Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0055 and Psyr_0056 are separated by 77 nucleotides Psyr_0056 and Psyr_0057 overlap by 10 nucleotides Psyr_0057 and Psyr_0058 are separated by 4 nucleotides Psyr_0058 and Psyr_0059 are separated by 216 nucleotides
Psyr_0055: Psyr_0055 - FKBP-type peptidyl-prolyl isomerase, N-terminal:Peptidylprolyl isomerase, FKBP-type, at 56,138 to 56,815
_0055
Psyr_0056: Psyr_0056 - Regulator of RNA polymerase sigma(70) subunit, Rsd/AlgQ, at 56,893 to 57,393
_0056
Psyr_0057: Psyr_0057 - conserved hypothetical protein, at 57,384 to 57,536
_0057
Psyr_0058: Psyr_0058 - Disulfide bond formation protein DsbB, at 57,541 to 58,068
_0058
Psyr_0059: Psyr_0059 - HemY, N-terminal:HemY, N-terminal:HemY, N-terminal, at 58,285 to 59,529
_0059
Position (kb) 57
58
59 Strain fitness (log2 ratio) -2
-1
0
1
2 at 56.579 kb on + strand, within Psyr_0055 at 56.580 kb on - strand, within Psyr_0055 at 56.580 kb on - strand, within Psyr_0055 at 56.580 kb on - strand, within Psyr_0055 at 56.732 kb on + strand, within Psyr_0055 at 56.732 kb on + strand, within Psyr_0055 at 56.732 kb on + strand, within Psyr_0055 at 56.733 kb on - strand, within Psyr_0055 at 56.771 kb on - strand at 56.782 kb on - strand at 56.782 kb on - strand at 56.813 kb on + strand at 56.813 kb on + strand at 56.813 kb on + strand at 56.814 kb on - strand at 56.814 kb on - strand at 56.814 kb on - strand at 56.856 kb on + strand at 56.856 kb on + strand at 56.856 kb on + strand at 56.856 kb on + strand at 56.857 kb on - strand at 56.857 kb on - strand at 56.857 kb on - strand at 56.857 kb on - strand at 56.873 kb on + strand at 56.873 kb on + strand at 56.873 kb on + strand at 56.873 kb on + strand at 56.874 kb on - strand at 56.874 kb on - strand at 56.874 kb on - strand at 56.928 kb on + strand at 56.928 kb on + strand at 56.929 kb on - strand at 57.092 kb on + strand, within Psyr_0056 at 57.161 kb on + strand, within Psyr_0056 at 57.161 kb on + strand, within Psyr_0056 at 57.161 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.269 kb on + strand, within Psyr_0056 at 57.269 kb on + strand, within Psyr_0056 at 57.270 kb on - strand, within Psyr_0056 at 57.270 kb on - strand, within Psyr_0056 at 57.416 kb on - strand, within Psyr_0057 at 57.416 kb on - strand, within Psyr_0057 at 57.416 kb on - strand, within Psyr_0057 at 57.416 kb on - strand, within Psyr_0057 at 57.416 kb on - strand, within Psyr_0057 at 57.438 kb on + strand, within Psyr_0057 at 57.439 kb on - strand, within Psyr_0057 at 57.439 kb on - strand, within Psyr_0057 at 57.439 kb on - strand, within Psyr_0057 at 57.439 kb on - strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.458 kb on - strand, within Psyr_0057 at 57.458 kb on - strand, within Psyr_0057 at 57.458 kb on - strand, within Psyr_0057 at 57.458 kb on - strand, within Psyr_0057 at 57.485 kb on + strand, within Psyr_0057 at 57.485 kb on + strand, within Psyr_0057 at 57.512 kb on + strand, within Psyr_0057 at 57.513 kb on - strand, within Psyr_0057 at 57.513 kb