Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0496 and Psyr_0497 are separated by 15 nucleotides Psyr_0497 and Psyr_0498 are separated by 2 nucleotides Psyr_0498 and Psyr_0499 are separated by 9 nucleotides
Psyr_0496: Psyr_0496 - membrane protein, putative, at 538,075 to 539,307
_0496
Psyr_0497: Psyr_0497 - LamB/YcsF, at 539,323 to 540,087
_0497
Psyr_0498: Psyr_0498 - Protein of unknown function DUF1445, at 540,090 to 540,884
_0498
Psyr_0499: Psyr_0499 - Urea amidolyase-related protein, at 540,894 to 542,498
_0499
Position (kb)
539
540
541 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 538.376 kb on + strand, within Psyr_0496 at 538.376 kb on + strand, within Psyr_0496 at 538.675 kb on + strand, within Psyr_0496 at 538.675 kb on + strand, within Psyr_0496 at 538.675 kb on + strand, within Psyr_0496 at 538.675 kb on + strand, within Psyr_0496 at 538.676 kb on - strand, within Psyr_0496 at 538.676 kb on - strand, within Psyr_0496 at 538.676 kb on - strand, within Psyr_0496 at 538.676 kb on - strand, within Psyr_0496 at 538.677 kb on + strand, within Psyr_0496 at 538.677 kb on + strand, within Psyr_0496 at 538.678 kb on - strand, within Psyr_0496 at 538.684 kb on + strand, within Psyr_0496 at 538.684 kb on + strand, within Psyr_0496 at 538.685 kb on - strand, within Psyr_0496 at 538.685 kb on - strand, within Psyr_0496 at 538.685 kb on - strand, within Psyr_0496 at 538.685 kb on - strand, within Psyr_0496 at 538.685 kb on - strand, within Psyr_0496 at 538.742 kb on + strand, within Psyr_0496 at 538.742 kb on + strand, within Psyr_0496 at 538.742 kb on + strand, within Psyr_0496 at 538.742 kb on + strand, within Psyr_0496 at 538.742 kb on + strand, within Psyr_0496 at 538.742 kb on + strand, within Psyr_0496 at 538.743 kb on - strand, within Psyr_0496 at 538.743 kb on - strand, within Psyr_0496 at 538.743 kb on - strand, within Psyr_0496 at 538.743 kb on - strand, within Psyr_0496 at 538.743 kb on - strand, within Psyr_0496 at 538.743 kb on - strand, within Psyr_0496 at 538.743 kb on - strand, within Psyr_0496 at 538.792 kb on + strand, within Psyr_0496 at 538.793 kb on - strand, within Psyr_0496 at 538.793 kb on - strand, within Psyr_0496 at 538.796 kb on + strand, within Psyr_0496 at 538.796 kb on + strand, within Psyr_0496 at 538.796 kb on + strand, within Psyr_0496 at 538.796 kb on + strand, within Psyr_0496 at 538.796 kb on + strand, within Psyr_0496 at 538.797 kb on - strand, within Psyr_0496 at 538.797 kb on - strand, within Psyr_0496 at 538.797 kb on - strand, within Psyr_0496 at 538.797 kb on - strand, within Psyr_0496 at 538.797 kb on - strand, within Psyr_0496 at 538.797 kb on - strand, within Psyr_0496 at 538.969 kb on + strand, within Psyr_0496 at 538.969 kb on + strand, within Psyr_0496 at 