Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1038 and Psyr_1039 are separated by 654 nucleotides Psyr_1039 and Psyr_1040 overlap by 4 nucleotides Psyr_1040 and Psyr_1041 are separated by 210 nucleotides
Psyr_1038: Psyr_1038 - TonB-dependent siderophore receptor, at 1,171,497 to 1,173,845
_1038
Psyr_1039: Psyr_1039 - FecR protein, at 1,174,500 to 1,175,477
_1039
Psyr_1040: Psyr_1040 - RNA polymerase sigma factor, ECF subfamily, at 1,175,474 to 1,175,977
_1040
Psyr_1041: Psyr_1041 - transcriptional regulator, AraC family, at 1,176,188 to 1,177,063
_1041
Position (kb)
1174
1175
1176 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1173.721 kb on + strand at 1173.806 kb on + strand at 1173.806 kb on + strand at 1173.806 kb on + strand at 1173.806 kb on + strand at 1173.806 kb on + strand at 1173.806 kb on + strand at 1173.807 kb on - strand at 1173.807 kb on - strand at 1173.807 kb on - strand at 1173.807 kb on - strand at 1173.807 kb on - strand at 1173.807 kb on - strand at 1173.807 kb on - strand at 1173.955 kb on - strand at 1174.339 kb on + strand at 1174.353 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.458 kb on + strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.459 kb on - strand at 1174.845 kb on + strand, within Psyr_1039 at 1175.203 kb on - strand, within Psyr_1039 at 1175.478 kb on - strand at 1175.487 kb on + strand at 1175.488 kb on - strand at 1175.488 kb on - strand at 1175.488 kb on - strand at 1175.488 kb on - strand at 1175.488 kb on - strand at 1175.721 kb on + strand, within Psyr_1040 at 1175.721 kb on + strand, within Psyr_1040 at 1175.721 kb on + strand, within Psyr_1040 at 1175.722 kb on - strand, within Psyr_1040 at 1175.722 kb on - strand, within Psyr_1040 at 1175.724 kb on + strand, within Psyr_1040 at 1175.724 kb on + strand, within Psyr_1040 at 1175.724 kb on + strand, within Psyr_1040 at 1175.725 kb on - strand, within Psyr_1040 at 1175.819 kb on - strand, within Psyr_1040 at 1175.842 kb on + strand, within Psyr_1040 at 1175.931 kb on + strand at 1175.931 kb on + strand at 1175.931 kb on + strand at 1175.931 kb on + strand at 1176.125 kb on - strand at 1176.189 kb on + strand at 1176.189 kb on + strand at 1176.189 kb on + strand at 1176.189 kb on + strand at 1176.189 kb on + strand at 1176.190 kb on - strand at 1176.190 kb on - strand at 1176.210 kb on + strand at 1176.210 kb on + strand at 1176.211 kb on - strand at 1176.211 kb on - strand at 1176.361 kb on - strand, within Psyr_1041 at 1176.361 kb on - strand, within Psyr_1041
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 1,173,721 + +0.9 1,173,806 + -1.0 1,173,806 + -0.2 1,173,806 + -0.7 1,173,806 + -1.0 1,173,806 + -0.8 1,173,806 + -0.5 1,173,807 - -0.4 1,173,807 - +0.6 1,173,807 - -0.2 1,173,807 - +0.5 1,173,807 - -0.6 1,173,807 - +0.3 1,173,807 - +0.4 1,173,955 - -0.4 1,174,339 + +0.2 1,174,353 + -0.3 1,174,458 + -0.7 1,174,458 + +0.9 1,174,458 + -0.1 1,174,458 + -0.2 1,174,458 + -2.5 1,174,458 + +0.6 1,174,458 + -0.5 1,174,458 + -0.5 1,174,458 + -0.9 1,174,458 + +1.0 1,174,458 + -0.9 1,174,458 + +0.5 1,174,458 + +0.6 1,174,458 + +0.3 1,174,458 + +2.3 1,174,458 + -0.7 1,174,459 - -0.0 1,174,459 - -0.0 1,174,459 - -0.7 1,174,459 - -0.9 1,174,459 - -0.3 1,174,459 - -0.7 1,174,459 - -0.1 1,174,459 - -0.4 1,174,459 - -0.3 1,174,459 - +0.5 1,174,459 - +1.3 1,174,459 - +0.9 1,174,459 - +1.0 1,174,845 + Psyr_1039 0.35 +0.3 1,175,203 - Psyr_1039 0.72 -0.1 1,175,478 - -0.5 1,175,487 + +0.7 1,175,488 - -0.4 1,175,488 - -1.7 1,175,488 - -0.2 1,175,488 - -0.6 1,175,488 - +2.3 1,175,721 + Psyr_1040 0.49 -0.5 1,175,721 + Psyr_1040 0.49 -1.4 1,175,721 + Psyr_1040 0.49 +0.1 1,175,722 - Psyr_1040 0.49 -0.0 1,175,722 - Psyr_1040 0.49 -0.2 1,175,724 + Psyr_1040 0.50 -0.0 1,175,724 + Psyr_1040 0.50 +0.8 1,175,724 + Psyr_1040 0.50 +0.5 1,175,725 - Psyr_1040 0.50 +0.9 1,175,819 - Psyr_1040 0.68 +0.1 1,175,842 + Psyr_1040 0.73 -0.2 1,175,931 + -2.6 1,175,931 + +0.4 1,175,931 + +0.3 1,175,931 + -1.5 1,176,125 - +0.5 1,176,189 + +0.2 1,176,189 + +0.7 1,176,189 + +0.3 1,176,189 + -0.2 1,176,189 + +0.9 1,176,190 - +1.8 1,176,190 - -1.1 1,176,210 + +0.4 1,176,210 + +0.3 1,176,211 - +0.2 1,176,211 - +0.1 1,176,361 - Psyr_1041 0.20 -0.5 1,176,361 - Psyr_1041 0.20 +0.3
Or see this region's nucleotide sequence