on - strand, within Psyr_0057 at 57.523 kb on + strand at 57.523 kb on + strand at 57.523 kb on + strand at 57.532 kb on + strand at 57.532 kb on + strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.624 kb on + strand, within Psyr_0058 at 57.624 kb on + strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.658 kb on - strand, within Psyr_0058 at 57.723 kb on + strand, within Psyr_0058 at 57.723 kb on + strand, within Psyr_0058 at 57.723 kb on + strand, within Psyr_0058 at 57.724 kb on - strand, within Psyr_0058 at 57.724 kb on - strand, within Psyr_0058 at 57.724 kb on - strand, within Psyr_0058 at 57.746 kb on - strand, within Psyr_0058 at 57.746 kb on - strand, within Psyr_0058 at 57.976 kb on + strand, within Psyr_0058 at 57.977 kb on - strand, within Psyr_0058 at 57.992 kb on + strand, within Psyr_0058 at 57.992 kb on + strand, within Psyr_0058 at 57.993 kb on - strand, within Psyr_0058 at 57.993 kb on - strand, within Psyr_0058 at 57.993 kb on - strand, within Psyr_0058 at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.099 kb on - strand at 58.194 kb on + strand at 58.194 kb on + strand at 58.194 kb on + strand at 58.194 kb on + strand at 58.195 kb on - strand at 58.195 kb on - strand at 58.195 kb on - strand at 58.206 kb on + strand at 58.206 kb on + strand at 58.206 kb on + strand at 58.206 kb on + strand at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.601 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.700 kb on + strand, within Psyr_0059 at 58.938 kb on - strand, within Psyr_0059 at 58.938 kb on - strand, within Psyr_0059
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 56,579 + Psyr_0055 0.65 +0.8 56,580 - Psyr_0055 0.65 +0.1 56,580 - Psyr_0055 0.65 -2.7 56,580 - Psyr_0055 0.65 -0.7 56,732 + Psyr_0055 0.88 -0.7 56,732 + Psyr_0055 0.88 +0.1 56,732 + Psyr_0055 0.88 -1.6 56,733 - Psyr_0055 0.88 +0.9 56,771 - -2.0 56,782 - +0.6 56,782 - +1.1 56,813 + -0.6 56,813 + -0.1 56,813 + -0.4 56,814 - -0.3 56,814 - -1.0 56,814 - -0.0 56,856 + +0.4 56,856 + +0.8 56,856 + +0.4 56,856 + -0.5 56,857 - +0.4 56,857 - +0.1 56,857 - -0.6 56,857 - -0.6 56,873 + -0.7 56,873 + -0.3 56,873 + +0.4 56,873 + -1.3 56,874 - -0.2 56,874 - +0.7 56,874 - +1.2 56,928 + -0.9 56,928 + +0.5 56,929 - -0.5 57,092 + Psyr_0056 0.40 +1.2 57,161 + Psyr_0056 0.53 -0.2 57,161 + Psyr_0056 0.53 -0.2 57,161 + Psyr_0056 0.53 +0.3 57,188 + Psyr_0056 0.59 -0.2 57,188 + Psyr_0056 0.59 -0.7 57,188 + Psyr_0056 0.59 +0.2 57,188 + Psyr_0056 0.59 +0.4 57,188 + Psyr_0056 0.59 -0.3 57,188 + Psyr_0056 0.59 -0.2 57,188 + Psyr_0056 0.59 -0.2 57,189 - Psyr_0056 0.59 -0.0 57,189 - Psyr_0056 0.59 -0.5 57,189 - Psyr_0056 0.59 -0.2 57,189 - Psyr_0056 0.59 -0.4 57,189 - Psyr_0056 0.59 +0.1 57,269 + Psyr_0056 0.75 -1.8 57,269 + Psyr_0056 0.75 -2.4 57,270 - Psyr_0056 0.75 +0.4 57,270 - Psyr_0056 0.75 +0.9 57,416 - Psyr_0057 0.21 +2.2 57,416 - Psyr_0057 0.21 +2.1 57,416 - Psyr_0057 0.21 -1.7 57,416 - Psyr_0057 0.21 +0.3 57,416 - Psyr_0057 0.21 -0.7 57,438 + Psyr_0057 0.35 -1.1 57,439 - Psyr_0057 0.36 +0.6 