Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0567 and Psyr_0568 are separated by 26 nucleotides Psyr_0568 and Psyr_0569 are separated by 14 nucleotides Psyr_0569 and Psyr_0570 overlap by 1 nucleotides
Psyr_0567: Psyr_0567 - Protein of unknown function UPF0031:YjeF-related protein, N-terminal, at 644,229 to 645,719
_0567
Psyr_0568: Psyr_0568 - Protein of unknown function UPF0079, at 645,746 to 646,177
_0568
Psyr_0569: Psyr_0569 - N-acetylmuramoyl-L-alanine amidase, at 646,192 to 647,607
_0569
Psyr_0570: Psyr_0570 - DNA mismatch repair protein MutL, at 647,607 to 649,553
_0570
Position (kb)
646
647
648 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 645.275 kb on + strand, within Psyr_0567 at 645.275 kb on + strand, within Psyr_0567 at 645.275 kb on + strand, within Psyr_0567 at 645.413 kb on + strand, within Psyr_0567 at 645.668 kb on + strand at 645.668 kb on + strand at 645.668 kb on + strand at 645.668 kb on + strand at 645.688 kb on + strand at 646.175 kb on + strand at 646.175 kb on + strand at 646.175 kb on + strand at 646.175 kb on + strand at 646.175 kb on + strand at 646.175 kb on + strand at 646.187 kb on + strand at 646.191 kb on + strand at 647.404 kb on + strand, within Psyr_0569 at 647.404 kb on + strand, within Psyr_0569 at 647.404 kb on + strand, within Psyr_0569 at 647.404 kb on + strand, within Psyr_0569 at 647.404 kb on + strand, within Psyr_0569 at 647.404 kb on + strand, within Psyr_0569 at 647.404 kb on + strand, within Psyr_0569 at 647.404 kb on + strand, within Psyr_0569 at 647.404 kb on + strand, within Psyr_0569 at 647.404 kb on + strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.405 kb on - strand, within Psyr_0569 at 647.500 kb on + strand at 647.500 kb on + strand at 647.500 kb on + strand at 647.501 kb on - strand at 647.501 kb on - strand at 647.501 kb on - strand at 647.578 kb on - strand at 647.894 kb on + strand, within Psyr_0570 at 648.106 kb on + strand, within Psyr_0570 at 648.426 kb on + strand, within Psyr_0570 at 648.426 kb on + strand, within Psyr_0570 at 648.426 kb on + strand, within Psyr_0570 at 648.426 kb on + strand, within Psyr_0570 at 648.427 kb on - strand, within Psyr_0570 at 648.427 kb on - strand, within Psyr_0570
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 645,275 + Psyr_0567 0.70 -1.9 645,275 + Psyr_0567 0.70 -0.2 645,275 + Psyr_0567 0.70 +1.5 645,413 + Psyr_0567 0.79 +0.4 645,668 + +0.3 645,668 + -1.9 645,668 + +1.3 645,668 + -1.6 645,688 + -0.1 646,175 + +0.0 646,175 + -0.5 646,175 + -0.6 646,175 + +1.2 646,175 + +0.3 646,175 + -1.4 646,187 + -0.7 646,191 + -1.6 647,404 + Psyr_0569 0.86 +1.2 647,404 + Psyr_0569 0.86 +1.2 647,404 + Psyr_0569 0.86 -0.9 647,404 + Psyr_0569 0.86 -1.2 647,404 + Psyr_0569 0.86 -1.5 647,404 + Psyr_0569 0.86 +0.5 647,404 + Psyr_0569 0.86 -0.9 647,404 + Psyr_0569 0.86 +0.3 647,404 + Psyr_0569 0.86 -0.1 647,404 + Psyr_0569 0.86 -0.2 647,405 - Psyr_0569 0.86 -0.9 647,405 - Psyr_0569 0.86 -0.5 647,405 - Psyr_0569 0.86 -0.6 647,405 - Psyr_0569 0.86 -0.7 647,405 - Psyr_0569 0.86 -1.2 647,405 - Psyr_0569 0.86 -0.4 647,405 - Psyr_0569 0.86 -0.6 647,405 - Psyr_0569 0.86 -0.6 647,405 - Psyr_0569 0.86 -1.9 647,405 - Psyr_0569 0.86 -0.2 647,405 - Psyr_0569 0.86 -1.1 647,500 + +1.0 647,500 + +0.1 647,500 + +0.1 647,501 - +0.0 647,501 - -1.2 647,501 - -0.6 647,578 - -1.5 647,894 + Psyr_0570 0.15 +0.1 648,106 + Psyr_0570 0.26 -0.2 648,426 + Psyr_0570 0.42 -0.8 648,426 + Psyr_0570 0.42 +0.4 648,426 + Psyr_0570 0.42 +0.7 648,426 + Psyr_0570 0.42 -0.3 648,427 - Psyr_0570 0.42 +0.4 648,427 - Psyr_0570 0.42 +2.0
Or see this region's nucleotide sequence