Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0456 and Psyr_0457 are separated by 27 nucleotides Psyr_0457 and Psyr_0458 are separated by 393 nucleotides Psyr_0458 and Psyr_0459 are separated by 311 nucleotides
Psyr_0456: Psyr_0456 - Cytochrome B561, bacterial, at 493,396 to 493,944
_0456
Psyr_0457: Psyr_0457 - YceI, at 493,972 to 494,550
_0457
Psyr_0458: Psyr_0458 - Helicase, C-terminal:Type III restriction enzyme, res subunit:DEAD/DEAH box helicase, N-terminal, at 494,944 to 496,833
_0458
Psyr_0459: Psyr_0459 - 5,10-methylenetetrahydrofolate reductase, at 497,145 to 497,990
_0459
Position (kb) 494
495
496
497 Strain fitness (log2 ratio) -2
-1
0
1
2 at 493.965 kb on + strand at 494.047 kb on + strand, within Psyr_0457 at 494.047 kb on + strand, within Psyr_0457 at 494.047 kb on + strand, within Psyr_0457 at 494.047 kb on + strand, within Psyr_0457 at 494.048 kb on - strand, within Psyr_0457 at 494.108 kb on - strand, within Psyr_0457 at 494.108 kb on - strand, within Psyr_0457 at 494.124 kb on + strand, within Psyr_0457 at 494.124 kb on + strand, within Psyr_0457 at 494.125 kb on - strand, within Psyr_0457 at 494.278 kb on + strand, within Psyr_0457 at 494.278 kb on + strand, within Psyr_0457 at 494.278 kb on + strand, within Psyr_0457 at 494.366 kb on + strand, within Psyr_0457 at 494.537 kb on + strand at 494.538 kb on - strand at 494.538 kb on - strand at 494.548 kb on + strand at 494.548 kb on + strand at 494.548 kb on + strand at 494.548 kb on + strand at 494.548 kb on + strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.665 kb on + strand at 494.665 kb on + strand at 494.666 kb on - strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.753 kb on - strand at 494.753 kb on - strand at 494.753 kb on - strand at 494.753 kb on - strand at 494.753 kb on - strand at 494.754 kb on + strand at 494.808 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.843 kb on + strand at 494.843 kb on + strand at 494.843 kb on + strand at 494.844 kb on - strand at 494.844 kb on - strand at 494.844 kb on - strand at 494.889 kb on - strand at 494.889 kb on - strand at 495.362 kb on + strand, within Psyr_0458 at 495.362 kb on + strand, within Psyr_0458 at 495.363 kb on - strand, within Psyr_0458 at 495.388 kb on + strand, within Psyr_0458 at 495.388 kb on + strand, within Psyr_0458 at 495.389 kb on - strand, within Psyr_0458 at 495.389 kb on - strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 496.258 kb on - strand, within Psyr_0458 at 496.683 kb on + strand at 496.684 kb on - strand at 496.837 kb on + strand at 496.837 kb on + strand at 496.837 kb on + strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.897 kb on - strand at 497.042 kb on + strand at 497.042 kb on + strand at 497.043 kb on - strand at 497.043 kb on - strand at 497.043 kb on - strand at 497.044 kb on + strand at 497.044 kb on + strand at 497.044 kb on + strand at 497.045 kb on - strand at 497.045 kb on - strand at 497.045 kb on - strand at 497.061 kb on + strand at 497.089 kb on + strand at 497.089 kb on + strand at 497.090 kb on - strand at 497.090 kb on - strand at 497.090 kb on - strand at 497.207 kb on - strand at 497.207 kb on - strand at 497.306 kb on - strand, within Psyr_0459 at 497.322 kb on - strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.729 kb on - strand, within Psyr_0459 at 497.729 kb on - strand, within Psyr_0459 at 497.769 kb on + strand, within Psyr_0459