538.969 kb on + strand, within Psyr_0496 at 538.969 kb on + strand, within Psyr_0496 at 538.970 kb on - strand, within Psyr_0496 at 538.970 kb on - strand, within Psyr_0496 at 538.970 kb on - strand, within Psyr_0496 at 538.970 kb on - strand, within Psyr_0496 at 538.970 kb on - strand, within Psyr_0496 at 538.970 kb on - strand, within Psyr_0496 at 538.970 kb on - strand, within Psyr_0496 at 538.970 kb on - strand, within Psyr_0496 at 538.971 kb on + strand, within Psyr_0496 at 538.971 kb on + strand, within Psyr_0496 at 538.971 kb on + strand, within Psyr_0496 at 538.971 kb on + strand, within Psyr_0496 at 538.971 kb on + strand, within Psyr_0496 at 538.971 kb on + strand, within Psyr_0496 at 538.971 kb on + strand, within Psyr_0496 at 538.971 kb on + strand, within Psyr_0496 at 538.971 kb on + strand, within Psyr_0496 at 538.972 kb on - strand, within Psyr_0496 at 538.972 kb on - strand, within Psyr_0496 at 538.972 kb on - strand, within Psyr_0496 at 538.972 kb on - strand, within Psyr_0496 at 539.066 kb on + strand, within Psyr_0496 at 539.066 kb on + strand, within Psyr_0496 at 539.067 kb on - strand, within Psyr_0496 at 539.067 kb on - strand, within Psyr_0496 at 539.068 kb on + strand, within Psyr_0496 at 539.068 kb on + strand, within Psyr_0496 at 539.068 kb on + strand, within Psyr_0496 at 539.069 kb on - strand, within Psyr_0496 at 539.069 kb on - strand, within Psyr_0496 at 539.069 kb on - strand, within Psyr_0496 at 539.069 kb on - strand, within Psyr_0496 at 539.069 kb on - strand, within Psyr_0496 at 539.161 kb on + strand, within Psyr_0496 at 539.161 kb on + strand, within Psyr_0496 at 539.161 kb on + strand, within Psyr_0496 at 539.161 kb on + strand, within Psyr_0496 at 539.161 kb on + strand, within Psyr_0496 at 539.161 kb on + strand, within Psyr_0496 at 539.162 kb on - strand, within Psyr_0496 at 539.162 kb on - strand, within Psyr_0496 at 539.162 kb on - strand, within Psyr_0496 at 539.162 kb on - strand, within Psyr_0496 at 539.162 kb on - strand, within Psyr_0496 at 539.162 kb on - strand, within Psyr_0496 at 539.162 kb on - strand, within Psyr_0496 at 539.222 kb on + strand at 539.254 kb on + strand at 539.254 kb on + strand at 539.254 kb on + strand at 539.254 kb on + strand at 539.254 kb on + strand at 539.255 kb on - strand at 539.255 kb on - strand at 539.255 kb on - strand at 539.255 kb on - strand at 539.280 kb on + strand at 539.281 kb on - strand at 539.333 kb on + strand at 539.333 kb on + strand at 539.333 kb on + strand at 539.333 kb on + strand at 539.334 kb on - strand at 539.334 kb on - strand at 539.334 kb on - strand at 539.334 kb on - strand at 539.590 kb on + strand, within Psyr_0497 at 539.590 kb on + strand, within Psyr_0497 at 539.590 kb on + strand, within Psyr_0497 