Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22320 and BWI76_RS22325 are separated by 17 nucleotides BWI76_RS22325 and BWI76_RS22330 are separated by 2 nucleotides BWI76_RS22330 and BWI76_RS22335 overlap by 4 nucleotides BWI76_RS22335 and BWI76_RS22340 are separated by 82 nucleotides
BWI76_RS22320: BWI76_RS22320 - hydrogenase 3 large subunit, at 4,652,151 to 4,653,860
_RS22320
BWI76_RS22325: BWI76_RS22325 - hydrogenase 3 membrane subunit, at 4,653,878 to 4,654,801
_RS22325
BWI76_RS22330: BWI76_RS22330 - formate hydrogenlyase subunit 3, at 4,654,804 to 4,656,630
_RS22330
BWI76_RS22335: BWI76_RS22335 - 4Fe-4S ferredoxin, at 4,656,627 to 4,657,235
_RS22335
BWI76_RS22340: BWI76_RS22340 - formate hydrogenlyase regulatory protein HycA, at 4,657,318 to 4,657,767
_RS22340
Position (kb) 4654
4655
4656
4657 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4653.956 kb on - strand at 4654.110 kb on + strand, within BWI76_RS22325 at 4654.128 kb on - strand, within BWI76_RS22325 at 4654.312 kb on - strand, within BWI76_RS22325 at 4654.328 kb on + strand, within BWI76_RS22325 at 4654.367 kb on - strand, within BWI76_RS22325 at 4654.576 kb on + strand, within BWI76_RS22325 at 4654.862 kb on + strand at 4655.163 kb on - strand, within BWI76_RS22330 at 4655.294 kb on - strand, within BWI76_RS22330 at 4655.321 kb on - strand, within BWI76_RS22330 at 4655.682 kb on - strand, within BWI76_RS22330 at 4655.909 kb on + strand, within BWI76_RS22330 at 4656.349 kb on - strand, within BWI76_RS22330 at 4656.400 kb on + strand, within BWI76_RS22330 at 4656.438 kb on + strand, within BWI76_RS22330 at 4656.474 kb on - strand at 4656.770 kb on - strand, within BWI76_RS22335 at 4656.836 kb on + strand, within BWI76_RS22335 at 4656.849 kb on - strand, within BWI76_RS22335 at 4656.883 kb on + strand, within BWI76_RS22335 at 4656.921 kb on - strand, within BWI76_RS22335 at 4657.292 kb on - strand at 4657.609 kb on - strand, within BWI76_RS22340
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 4,653,956 - +0.1 4,654,110 + BWI76_RS22325 0.25 +0.0 4,654,128 - BWI76_RS22325 0.27 -0.5 4,654,312 - BWI76_RS22325 0.47 -0.3 4,654,328 + BWI76_RS22325 0.49 +1.8 4,654,367 - BWI76_RS22325 0.53 -0.8 4,654,576 + BWI76_RS22325 0.76 +0.5 4,654,862 + +0.6 4,655,163 - BWI76_RS22330 0.20 +0.6 4,655,294 - BWI76_RS22330 0.27 -0.2 4,655,321 - BWI76_RS22330 0.28 -0.5 4,655,682 - BWI76_RS22330 0.48 -0.0 4,655,909 + BWI76_RS22330 0.60 +0.0 4,656,349 - BWI76_RS22330 0.85 +0.2 4,656,400 + BWI76_RS22330 0.87 +0.6 4,656,438 + BWI76_RS22330 0.89 +0.0 4,656,474 - -0.5 4,656,770 - BWI76_RS22335 0.23 +0.7 4,656,836 + BWI76_RS22335 0.34 +0.2 4,656,849 - BWI76_RS22335 0.36 +0.2 4,656,883 + BWI76_RS22335 0.42 -2.0 4,656,921 - BWI76_RS22335 0.48 +0.3 4,657,292 - -1.0 4,657,609 - BWI76_RS22340 0.65 +0.0
Or see this region's nucleotide sequence