57,439 - Psyr_0057 0.36 -0.1 57,439 - Psyr_0057 0.36 -0.4 57,439 - Psyr_0057 0.36 -0.1 57,457 + Psyr_0057 0.48 +1.2 57,457 + Psyr_0057 0.48 +0.1 57,457 + Psyr_0057 0.48 -0.2 57,457 + Psyr_0057 0.48 +0.7 57,457 + Psyr_0057 0.48 -0.0 57,457 + Psyr_0057 0.48 -0.7 57,457 + Psyr_0057 0.48 +0.2 57,457 + Psyr_0057 0.48 +0.4 57,457 + Psyr_0057 0.48 -0.4 57,458 - Psyr_0057 0.48 +1.6 57,458 - Psyr_0057 0.48 +0.8 57,458 - Psyr_0057 0.48 -0.0 57,458 - Psyr_0057 0.48 -0.7 57,485 + Psyr_0057 0.66 -0.3 57,485 + Psyr_0057 0.66 -0.2 57,512 + Psyr_0057 0.84 -1.9 57,513 - Psyr_0057 0.84 -0.2 57,513 - Psyr_0057 0.84 -0.2 57,523 + -0.5 57,523 + +0.8 57,523 + -0.1 57,532 + -0.7 57,532 + +1.3 57,533 - +1.2 57,533 - -0.4 57,533 - +0.2 57,533 - -0.3 57,533 - +0.5 57,533 - +0.4 57,624 + Psyr_0058 0.16 -0.2 57,624 + Psyr_0058 0.16 +0.3 57,625 - Psyr_0058 0.16 -1.1 57,625 - Psyr_0058 0.16 -0.8 57,625 - Psyr_0058 0.16 +0.8 57,625 - Psyr_0058 0.16 -0.5 57,625 - Psyr_0058 0.16 +0.6 57,625 - Psyr_0058 0.16 +0.7 57,625 - Psyr_0058 0.16 -2.0 57,625 - Psyr_0058 0.16 +1.1 57,658 - Psyr_0058 0.22 -0.7 57,723 + Psyr_0058 0.34 +0.8 57,723 + Psyr_0058 0.34 -0.7 57,723 + Psyr_0058 0.34 -1.2 57,724 - Psyr_0058 0.35 -2.0 57,724 - Psyr_0058 0.35 -1.8 57,724 - Psyr_0058 0.35 +0.7 57,746 - Psyr_0058 0.39 -1.4 57,746 - Psyr_0058 0.39 -0.5 57,976 + Psyr_0058 0.82 -0.6 57,977 - Psyr_0058 0.83 -1.1 57,992 + Psyr_0058 0.85 -0.2 57,992 + Psyr_0058 0.85 +0.1 57,993 - Psyr_0058 0.86 -1.3 57,993 - Psyr_0058 0.86 +0.0 57,993 - Psyr_0058 0.86 -0.6 58,064 + +0.4 58,064 + -0.1 58,064 + +2.2 58,064 + -0.3 58,064 + +0.1 58,064 + +0.6 58,064 + -0.9 58,064 + -1.3 58,064 + -0.1 58,064 + +2.2 58,064 + +0.8 58,065 - -0.6 58,065 - -0.1 58,065 - -0.5 58,065 - +1.0 58,065 - -0.9 58,065 - +0.3 58,065 - +0.8 58,065 - +1.4 58,065 - -1.3 58,065 - -0.2 58,065 - -0.2 58,065 - -0.3 58,065 - -0.4 58,065 - -1.1 58,065 - +0.9 58,065 - -0.1 58,065 - +0.6 58,065 - -1.6 58,099 - +0.8 58,194 + +0.5 58,194 + +0.3 58,194 + -0.2 58,194 + +0.7 58,195 - +1.6 58,195 - -0.9 58,195 - +0.2 58,206 + +1.2 58,206 + -1.0 58,206 + +0.7 58,206 + +1.2 58,497 + Psyr_0059 0.17 +0.3 58,497 + Psyr_0059 0.17 -0.3 58,497 + Psyr_0059 0.17 -1.4 58,497 + Psyr_0059 0.17 -1.2 58,497 + Psyr_0059 0.17 -0.0 58,497 + Psyr_0059 0.17 +0.1 58,497 + Psyr_0059 0.17 -0.8 58,497 + Psyr_0059 0.17 -0.4 58,497 + Psyr_0059 0.17 +0.7 58,498 - Psyr_0059 0.17 +0.5 58,498 - Psyr_0059 0.17 +1.4 58,498 - Psyr_0059 0.17 +2.2 58,498 - Psyr_0059 0.17 +1.3 58,498 - Psyr_0059 0.17 -0.2 58,498 - Psyr_0059 0.17 -1.0 58,498 - Psyr_0059 0.17 +0.1 58,498 - Psyr_0059 0.17 -0.1 58,498 - Psyr_0059 0.17 -0.1 58,498 - Psyr_0059 0.17 -0.4 58,498 - Psyr_0059 0.17 -0.1 58,498 - Psyr_0059 0.17 -0.7 58,498 - Psyr_0059 0.17 -2.4 58,601 + Psyr_0059 0.25 -1.4 58,625 + Psyr_0059 0.27 +1.2 58,625 + Psyr_0059 0.27 -0.5 58,625 + Psyr_0059 0.27 -0.4 58,625 + Psyr_0059 0.27 -0.1 58,625 + Psyr_0059 0.27 -1.0 58,700 + Psyr_0059 0.33 -1.2 58,938 - Psyr_0059 0.52 -0.2 58,938 - Psyr_0059 0.52 +0.9
Or see this region's nucleotide sequence