Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0441 and Psyr_0442 are separated by 406 nucleotides Psyr_0442 and Psyr_0443 overlap by 1 nucleotides Psyr_0443 and Psyr_0444 are separated by 1 nucleotides
Psyr_0441: Psyr_0441 - conserved hypothetical protein, at 478,811 to 479,206
_0441
Psyr_0442: Psyr_0442 - Malonate decarboxylase, alpha subunit, at 479,613 to 481,289
_0442
Psyr_0443: Psyr_0443 - Triphosphoribosyl-dephospho-CoA synthase, at 481,289 to 482,164
_0443
Psyr_0444: Psyr_0444 - Malonate decarboxylase delta subunit, at 482,166 to 482,465
_0444
Position (kb)
479
480
481
482 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.812 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.969 kb on + strand, within Psyr_0441 at 478.970 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.156 kb on + strand, within Psyr_0441 at 479.196 kb on - strand at 479.196 kb on - strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.255 kb on - strand at 479.283 kb on + strand at 479.296 kb on + strand at 479.305 kb on - strand at 479.370 kb on - strand at 479.376 kb on - strand at 479.464 kb on - strand at 479.577 kb on + strand at 479.578 kb on - strand at 479.578 kb on - strand at 479.686 kb on + strand at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.024 kb on + strand, within Psyr_0442 at 480.025 kb on - strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.274 kb on - strand, within Psyr_0442 at 480.274 kb on - strand, within Psyr_0442 at 480.274 kb on - strand, within Psyr_0442 at 480.274 kb on - strand, within Psyr_0442 at 480.357 kb on + strand, within Psyr_0442 at 480.358 kb on - strand, within Psyr_0442 at 480.358 kb on - strand, within Psyr_0442 at 480.358 kb on - strand, within Psyr_0442 at 480.358 kb on - strand, within Psyr_0442 at 480.436 kb on - strand, within Psyr_0442 at 480.436 kb on - strand, within Psyr_0442 at 480.573 kb on + strand, within Psyr_0442 at 480.574 kb on - strand, within Psyr_0442 at 480.574 kb on - strand, within Psyr_0442 at 480.574 kb on - strand, within Psyr_0442 at 480.849 kb on + strand, within Psyr_0442 at 480.987 kb on + strand, within Psyr_0442 at 480.987 kb on + strand, within Psyr_0442 at 480.988 kb on - strand, within Psyr_0442 at 481.032 kb on + strand, within Psyr_0442 at 481.038 kb on + strand, within Psyr_0442 at 481.038 kb on + strand, within Psyr_0442 at 481.039 kb on - strand, within Psyr_0442 at 481.039 kb on - strand, within Psyr_0442 at 481.258 kb on - strand at 481.258 kb on - strand at 481.258 kb on - strand at 481.258 kb on - strand at 481.258 kb on - strand at 481.600 kb on + strand, within Psyr_0443 at 481.600 kb on + strand, within Psyr_0443 at 481.601 kb on - strand, within Psyr_0443 at 481.601 kb on - strand, within Psyr_0443 at 481.601 kb on - strand, within Psyr_0443 at 481.603 kb on + strand, within Psyr_0443 at 481.604 kb on - strand, within Psyr_0443 at 481.604 kb on - strand, within Psyr_0443 at 481.853 kb on - strand, within Psyr_0443 at 481.853 kb on - strand, within Psyr_0443 at 481.916 kb on - strand, within Psyr_0443 at 481.998 kb on - strand, within Psyr_0443