at 539.591 kb on - strand, within Psyr_0497 at 539.591 kb on - strand, within Psyr_0497 at 539.644 kb on + strand, within Psyr_0497 at 539.645 kb on - strand, within Psyr_0497 at 539.645 kb on - strand, within Psyr_0497 at 539.668 kb on + strand, within Psyr_0497 at 539.668 kb on + strand, within Psyr_0497 at 539.669 kb on - strand, within Psyr_0497 at 539.669 kb on - strand, within Psyr_0497 at 539.834 kb on - strand, within Psyr_0497 at 539.834 kb on - strand, within Psyr_0497 at 540.228 kb on + strand, within Psyr_0498 at 540.228 kb on + strand, within Psyr_0498 at 540.229 kb on - strand, within Psyr_0498 at 540.528 kb on + strand, within Psyr_0498 at 540.528 kb on + strand, within Psyr_0498 at 540.870 kb on + strand at 540.870 kb on + strand at 540.870 kb on + strand at 540.870 kb on + strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.873 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.882 kb on + strand at 540.882 kb on + strand at 540.882 kb on + strand at 540.883 kb on - strand at 540.883 kb on - strand at 540.903 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 538,376 + Psyr_0496 0.24 -1.2 538,376 + Psyr_0496 0.24 +0.2 538,675 + Psyr_0496 0.49 +0.5 538,675 + Psyr_0496 0.49 -0.3 538,675 + Psyr_0496 0.49 +0.2 538,675 + Psyr_0496 0.49 +0.2 538,676 - Psyr_0496 0.49 +0.1 538,676 - Psyr_0496 0.49 -0.0 538,676 - Psyr_0496 0.49 +0.2 538,676 - Psyr_0496 0.49 -0.8 538,677 + Psyr_0496 0.49 -0.9 538,677 + Psyr_0496 0.49 -0.2 538,678 - Psyr_0496 0.49 +1.9 538,684 + Psyr_0496 0.49 +0.1 538,684 + Psyr_0496 0.49 +0.5 538,685 - Psyr_0496 0.49 +0.5 538,685 - Psyr_0496 0.49 +0.7 538,685 - Psyr_0496 0.49 -1.0 538,685 - Psyr_0496 0.49 +0.3 538,685 - Psyr_0496 0.49 +0.1 538,742 + Psyr_0496 0.54 +0.9 538,742 + Psyr_0496 0.54 -1.1 538,742 + Psyr_0496 0.54 +0.1 538,742 + Psyr_0496 0.54 -0.2 538,742 + Psyr_0496 0.54 +0.1 538,742 + Psyr_0496 0.54 +1.2 538,743 - Psyr_0496 0.54 +1.0 538,743 - Psyr_0496 0.54 -0.9 538,743 - Psyr_0496 0.54 -0.6 538,743 - Psyr_0496 0.54 -0.2 538,743 - Psyr_0496 0.54 +0.5 538,743 - Psyr_0496 0.54 +0.3 538,743 - Psyr_0496 0.54 -0.3 538,792 + Psyr_0496 0.58 -0.6 538,793 - Psyr_0496 0.58 -1.8 538,793 - Psyr_0496 0.58 +0.6 538,796 + Psyr_0496 0.58 -0.6 538,796 + Psyr_0496 0.58 -0.1 538,796 + Psyr_0496 0.58 -0.2 538,796 + Psyr_0496 0.58 -0.3 538,796 + Psyr_0496 0.58 +1.5 538,797 - Psyr_0496 0.59 -0.9 538,797 - Psyr_0496 0.59 -0.4 538,797 - Psyr_0496 0.59 +0.5 538,797 - Psyr_0496 0.59 +0.3 538,797 - Psyr_0496 0.59 +0.6 538,797 - Psyr_0496 0.59 +0.2 538,969 + Psyr_0496 0.73 -1.7 538,969 + Psyr_0496 0.73 -1.0 538,969 + Psyr_0496 0.73 +0.5 538,969 + Psyr_0496 0.73 -1.4 538,970 - Psyr_0496 0.73 -0.4 538,970 - Psyr_0496 0.73 -0.1 538,970 - Psyr_0496 0.73 +2.0 538,970 - Psyr_0496 0.73 +0.6 538,970 - Psyr_0496 0.73 -0.5 538,970 - Psyr_0496 0.73 -1.7 538,970 - Psyr_0496 0.73 +1.5 538,970 - Psyr_0496 0.73 -0.7 538,971 + Psyr_0496 0.73 +0.3 538,971 + Psyr_0496 0.73 +0.6 538,971 + Psyr_0496 0.73 +0.4 538,971 + Psyr_0496 0.73 +0.2 538,971 + Psyr_0496 0.73 -0.7 538,971 + Psyr_0496 0.73 -1.0 538,971 + Psyr_0496 0.73 +0.1 538,971 + Psyr_0496 0.73 +0.9 538,971 + Psyr_0496 0.73 -0.5 538,972 - Psyr_0496 0.73 +0.8 538,972 - Psyr_0496 0.73 +0.3 538,972 - Psyr_0496 0.73 -0.1 538,972 - Psyr_0496 0.73 +3.5 539,066 + Psyr_0496 0.80 -0.6 539,066 + Psyr_0496 0.80 +0.2 539,067 - Psyr_0496 0.80 -2.2 539,067 - Psyr_0496 0.80 +1.3 539,068 + Psyr_0496 0.81 -1.5 539,068 + Psyr_0496 0.81 +0.6 539,068 + Psyr_0496 0.81 +0.4 539,069 - Psyr_0496 0.81 -1.2 539,069 - Psyr_0496 0.81 +0.5 539,069 - Psyr_0496 0.81 -0.3 539,069 - Psyr_0496 0.81 -1.7 539,069 - Psyr_0496 0.81 -0.3 539,161 + Psyr_0496 0.88 +0.1 539,161 + Psyr_0496 0.88 -0.6 539,161 + Psyr_0496 0.88 -0.0 539,161 + Psyr_0496 0.88 -0.1 539,161 + Psyr_0496 0.88 -0.3 539,161 + Psyr_0496 0.88 -0.4 539,162 - Psyr_0496 0.88 -0.2 539,162 - Psyr_0496 0.88 -0.3 539,162 - Psyr_0496 0.88 +1.0 539,162 - Psyr_0496 0.88 -0.5 539,162 - Psyr_0496 0.88 +0.3 539,162 - Psyr_0496 0.88 -0.7 539,162 - Psyr_0496 0.88 +0.0 539,222 + +0.7 539,254 + +0.8 539,254 + -0.7 539,254 + +1.9 539,254 + -1.5 539,254 + +0.6 539,255 - -0.5 539,255 - -2.1 539,255 - +0.2 539,255 - -0.2 539,280 + -0.0 539,281 - -0.1 539,333 + -0.5 539,333 + +1.2 539,333 + +0.6 539,333 + +0.6 539,334 - -1.3 539,334 - +0.1 539,334 - +0.7 539,334 - +0.6 539,590 + Psyr_0497 0.35 +0.0 539,590 + Psyr_0497 0.35 -0.1 539,590 + Psyr_0497 0.35 -0.5 539,591 - Psyr_0497 0.35 -1.1 539,591 - Psyr_0497 0.35 -0.3 539,644 + Psyr_0497 0.42 +0.5 539,645 - Psyr_0497 0.42 -0.5 539,645 - Psyr_0497 0.42 -0.3 539,668 + Psyr_0497 0.45 -1.7 539,668 + Psyr_0497 0.45 -0.4 539,669 - Psyr_0497 0.45 -0.2 539,669 - Psyr_0497 0.45 -0.4 539,834 - Psyr_0497 0.67 -0.7 539,834 - Psyr_0497 0.67 +0.0 540,228 + Psyr_0498 0.17 +0.2 540,228 + Psyr_0498 0.17 +0.5 540,229 - Psyr_0498 0.17 -0.0 540,528 + Psyr_0498 0.55 -0.4 540,528 + Psyr_0498 0.55 +0.1 540,870 + +0.3 540,870 + +0.1 540,870 + +0.2 540,870 + -1.9 540,871 - +0.8 540,871 - -1.5 540,871 - -0.1 540,871 - -1.0 540,871 - +0.3 540,871 - +0.7 540,871 - -0.0 540,871 - -0.4 540,871 - -0.9 540,871 - +0.1 540,873 + +0.3 540,880 + +0.4 540,880 + -0.9 540,880 + -0.6 540,880 + +1.5 540,880 + -0.4 540,880 + +0.2 540,880 + -0.3 540,880 + +0.1 540,880 + -0.5 540,880 + -1.9 540,881 - +0.8 540,881 - -0.8 540,881 - +0.5 540,881 - -1.5 540,881 - +1.5 540,881 - +1.3 540,881 - +0.5 540,881 - +0.6 540,881 - -0.3 540,882 + +0.6 540,882 + -0.6 540,882 + -0.6 540,883 - +0.3 540,883 - -1.0 540,903 + +0.5
Or see this region's nucleotide sequence