Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0168

Experiment: KB with Huperzine A 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0166 and Psyr_0167 are separated by 5 nucleotidesPsyr_0167 and Psyr_0168 are separated by 370 nucleotidesPsyr_0168 and Psyr_0169 are separated by 179 nucleotides Psyr_0166: Psyr_0166 - hypothetical protein, at 180,107 to 181,417 _0166 Psyr_0167: Psyr_0167 - hypothetical protein, at 181,423 to 181,845 _0167 Psyr_0168: Psyr_0168 - Phosphoenolpyruvate carboxykinase (ATP), at 182,216 to 183,760 _0168 Psyr_0169: Psyr_0169 - Hsp33 protein, at 183,940 to 184,842 _0169 Position (kb) 182 183 184Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 181.274 kb on + strand, within Psyr_0166at 181.286 kb on + strand, within Psyr_0166at 181.287 kb on - strandat 181.288 kb on + strandat 181.289 kb on - strandat 181.328 kb on - strandat 181.387 kb on + strandat 181.611 kb on + strand, within Psyr_0167at 181.693 kb on + strand, within Psyr_0167at 181.693 kb on + strand, within Psyr_0167at 181.693 kb on + strand, within Psyr_0167at 181.693 kb on + strand, within Psyr_0167at 181.694 kb on - strand, within Psyr_0167at 181.694 kb on - strand, within Psyr_0167at 181.696 kb on - strand, within Psyr_0167at 181.798 kb on - strand, within Psyr_0167at 181.843 kb on + strandat 181.847 kb on + strandat 181.847 kb on + strandat 181.847 kb on + strandat 181.848 kb on - strandat 181.848 kb on - strandat 181.904 kb on + strandat 181.904 kb on + strandat 181.904 kb on + strandat 181.905 kb on - strandat 182.034 kb on - strandat 182.054 kb on - strandat 182.084 kb on + strandat 182.086 kb on + strandat 182.087 kb on - strandat 182.087 kb on - strandat 182.104 kb on - strandat 182.128 kb on - strandat 182.207 kb on + strandat 182.207 kb on + strandat 182.208 kb on - strandat 182.217 kb on + strandat 182.218 kb on - strandat 182.218 kb on - strandat 182.313 kb on + strandat 182.314 kb on - strandat 182.532 kb on + strand, within Psyr_0168at 182.532 kb on + strand, within Psyr_0168at 182.532 kb on + strand, within Psyr_0168at 182.532 kb on + strand, within Psyr_0168at 182.532 kb on + strand, within Psyr_0168at 182.533 kb on - strand, within Psyr_0168at 182.533 kb on - strand, within Psyr_0168at 182.744 kb on + strand, within Psyr_0168at 182.835 kb on + strand, within Psyr_0168at 182.918 kb on + strand, within Psyr_0168at 182.989 kb on - strand, within Psyr_0168at 183.116 kb on + strand, within Psyr_0168at 183.116 kb on + strand, within Psyr_0168at 183.117 kb on - strand, within Psyr_0168at 183.117 kb on - strand, within Psyr_0168at 183.117 kb on - strand, within Psyr_0168at 183.117 kb on - strand, within Psyr_0168at 183.272 kb on + strand, within Psyr_0168at 183.272 kb on + strand, within Psyr_0168at 183.273 kb on - strand, within Psyr_0168at 183.366 kb on + strand, within Psyr_0168at 183.366 kb on + strand, within Psyr_0168at 183.366 kb on + strand, within Psyr_0168at 183.366 kb on + strand, within Psyr_0168at 183.366 kb on + strand, within Psyr_0168at 183.366 kb on + strand, within Psyr_0168at 183.366 kb on + strand, within Psyr_0168at 183.367 kb on - strand, within Psyr_0168at 183.367 kb on - strand, within Psyr_0168at 183.367 kb on - strand, within Psyr_0168at 183.367 kb on - strand, within Psyr_0168at 183.464 kb on + strand, within Psyr_0168at 183.464 kb on + strand, within Psyr_0168at 183.464 kb on + strand, within Psyr_0168at 183.464 kb on + strand, within Psyr_0168at 183.465 kb on - strand, within Psyr_0168at 183.465 kb on - strand, within Psyr_0168at 183.465 kb on - strand, within Psyr_0168at 183.465 kb on - strand, within Psyr_0168at 183.465 kb on - strand, within Psyr_0168at 