Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0263 and Psyr_0264 are separated by 10 nucleotides Psyr_0264 and Psyr_0265 are separated by 53 nucleotides Psyr_0265 and Psyr_0266 are separated by 23 nucleotides
Psyr_0263: Psyr_0263 - Two-component response regulator AlgB, at 278,848 to 280,194
_0263
Psyr_0264: Psyr_0264 - Alginate biosynthesis sensor protein KinB, at 280,205 to 281,992
_0264
Psyr_0265: Psyr_0265 - N-acetylmuramoyl-L-alanine amidase, family 2, at 282,046 to 282,897
_0265
Psyr_0266: Psyr_0266 - GGDEF domain protein, at 282,921 to 284,993
_0266
Position (kb) 280
281
282 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 279.650 kb on - strand, within Psyr_0263 at 279.763 kb on + strand, within Psyr_0263 at 279.764 kb on - strand, within Psyr_0263 at 279.865 kb on + strand, within Psyr_0263 at 279.865 kb on + strand, within Psyr_0263 at 279.865 kb on + strand, within Psyr_0263 at 279.866 kb on - strand, within Psyr_0263 at 279.866 kb on - strand, within Psyr_0263 at 279.919 kb on + strand, within Psyr_0263 at 279.995 kb on + strand, within Psyr_0263 at 279.995 kb on + strand, within Psyr_0263 at 279.996 kb on - strand, within Psyr_0263 at 280.100 kb on + strand at 280.100 kb on + strand at 280.101 kb on - strand at 280.183 kb on + strand at 280.183 kb on + strand at 280.183 kb on + strand at 280.184 kb on - strand at 280.184 kb on - strand at 280.184 kb on - strand at 280.190 kb on + strand at 280.190 kb on + strand at 280.190 kb on + strand at 280.190 kb on + strand at 280.191 kb on - strand at 280.191 kb on - strand at 280.197 kb on + strand at 280.197 kb on + strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.319 kb on - strand at 280.342 kb on + strand at 280.342 kb on + strand at 280.342 kb on + strand at 280.342 kb on + strand at 280.538 kb on + strand, within Psyr_0264 at 280.539 kb on - strand, within Psyr_0264 at 280.568 kb on + strand, within Psyr_0264 at 280.569 kb on - strand, within Psyr_0264 at 280.590 kb on + strand, within Psyr_0264 at 280.590 kb on + strand, within Psyr_0264 at 280.591 kb on - strand, within Psyr_0264 at 280.591 kb on - strand, within Psyr_0264 at 280.591 kb on - strand, within Psyr_0264 at 281.003 kb on - strand, within Psyr_0264 at 281.003 kb on - strand, within Psyr_0264 at 281.234 kb on + strand, within Psyr_0264 at 281.235 kb on - strand, within Psyr_0264 at 281.623 kb on + strand, within Psyr_0264 at 281.642 kb on - strand, within Psyr_0264 at 281.942 kb on + strand at 281.943 kb on - strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.974 kb on + strand at 281.975 kb on - strand at 282.192 kb on - strand, within Psyr_0265 at 282.369 kb on + strand, within Psyr_0265 at 282.370 kb on - strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.638 kb on + strand, within Psyr_0265 at 282.638 kb on + strand, within Psyr_0265 at 282.639 kb on - strand, within Psyr_0265 at 282.667 kb on + strand, within Psyr_0265 at 282.668 kb on - strand, within Psyr_0265 at 282.668 kb on - strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.716 kb on + strand, within Psyr_0265 at 282.716 kb on + strand, within Psyr_0265 at 282.716 kb on + strand, within Psyr_0265 at 282.716 kb on + strand, within Psyr_0265 at 282.716 kb on + strand, within Psyr_0265 at 282.717 kb on - strand, within Psyr_0265 at 282.717 kb on - strand, within Psyr_0265 at 282.717 kb on - strand, within Psyr_0265 at 282.811 kb on + strand, within Psyr_0265 at 282.811 kb on + strand, within Psyr_0265 at 282.815 kb on + strand at 282.815 kb on + strand at 282.815 kb on + strand at 282.815 kb on + strand at 282.816 kb on - strand at 282.816 kb on - strand at 282.816 kb on - strand at 282.816 kb on - strand at 282.870 kb on + strand at 282.872 kb on + strand at 282.873 kb on - strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.964 kb on + strand at 282.964 kb on + strand at 282.