 1,851,538 + -0.3
Or see this region's nucleotide sequence