183.634 kb on + strandat 183.635 kb on - strandat 183.677 kb on + strandat 183.678 kb on - strandat 183.735 kb on + strandat 183.735 kb on + strandat 183.735 kb on + strandat 183.735 kb on + strandat 183.736 kb on - strandat 183.736 kb on - strandat 183.736 kb on - strandat 183.736 kb on - strandat 183.827 kb on - strandat 183.833 kb on + strandat 183.834 kb on - strandat 183.898 kb on + strandat 183.898 kb on + strandat 183.898 kb on + strandat 183.899 kb on - strandat 183.899 kb on - strandat 184.017 kb on - strandat 184.403 kb on + strand, within Psyr_0169at 184.403 kb on + strand, within Psyr_0169at 184.403 kb on + strand, within Psyr_0169at 184.403 kb on + strand, within Psyr_0169at 184.404 kb on - strand, within Psyr_0169at 184.404 kb on - strand, within Psyr_0169at 184.404 kb on - strand, within Psyr_0169at 184.404 kb on - strand, within Psyr_0169at 184.751 kb on + strand, within Psyr_0169at 184.752 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM
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181,274 + Psyr_0166 0.89 -0.0
181,286 + Psyr_0166 0.90 +0.4
181,287 - +0.6
181,288 + +0.6
181,289 - -2.3
181,328 - -0.4
181,387 + +0.0
181,611 + Psyr_0167 0.44 +0.6
181,693 + Psyr_0167 0.64 -0.4
181,693 + Psyr_0167 0.64 +0.5
181,693 + Psyr_0167 0.64 +1.2
181,693 + Psyr_0167 0.64 +2.4
181,694 - Psyr_0167 0.64 -0.1
181,694 - Psyr_0167 0.64 -1.4
181,696 - Psyr_0167 0.65 +0.2
181,798 - Psyr_0167 0.89 -0.5
181,843 + -0.4
181,847 + -0.8
181,847 + -0.7
181,847 + -1.2
181,848 - -1.4
181,848 - -0.1
181,904 + -0.3
181,904 + -1.6
181,904 + +1.0
181,905 - -0.0
182,034 - -1.3
182,054 - -0.4
182,084 + +0.2
182,086 + +0.8
182,087 - +0.3
182,087 - -0.4
182,104 - -2.2
182,128 - +0.6
182,207 + -1.5
182,207 + -0.0
182,208 - +0.6
182,217 + +1.0
182,218 - -1.5
182,218 - +0.2
182,313 + +1.1
182,314 - -0.8
182,532 + Psyr_0168 0.20 +0.2
182,532 + Psyr_0168 0.20 -0.0
182,532 + Psyr_0168 0.20 +1.6
182,532 + Psyr_0168 0.20 +1.0
182,532 + Psyr_0168 0.20 +0.4
182,533 - Psyr_0168 0.21 +2.8
182,533 - Psyr_0168 0.21 +0.8
182,744 + Psyr_0168 0.34 +0.9
182,835 + Psyr_0168 0.40 +0.2
182,918 + Psyr_0168 0.45 -0.6
182,989 - Psyr_0168 0.50 +0.2
183,116 + Psyr_0168 0.58 -0.4
183,116 + Psyr_0168 0.58 +1.3
183,117 - Psyr_0168 0.58 +0.6
183,117 - Psyr_0168 0.58 -1.0
183,117 - Psyr_0168 0.58 +0.7
183,117 - Psyr_0168 0.58 +1.6
183,272 + Psyr_0168 0.68 -1.1
183,272 + Psyr_0168 0.68 -0.4
183,273 - Psyr_0168 0.68 -0.6
183,366 + Psyr_0168 0.74 -0.6
183,366 + Psyr_0168 0.74 -0.0
183,366 + Psyr_0168 0.74 +0.8
183,366 + Psyr_0168 0.74 -0.4
183,366 + Psyr_0168 0.74 -0.3
183,366 + Psyr_0168 0.74 +0.6
183,366 + Psyr_0168 0.74 -0.8
183,367 - Psyr_0168 0.74 -1.0
183,367 - Psyr_0168 0.74 -1.2
183,367 - Psyr_0168 0.74 -0.9
183,367 - Psyr_0168 0.74 -0.4
183,464 + Psyr_0168 0.81 +0.2
183,464 + Psyr_0168 0.81 +1.2
183,464 + Psyr_0168 0.81 -0.7
183,464 + Psyr_0168 0.81 +0.1
183,465 - Psyr_0168 0.81 +0.7
183,465 - Psyr_0168 0.81 -0.0
183,465 - Psyr_0168 0.81 -0.7
183,465 - Psyr_0168 0.81 -1.9
183,465 - Psyr_0168 0.81 +0.4
183,634 + +0.5
183,635 - -0.8
183,677 + +1.5
183,678 - +0.5
183,735 + +1.1
183,735 + -0.0
183,735 + +0.6
183,735 + +0.7
183,736 - -0.0
183,736 - +0.8
183,736 - -0.0
183,736 - +0.8
183,827 - -0.5
183,833 + +0.8
183,834 - +0.8
183,898 + -0.3
183,898 + -0.6
183,898 + +0.6
183,899 - +0.4
183,899 - +0.3
184,017 - -1.0
184,403 + Psyr_0169 0.51 -2.9
184,403 + Psyr_0169 0.51 +0.9
184,403 + Psyr_0169 0.51 -0.5
184,403 + Psyr_0169 0.51 +1.3
184,404 - Psyr_0169 0.51 +0.7
184,404 - Psyr_0169 0.51 +0.4
184,404 - Psyr_0169 0.51 -0.1
184,404 - Psyr_0169 0.51 -0.2
184,751 + Psyr_0169 0.90 -0.7
184,752 - +0.0

Or see this region's nucleotide sequence