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 493,965 + -2.0 494,047 + Psyr_0457 0.13 +0.3 494,047 + Psyr_0457 0.13 -0.0 494,047 + Psyr_0457 0.13 -0.1 494,047 + Psyr_0457 0.13 -1.2 494,048 - Psyr_0457 0.13 -1.3 494,108 - Psyr_0457 0.23 -1.1 494,108 - Psyr_0457 0.23 -0.5 494,124 + Psyr_0457 0.26 +0.3 494,124 + Psyr_0457 0.26 +0.5 494,125 - Psyr_0457 0.26 -0.5 494,278 + Psyr_0457 0.53 +0.2 494,278 + Psyr_0457 0.53 +0.6 494,278 + Psyr_0457 0.53 +1.3 494,366 + Psyr_0457 0.68 -0.1 494,537 + +0.2 494,538 - +0.5 494,538 - +1.7 494,548 + -0.8 494,548 + -0.2 494,548 + +0.2 494,548 + -0.1 494,548 + +0.4 494,549 - -1.2 494,549 - -0.8 494,549 - +0.8 494,549 - +0.2 494,549 - +1.0 494,549 - +0.8 494,665 + -0.6 494,665 + +0.9 494,666 - +0.5 494,667 + -0.0 494,667 + -0.3 494,667 + +0.5 494,667 + -0.0 494,667 + +0.1 494,667 + -0.9 494,667 + +0.7 494,667 + +0.6 494,667 + -0.7 494,667 + +0.7 494,667 + +0.3 494,667 + +0.0 494,667 + +0.9 494,667 + +0.6 494,667 + -0.7 494,667 + -0.2 494,667 + -0.0 494,667 + -1.1 494,667 + -0.6 494,667 + -0.3 494,667 + -1.2 494,667 + +0.9 494,667 + -0.4 494,668 - -0.8 494,668 - -0.1 494,668 - +1.0 494,668 - +1.1 494,668 - +0.5 494,668 - -0.2 494,668 - +1.3 494,668 - +0.6 494,668 - -0.1 494,668 - -0.2 494,668 - +0.2 494,668 - -0.7 494,668 - +0.4 494,668 - -0.5 494,668 - -1.7 494,668 - +1.2 494,668 - +0.1 494,752 + +0.1 494,752 + +0.3 494,752 + -0.5 494,752 + +0.7 494,752 + -2.0 494,752 + -0.1 494,752 + +1.5 494,753 - -0.1 494,753 - +0.2 494,753 - +0.2 494,753 - +0.8 494,753 - +0.2 494,754 + +1.1 494,808 + -0.2 494,839 + +1.0 494,839 + -0.7 494,839 + +0.6 494,839 + +0.5 494,839 + -0.5 494,839 + -0.2 494,840 - -0.1 494,840 - -1.6 494,840 - -1.3 494,840 - +1.0 494,840 - -0.4 494,840 - -0.3 494,840 - +1.3 494,843 + +1.5 494,843 + +0.0 494,843 + -0.7 494,844 - +0.7 494,844 - +0.1 494,844 - +0.5 494,889 - -0.1 494,889 - +0.9 495,362 + Psyr_0458 0.22 +0.1 495,362 + Psyr_0458 0.22 -0.1 495,363 - Psyr_0458 0.22 -0.9 495,388 + Psyr_0458 0.23 -0.9 495,388 + Psyr_0458 0.23 -0.9 495,389 - Psyr_0458 0.24 +0.8 495,389 - Psyr_0458 0.24 +0.2 495,836 + Psyr_0458 0.47 +0.1 495,836 + Psyr_0458 0.47 -0.9 495,836 + Psyr_0458 0.47 +0.1 495,836 + Psyr_0458 0.47 -0.4 495,836 + Psyr_0458 0.47 -0.3 495,837 - Psyr_0458 0.47 +0.8 495,837 - Psyr_0458 0.47 +0.4 495,837 - Psyr_0458 0.47 -0.7 495,837 - Psyr_0458 0.47 -0.9 495,837 - Psyr_0458 0.47 -0.7 495,837 - Psyr_0458 0.47 +0.7 495,934 + Psyr_0458 0.52 +0.9 495,934 + Psyr_0458 0.52 +0.2 495,934 + Psyr_0458 0.52 +0.6 495,934 + Psyr_0458 0.52 -1.1 495,934 + Psyr_0458 0.52 -0.3 495,934 + Psyr_0458 0.52 +0.2 495,934 + Psyr_0458 0.52 +0.1 495,934 + Psyr_0458 0.52 -0.5 495,935 - Psyr_0458 0.52 -1.4 495,935 - Psyr_0458 0.52 -0.3 495,935 - Psyr_0458 0.52 +0.3 495,935 - Psyr_0458 0.52 +0.7 495,935 - Psyr_0458 0.52 -0.8 495,935 - Psyr_0458 0.52 +0.1 495,935 - Psyr_0458 0.52 +0.4 495,935 - Psyr_0458 0.52 -0.1 495,935 - Psyr_0458 0.52 +1.1 496,258 - Psyr_0458 0.70 -0.8 496,683 + +0.2 496,684 - +1.2 496,837 + -0.1 496,837 + -1.2 496,837 + +0.8 496,858 + -1.1 496,858 + -0.3 496,858 + -0.5 496,858 + +0.1 496,897 - -1.2 497,042 + -2.1 497,042 + -0.1 497,043 - +1.5 497,043 - +0.9 497,043 - +0.1 497,044 + -0.8 497,044 + +0.0 497,044 + -0.1 497,045 - -0.3 497,045 - -1.1 497,045 - -0.5 497,061 + -0.5 497,089 + -0.1 497,089 + -0.4 497,090 - +0.3 497,090 - +1.2 497,090 - -0.9 497,207 - +1.1 497,207 - +0.2 497,306 - Psyr_0459 0.19 -1.1 497,322 - Psyr_0459 0.21 -0.3 497,380 + Psyr_0459 0.28 -0.9 497,380 + Psyr_0459 0.28 -2.3 497,380 + Psyr_0459 0.28 -0.0 497,380 + Psyr_0459 0.28 +1.0 497,380 + Psyr_0459 0.28 +0.1 497,381 - Psyr_0459 0.28 +0.6 497,381 - Psyr_0459 0.28 +0.2 497,381 - Psyr_0459 0.28 -1.7 497,381 - Psyr_0459 0.28 +0.7 497,381 - Psyr_0459 0.28 +1.1 497,458 + Psyr_0459 0.37 -0.0 497,458 + Psyr_0459 0.37 -0.3 497,458 + Psyr_0459 0.37 +0.2 497,458 + Psyr_0459 0.37 -1.1 497,458 + Psyr_0459 0.37 +0.1 497,458 + Psyr_0459 0.37 +2.5 497,458 + Psyr_0459 0.37 +0.7 497,458 + Psyr_0459 0.37 +0.5 497,458 + Psyr_0459 0.37 -0.5 497,459 - Psyr_0459 0.37 +0.7 497,459 - Psyr_0459 0.37 -0.4 497,459 - Psyr_0459 0.37 -0.2 497,479 + Psyr_0459 0.39 +0.4 497,479 + Psyr_0459 0.39 +0.1 497,479 + Psyr_0459 0.39 -1.1 497,480 - Psyr_0459 0.40 +0.5 497,480 - Psyr_0459 0.40 +0.3 497,728 + Psyr_0459 0.69 -0.3 497,729 - Psyr_0459 0.69 -0.9 497,729 - Psyr_0459 0.69 +0.4 497,769 + Psyr_0459 0.74 +1.2
Or see this region's nucleotide sequence