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 279,650 - Psyr_0263 0.60 -0.5 279,763 + Psyr_0263 0.68 -1.2 279,764 - Psyr_0263 0.68 -1.8 279,865 + Psyr_0263 0.76 +0.9 279,865 + Psyr_0263 0.76 +0.4 279,865 + Psyr_0263 0.76 -1.8 279,866 - Psyr_0263 0.76 -0.1 279,866 - Psyr_0263 0.76 +0.4 279,919 + Psyr_0263 0.80 -0.4 279,995 + Psyr_0263 0.85 -0.1 279,995 + Psyr_0263 0.85 +0.2 279,996 - Psyr_0263 0.85 +0.3 280,100 + +1.3 280,100 + -0.1 280,101 - +0.1 280,183 + -1.1 280,183 + +0.2 280,183 + -0.2 280,184 - -0.1 280,184 - +0.8 280,184 - -2.9 280,190 + +0.9 280,190 + -0.8 280,190 + +0.7 280,190 + -0.3 280,191 - +0.3 280,191 - +0.9 280,197 + +0.4 280,197 + -0.5 280,198 - +1.5 280,198 - +0.0 280,198 - -0.1 280,198 - +1.2 280,198 - +0.3 280,319 - -1.3 280,342 + -0.9 280,342 + -0.6 280,342 + -1.2 280,342 + -0.6 280,538 + Psyr_0264 0.19 +1.8 280,539 - Psyr_0264 0.19 +0.4 280,568 + Psyr_0264 0.20 +0.0 280,569 - Psyr_0264 0.20 +0.4 280,590 + Psyr_0264 0.22 +0.1 280,590 + Psyr_0264 0.22 -0.6 280,591 - Psyr_0264 0.22 +0.5 280,591 - Psyr_0264 0.22 -0.6 280,591 - Psyr_0264 0.22 +2.1 281,003 - Psyr_0264 0.45 +2.1 281,003 - Psyr_0264 0.45 -0.4 281,234 + Psyr_0264 0.58 -0.6 281,235 - Psyr_0264 0.58 -0.6 281,623 + Psyr_0264 0.79 +0.7 281,642 - Psyr_0264 0.80 +0.7 281,942 + +0.2 281,943 - -0.3 281,972 + -0.1 281,972 + -0.5 281,972 + +1.8 281,972 + +0.3 281,972 + -1.2 281,972 + +1.5 281,972 + -0.1 281,972 + +0.4 281,972 + +1.0 281,972 + -0.7 281,972 + -0.6 281,972 + +0.9 281,972 + +1.5 281,972 + +0.4 281,972 + -1.6 281,972 + -0.7 281,972 + +0.1 281,972 + +0.7 281,972 + +0.6 281,972 + +0.9 281,973 - -1.2 281,973 - +0.0 281,973 - -0.6 281,973 - +0.5 281,973 - +0.8 281,973 - -1.2 281,973 - +0.0 281,973 - +1.1 281,973 - -0.5 281,973 - +0.9 281,973 - -0.1 281,973 - +0.2 281,973 - -0.1 281,973 - +0.0 281,973 - -0.1 281,973 - +1.5 281,973 - -1.1 281,974 + +0.6 281,975 - +1.7 282,192 - Psyr_0265 0.17 -0.2 282,369 + Psyr_0265 0.38 -0.5 282,370 - Psyr_0265 0.38 +0.9 282,605 + Psyr_0265 0.66 +0.5 282,605 + Psyr_0265 0.66 -0.1 282,605 + Psyr_0265 0.66 -0.1 282,605 + Psyr_0265 0.66 -0.8 282,605 + Psyr_0265 0.66 -1.0 282,605 + Psyr_0265 0.66 +1.4 282,605 + Psyr_0265 0.66 -0.2 282,605 + Psyr_0265 0.66 +1.5 282,606 - Psyr_0265 0.66 +0.3 282,606 - Psyr_0265 0.66 +1.5 282,606 - Psyr_0265 0.66 +0.2 282,606 - Psyr_0265 0.66 -0.6 282,606 - Psyr_0265 0.66 -1.0 282,606 - Psyr_0265 0.66 -0.4 282,638 + Psyr_0265 0.69 +0.8 282,638 + Psyr_0265 0.69 +0.5 282,639 - Psyr_0265 0.70 +0.5 282,667 + Psyr_0265 0.73 +0.5 282,668 - Psyr_0265 0.73 -0.2 282,668 - Psyr_0265 0.73 +0.8 282,701 + Psyr_0265 0.77 -1.6 282,701 + Psyr_0265 0.77 +1.1 282,701 + Psyr_0265 0.77 +0.1 282,701 + Psyr_0265 0.77 +0.2 282,701 + Psyr_0265 0.77 -0.2 282,701 + Psyr_0265 0.77 +0.3 282,701 + Psyr_0265 0.77 -0.3 282,701 + Psyr_0265 0.77 -0.2 282,701 + Psyr_0265 0.77 -0.4 282,702 - Psyr_0265 0.77 -0.3 282,702 - Psyr_0265 0.77 +0.1 282,702 - Psyr_0265 0.77 -0.1 282,702 - Psyr_0265 0.77 -0.2 282,702 - Psyr_0265 0.77 +0.9 282,702 - Psyr_0265 0.77 -0.9 282,702 - Psyr_0265 0.77 -0.5 282,702 - Psyr_0265 0.77 +0.5 282,702 - Psyr_0265 0.77 +0.9 282,702 - Psyr_0265 0.77 -0.5 282,702 - Psyr_0265 0.77 +0.4 282,716 + Psyr_0265 0.79 +0.1 282,716 + Psyr_0265 0.79 +0.7 282,716 + Psyr_0265 0.79 +0.8 282,716 + Psyr_0265 0.79 -1.1 282,716 + Psyr_0265 0.79 +0.1 282,717 - Psyr_0265 0.79 -0.9 282,717 - Psyr_0265 0.79 -1.0 282,717 - Psyr_0265 0.79 +0.5 282,811 + Psyr_0265 0.90 +0.0 282,811 + Psyr_0265 0.90 -0.1 282,815 + +0.1 282,815 + +0.8 282,815 + -0.1 282,815 + -1.6 282,816 - -0.8 282,816 - +0.0 282,816 - +0.9 282,816 - -2.4 282,870 + +0.3 282,872 + -1.4 282,873 - -1.4 282,878 + -0.4 282,878 + -1.1 282,878 + -0.5 282,878 + -1.6 282,878 + -0.8 282,878 + -0.1 282,878 + +0.2 282,878 + +0.2 282,878 + +0.9 282,878 + -0.1 282,878 + -0.6 282,878 + -0.5 282,879 - -1.1 282,879 - -1.8 282,879 - -0.9 282,879 - +0.0 282,879 - -0.8 282,879 - -0.5 282,879 - -0.5 282,879 - -0.4 282,879 - +2.1 282,879 - -1.4 282,879 - -0.3 282,964 + -0.1 282,964 + -0.0 282,965 - -0.5
Or see this region's nucleotide sequence