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 478,743 + +1.2 478,743 + -0.5 478,743 + -1.0 478,743 + +1.5 478,743 + -0.3 478,812 + -0.4 478,829 + -0.8 478,829 + -0.2 478,829 + -1.7 478,829 + -0.6 478,830 - +0.5 478,830 - +0.3 478,830 - -0.0 478,830 - +0.9 478,830 - +0.4 478,830 - +0.1 478,830 - -0.5 478,830 - -0.3 478,830 - -1.2 478,830 - +0.5 478,969 + Psyr_0441 0.40 +0.9 478,970 - Psyr_0441 0.40 -0.5 479,041 - Psyr_0441 0.58 +1.0 479,041 - Psyr_0441 0.58 +0.5 479,041 - Psyr_0441 0.58 +2.1 479,156 + Psyr_0441 0.87 +1.8 479,196 - -0.4 479,196 - +1.3 479,197 + +1.1 479,197 + +0.0 479,197 + +0.5 479,197 + -0.6 479,197 + -0.3 479,198 - +1.6 479,198 - +0.4 479,198 - -0.5 479,198 - +0.1 479,198 - +2.0 479,198 - +1.7 479,198 - -1.2 479,198 - -0.5 479,198 - -0.0 479,198 - +0.1 479,198 - +0.3 479,198 - +0.2 479,255 - -0.2 479,283 + +0.3 479,296 + +0.8 479,305 - -0.3 479,370 - -0.7 479,376 - +1.5 479,464 - +3.3 479,577 + -0.8 479,578 - +0.5 479,578 - +1.3 479,686 + -0.4 480,012 + Psyr_0442 0.24 +0.0 480,012 + Psyr_0442 0.24 -0.8 480,012 + Psyr_0442 0.24 +0.6 480,012 + Psyr_0442 0.24 +0.0 480,012 + Psyr_0442 0.24 +0.0 480,012 + Psyr_0442 0.24 +1.5 480,013 - Psyr_0442 0.24 -1.2 480,013 - Psyr_0442 0.24 -0.5 480,013 - Psyr_0442 0.24 -0.1 480,013 - Psyr_0442 0.24 -0.7 480,013 - Psyr_0442 0.24 -2.0 480,024 + Psyr_0442 0.25 +1.1 480,025 - Psyr_0442 0.25 +0.4 480,102 + Psyr_0442 0.29 +0.1 480,102 + Psyr_0442 0.29 -0.3 480,102 + Psyr_0442 0.29 +0.4 480,102 + Psyr_0442 0.29 -0.6 480,103 - Psyr_0442 0.29 +2.8 480,103 - Psyr_0442 0.29 +0.5 480,103 - Psyr_0442 0.29 +0.3 480,103 - Psyr_0442 0.29 +0.6 480,103 - Psyr_0442 0.29 +1.7 480,103 - Psyr_0442 0.29 +0.9 480,273 + Psyr_0442 0.39 -0.3 480,273 + Psyr_0442 0.39 -0.9 480,273 + Psyr_0442 0.39 -0.1 480,273 + Psyr_0442 0.39 -0.2 480,273 + Psyr_0442 0.39 +2.5 480,273 + Psyr_0442 0.39 +1.3 480,273 + Psyr_0442 0.39 +0.4 480,273 + Psyr_0442 0.39 -1.3 480,273 + Psyr_0442 0.39 -0.2 480,273 + Psyr_0442 0.39 +1.8 480,274 - Psyr_0442 0.39 +0.9 480,274 - Psyr_0442 0.39 +0.5 480,274 - Psyr_0442 0.39 +1.5 480,274 - Psyr_0442 0.39 +0.1 480,357 + Psyr_0442 0.44 +0.9 480,358 - Psyr_0442 0.44 +0.5 480,358 - Psyr_0442 0.44 -1.6 480,358 - Psyr_0442 0.44 +0.2 480,358 - Psyr_0442 0.44 +1.0 480,436 - Psyr_0442 0.49 +0.1 480,436 - Psyr_0442 0.49 -0.5 480,573 + Psyr_0442 0.57 +0.0 480,574 - Psyr_0442 0.57 -1.6 480,574 - Psyr_0442 0.57 -0.3 480,574 - Psyr_0442 0.57 -0.2 480,849 + Psyr_0442 0.74 -0.5 480,987 + Psyr_0442 0.82 -0.0 480,987 + Psyr_0442 0.82 -1.1 480,988 - Psyr_0442 0.82 +0.8 481,032 + Psyr_0442 0.85 +0.2 481,038 + Psyr_0442 0.85 +0.1 481,038 + Psyr_0442 0.85 +0.8 481,039 - Psyr_0442 0.85 +0.2 481,039 - Psyr_0442 0.85 +1.0 481,258 - -0.8 481,258 - -2.3 481,258 - +0.8 481,258 - -0.7 481,258 - -0.3 481,600 + Psyr_0443 0.36 +0.0 481,600 + Psyr_0443 0.36 -0.1 481,601 - Psyr_0443 0.36 -1.7 481,601 - Psyr_0443 0.36 +0.6 481,601 - Psyr_0443 0.36 +1.0 481,603 + Psyr_0443 0.36 -0.5 481,604 - Psyr_0443 0.36 -0.0 481,604 - Psyr_0443 0.36 -1.3 481,853 - Psyr_0443 0.64 +1.1 481,853 - Psyr_0443 0.64 +1.3 481,916 - Psyr_0443 0.72 +0.0 481,998 - Psyr_0443 0.81 -0.0
Or see this region's nucleotide sequence